GEO DataSets

(Submitter supplied) Aged humans and rodents are susceptible to infection with Streptococcus pneumoniae bacteria as a result of an inability to make antibodies to capsular polysaccharides. This is partly a result of decreased production of proinflammatory cytokines and increased production of interleukin (IL)-10 by macrophages (Mphi) from aged mice. To understand the molecular basis of cytokine dysregulation in aged mouse Mphi, a microarray analysis was performed on RNA from resting and lipopolysaccharide (LPS)-stimulated Mphi from aged and control mice using the Affymetrix Mouse Genome 430 2.0 gene chip. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) Gene expression affected by TNFa was investigated, then, p38 inhibitor SB203580 was used to investigate p38 signal pathway. Keywords: p38 signal response

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS2885

Platform:

GPL2877

14 Samples

Download data: TXT

Analysis of fibroblast-like synoviocytes (FLS) treated with TGFalpha alone or in combination with SB203580, an inhibitor of p38. FLS, targets of proinflammatory cytokines, are involved in rheumatoid arthritis (RA). Results provide insight into the role of the p38 pathway in RA pathogenesis.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 4 agent, 2 protocol sets

Platform:

GPL2877

Series:

GSE7826

14 Samples

Download data: TXT

(Submitter supplied) Lipopolysaccharide (LPS)/immunue complex (IC) stimulated macrophages produce cytokine profiles that differe from LPS-stimulated inflammatory macropahges. Notch signaling is activated in LPS/IC-stimulated macrophages and inhibition of Notch signaling reduced IL-10 production. This study investigated the effect of gamma-secretase inhibitor (GSI) that suppresses Notch signaling pathway, on gene expression profiles in macrophages activated by LPS/IC.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19057

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL13112 GPL17940

38 Samples

Download data: CEL

(Submitter supplied) When macrophages encounter pathogens, they transiently induce an orchestrated cascade of pro- and anti-inflammatory genes. We systematically analyzed the contribution of translational regulation to the early phase of macrophage activation. While the expression of most cytokines is regulated by changes in mRNA levels, de-repression of translation was found to permit expression of many feedback inhibitors of the inflammatory response. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17940

30 Samples

Download data: CEL

(Submitter supplied) When macrophages encounter pathogens, they transiently induce an orchestrated cascade of pro- and anti-inflammatory genes. To obtain a precise picture of transcriptome-wide mRNA expression patterns, we performed RNA-Seq of total RNA at a high temporal resolution during the first two hours of macrophage activation. We systematically analyzed the contribution of translational regulation to the early phase of macrophage activation. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

8 Samples

Download data: TXT

(Submitter supplied) Background: High density lipoprotein (HDL) protects the artery wall by removing cholesterol from lipid-laden macrophages. However, recent evidence suggests that it might also inhibit atherogenesis by combating inflammation. Methods and Results: To identify potential anti-inflammatory mechanisms, we challenged macrophages with lipopolysaccharide (LPS), an inflammatory microbial ligand for Toll-like receptor 4 (TLR4). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

12 Samples

Download data: CEL

(Submitter supplied) Our data suggest that serum miR-204-3p has a potential as a useful biomarker among patients with FMF and that miR-204-3p plays a critical role as a suppressor to regulate the production of TLR4-related cytokines by targeting PI3K signaling pathway.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20844

4 Samples

Download data: TXT

(Submitter supplied) We compared the gene expression patterns of macrophages infected with S. oralis wild type and SpxB KO, a strain that does not produce H2O2.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL21810

8 Samples

Download data: TXT

(Submitter supplied) Located in the perisinusoidal space of Disse, hepatic stellate cells (HSCs) communicate with all other liver cell types by physical association and / or by producing cytokines and chemokines. In liver disease and folllowing liver transplantation, elevated levels of endotoxin (bacterial lipopolysaccharide: LPS) stimulate HSCs to produce increased amounts of cytokines and chemokines. Transcriptomic analysis of cultured HSCs stimulated with LPS yields a survey of expression changes which potentially modulate the hepatic inflammatory and immune responses.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS5251 GDS5252

Platform:

GPL1355

11 Samples

Download data: CEL, TXT

Analysis of hepatic stellate cells (HSCs) cultured with LPS for 1, 3, 6, 12, 24 hr. During exposure to toxins such as LPS, the liver suffers inflammatory injury. Results provide insight into molecular mechanisms underlying hepatic inflammatory and immune responses.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 5 time sets

Platform:

GPL1355

Series:

GSE49980

11 Samples

Download data: CEL

Analysis of hepatic stellate cells (HSCs) cultured with LPS for 1 and 24 hr. During exposure to toxins such as LPS, the liver suffers inflammatory injury. Results provide insight into molecular mechanisms underlying hepatic inflammatory and immune responses.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 2 time sets

Platform:

GPL1355

Series:

GSE49980

8 Samples

Download data: CEL

(Submitter supplied) These microarrays were performed for use in a genome-wide scan for LPS-regulated genes in mouse macrophages, in order to construct a list of LPS-regulated genes for detailed interrogation on custom microarrays (see GSE19490 for custom array analysis).

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4055

11 Samples

Download data: TXT

(Submitter supplied) These microarrays were performed for use in a genome-wide scan for LPS-regulated genes in human monocyte-derived macrophages, in order to construct a list of LPS-regulated genes for detailed interrogation on custom microarrays (see GSE19482 for custom array analysis).

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

10 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Homo sapiens

Type:

Expression profiling by array

4 related Platforms

61 Samples

Download data: TXT

(Submitter supplied) Evolutionary change in gene expression is generally considered to be a major driver of phenotypic differences between species. We investigated innate immune diversification by analyzing inter-species differences in the transcriptional responses of primary human and mouse macrophages to the TLR4 agonist, LPS. Using a custom platform permitting cross-species interrogation coupled with deep sequencing of mRNA 5’ ends, we identified extensive divergence in LPS-regulated orthologous gene expression between humans and mice (24% of orthologs, http://www.macgate.qfab.org). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL9802

24 Samples

Download data: TXT

(Submitter supplied) Evolutionary change in gene expression is generally considered to be a major driver of phenotypic differences between species. We investigated innate immune diversification by analyzing inter-species differences in the transcriptional responses of primary human and mouse macrophages to the TLR4 agonist, LPS. Using a custom platform permitting cross-species interrogation coupled with deep sequencing of mRNA 5’ ends, we identified extensive divergence in LPS-regulated orthologous gene expression between humans and mice (24% of orthologs, http://www.macgate.qfab.org). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL9801

16 Samples

Download data: TXT

(Submitter supplied) 60-mer spotted oligonucleotide probes. Protocol: See manufacturer's website (www.agilent.com).

Organism:

Mus musculus

2 Series

24 Samples

Download data

(Submitter supplied) 60-mer spotted oligonucleotide probes. Protocol: See manufacturer's website (www.agilent.com).

Organism:

Homo sapiens

2 Series

16 Samples

Download data