GEO DataSets

(Submitter supplied) We sought understand the molecular mehcanism of gene regulation by Mga and its contribution to GAS pathogenesis in serotype M59 GAS through whole transcriptome analysis of strains with phosphorylation mimicking substitutions in key histidine residues of Mga.

Organism:

Streptococcus pyogenes MGAS15252

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20059

18 Samples

Download data: XLSX

(Submitter supplied) Purpose: We used dual RNA-seq to analyze the transcriptomes of serotype M1 S. pyogenes and host skeletal muscle recovered contemporaneously from infected nonhuman primates (NHPs). We identified genes important for necrotizing myositis, made isogenic deletion mutants and performed RNA-seq comparing one of those deletion mutant strains (dahA) grown in vitro and compared to its parental wild-type strain. more...

Organism:

Streptococcus pyogenes; Macaca fascicularis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

54 Samples

Download data: XLSX

(Submitter supplied) We investigated the covS-regulation of S. pyogenes in a hypervirulent M23 strain using RNA-sequencing. The differential gene expression comparison between the covS- mutant and isogenic wild type covS+ identified altered expression of 349 (18%) genes, including a broad spectrum of virulence genes and diverse metabolic genes. The data showed that the strain achieved hypervirulence by enhancing the expression of genes responsible for invasiveness and antiphagocytosis (i.e., hasABC), by abrogating the expression of toxic genes (i.e., speB), and by compromising gene products with dispensable functions (i.e., sfb1). more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

8 Samples

Download data: XLS

(Submitter supplied) We sought to determine how CovRS mutations varying CovR phosphorylation levels affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

32 Samples

Download data: TXT

(Submitter supplied) We sought to determine how single amino acid changes in the active site of CovS autophosphorylation and subsequent phosphorylation of the response regulator CovR affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

24 Samples

Download data: TXT

(Submitter supplied) GAS is a highly virulent Gram-positive bacterium. For the successful infection GAS express many virulence factors, which are clustered together with transcriptional regulators in distinct genomic regions. Ralp3 is a central regulator of the ERES region. In this study, we investigated the role of Ralp3 in GAS pathogenesis. To characterize the Ralp3 regulatory function on the whole genome level, GAS M49 wild type and Äralp3 mutant strains were comprehensively compared by two colour microarray analysis. more...

Organism:

Streptococcus pyogenes M49 591; Streptococcus pyogenes NZ131

Type:

Expression profiling by array

Platform:

GPL13816

16 Samples

Download data: TXT

(Submitter supplied) RNA-seq experiments of a wild-type and isogenic rocA deletion mutant serotype M28 group A streptococcus strain at two time points

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24880

12 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing

Platforms:

GPL26590 GPL24912

7 Samples

Download data: HTML, TXT

(Submitter supplied) We used Pacific Biosciences Single Molecule Real-Time sequencing platform to identify modified motifs in an RM deficient strain of Streptococcus pyogenes

Organism:

Streptococcus pyogenes

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL26590

1 Sample

Download data: HTML, TXT

(Submitter supplied) We used Illumina NGS to measure mRNA levels and perform subsequent differential expression analysis between wild type and RM system deficient strains of Streptococcus pyogenes

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24912

6 Samples

Download data: CSV, TXT

(Submitter supplied) RNA-seq experiments of serotype M28 group A streptococcus strains with mutations in rocA at two time points

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24880

257 Samples

Download data: XLSX

(Submitter supplied) The aim of this study was to determine the extent of the trxR regulon in the M1 serotype MGAS5005. Gold, Leday, McIver 2008 Keywords: Wild-type vs trxR-

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL1482

6 Samples

Download data: GPR

(Submitter supplied) The goal was to perform global transcriptome analysis on isogenic wild type 2221 and FabT deletion mutant M1 strains to investigate how inactivation of the fabT gene affects gene expression.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

24 Samples

Download data: XLSX

(Submitter supplied) The control of virulence two-component gene regulatory system (CovRS) is critical to the pathogenesis of many medically important streptococci. In emm1 group A streptococci (GAS), CovR directly binds the promoters of numerous GAS virulence factor encoding genes. Elimination of CovS phosphatase activity increases CovR phosphorylation (CovR~P) levels and abrogates GAS virulence. Given the emm type-specific diversity of CovRS function, herein we used ChIP-seq to define global CovR DNA occupancy in the wild-type emm3 strain MGAS10870 (medium CovR~P) and its CovS phosphatase-negative derivative 10870-CovS-T284A (high CovR~P). more...

Organism:

Streptococcus pyogenes

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL28678

11 Samples

Download data: XLSX

(Submitter supplied) We report the mRNA expression profile of Streptococcus pyogeens Type M1 strain M1T15448-derived mutant lacking phospotyrosine phosphatase.

Organism:

Streptococcus pyogenes

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT

(Submitter supplied) The mechanisms by which bacteria sense the host environment and alter gene expression are poorly understood. LiaFSR is a gene regulatory system unique to Gram-positive bacteria including group A Streptococcus (GAS) and responds to cell envelope stress. We previously showed that LiaF acts as an inhibitor to LiaFSR activation in GAS. To better understand gene regulation associated with LiaFSR activation, we performed RNA-sequencing on isogenic deletion mutants fixed in a LiaFSR “always on” (DliaF) or “always off” (DliaR) state. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24912

9 Samples

Download data: XLSX

(Submitter supplied) We sought to determine how deletion of ccpA affects the gene expression profile in three M serotype strains of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

24 Samples

Download data: TXT

(Submitter supplied) Recent whole-genome sequencing of large populations of the same bacterial species has revealed significant disparity among genes in the frequency of single nucleotide polymorphisms (SNPs). For example, a previous analysis of invasive serotype M3 group A streptococci (GAS) found the highest frequency of SNPs in the gene (ropB) encoding the regulator of proteinase B (RopB). This finding led us to hypothesize that RopB polymorphisms contribute to altered GAS host-pathogen interactions. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL9080

8 Samples

Download data: CEL

(Submitter supplied) We used RNA-Seq to assess whether the rocA gene regulates gene expression in M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20656

6 Samples

Download data: XLSX

(Submitter supplied) We used RNA-Seq to assess whether the rocA gene regulates gene expression in M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

3 Samples

Download data: XLS