GEO DataSets

(Submitter supplied) The interferon regulatory factors IRF3 and IRF7 are key players in the regulation of type I and III IFN genes. In this study, we analyzed the role of IRF3 and IRF7 for the host response to influenza A virus infections in Irf3-/-, Irf7-/- and Irf3-/-Irf7-/- knock-out mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11202

24 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

62 Samples

Download data: CEL

(Submitter supplied) We used microarrays to detail the global programme of gene expression in response to Influenza A (PR8) infection

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL, TXT

(Submitter supplied) We used microarrays to detail the global programme of gene expression in response to Influenza A (PR8) infection

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

40 Samples

Download data: CEL, TXT

(Submitter supplied) We used microarrays to detail the global programme of gene expression in response to Influenza A (PR8) infection

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

10 Samples

Download data: CEL, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

35 Samples

Download data

(Submitter supplied) The RIG-I like receptor pathway is stimulated during RNA virus infection by interaction between cytosolic RIG-I and viral RNA structures that contain short hairpin dsRNA and 5’ triphosphate (5’ppp) terminal structure. In the present study, an RNA agonist of RIG-I was synthesized in vitro and shown to stimulate RIG-I-dependent antiviral responses at concentrations in the picomolar range. In human lung epithelial A549 cells, 5’pppRNA specifically stimulated multiple parameters of the innate antiviral response, including IRF3, IRF7 and STAT1 activation, andinduction of inflammatory and interferon stimulated genes - hallmarks of a fully functional antiviral response. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

11 Samples

Download data: TXT

(Submitter supplied) The RIG-I like receptor pathway is stimulated during RNA virus infection by interaction between cytosolic RIG-I and viral RNA structures that contain short hairpin dsRNA and 5’ triphosphate (5’ppp) terminal structure. In the present study, an RNA agonist of RIG-I was synthesized in vitro and shown to stimulate RIG-I-dependent antiviral responses at concentrations in the picomolar range. In human lung epithelial A549 cells, 5’pppRNA specifically stimulated multiple parameters of the innate antiviral response, including IRF3, IRF7 and STAT1 activation, andinduction of inflammatory and interferon stimulated genes - hallmarks of a fully functional antiviral response. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

24 Samples

Download data: TXT

(Submitter supplied) Airway epithelial cells and macrophages differ markedly in their responses to influenza A virus (IAV) infection. To investigate transcriptional responses underlying these differences, purified subsets of type II airway epithelial cells (ATII) and alveolar macrophages (AM) recovered from the lungs of mock- or IAV-infected mice were subjected to RNA sequencing. In the absence of infection, AM predominantly expressed genes related to immunity whereas ATII expressed genes consistent with their physiological roles in the lung. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

96 Samples

Download data: TXT

(Submitter supplied) To elucidate the functional role of chicken IRF7 against avian influenza virus (AIV) infection, we generated inducible IRF7 knockout DF-1 cell lines and performed in vitro infection using low pathogenic AIVs (LPAIVs) followed by RNA-seq.

Organism:

Gallus gallus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23499

24 Samples

Download data: CSV

(Submitter supplied) Background: Influenza A virus (IAV) infections periodically cause substantial morbidity and mortality in the human population. In the lung, the primary targets for IAV replication are type II alveolar epithelial cells (AECII), which are increasingly recognized for their immunological potential. However, our knowledge of the role of AECII in anti-IAV immunity is incomplete and their in vivo response to infection has not been evaluated. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

28 Samples

Download data: CEL

(Submitter supplied) The Interferon Regulatory Factors (IRFs) are essential regulators of the innate immune response to viruses. IRFs 3, 5, and 7 drive type­-1­-interferon and cytokine production in a cell-­type and stimulus­-specific manner, suggesting regulatory complexity. IRFs ­3, ­5 and ­7 bind DNA as phosphorylation-­activated dimers and are classically described as binding a doublet of the canonical IRF binding site: 5'-­AANNGAAA­-3’. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL24506

14 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL13112 GPL15408

15 Samples

Download data: BAR, BED, CEL, TXT

(Submitter supplied) We have used an unbiased systems approach to predict that a member of the forkhead family of transcription factors, FOXO3, is a negative regulator of a subset of antiviral genes. This prediction was validated using macrophages isolated from Foxo3-null mice. We detected significantly increased transcription of a subset of interferon-stimulated genes (ISGs) under basal conditions in Foxo3-null macrophages when compared to their wild type (WT) counterparts, suggesting that FOXO3 functions as a repressor of these genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL15408

12 Samples

Download data: CEL

(Submitter supplied) We predict that a member of the forkhead family of transcription factors, FOXO3, is a negative regulator of a subset of antiviral genes. This prediction was validated using macrophages isolated from Foxo3-null mice. Genome-wide location analysis combined with gene deletion studies identified the Irf7 gene as a critical target of FOXO3. FOXO3 was identified as a negative regulator of Irf7 transcription. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

3 Samples

Download data: BAR, BED, TXT

(Submitter supplied) Viral inflammation contributes to pathogenesis and mortality during respiratory virus infections. We recently uncovered Irf3, a critical component of innate antiviral immune responses, interacts with pro-inflammatory transcription factor NF-kB, and inhibits its activity. Irf3, using this mechanism, suppresses inflammatory gene expression in virus-infected cells and mice. In this study, we evaluated the cells responsible for Irf3-mediated suppression of viral inflammation using newly engineered conditional Irf3Δ/Δ mice. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24247

12 Samples

Download data: XLS

(Submitter supplied) Temporal changes of the expression levels of the complete human transcriptome during the first 24 hours following infection of IFN-pre-treated macrophages. This approach has allowed us to identify genes involved in the IFN signaling that have an impact on HIV-1 infection of macrophages KEYWORDS: time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4232

Platform:

GPL570

17 Samples

Download data: CEL

Temporal analysis of macrophages pretreated for 18hrs with interferon alpha 2 (IFNα2) then infected with HIV-1 (Bal strain). IFN treatment prior to in vitro HIV infection inhibits virus replication. Results provide insight into molecular mechanisms underlying this modulation of viral replication.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 infection, 2 protocol, 6 time sets

Platform:

GPL570

Series:

GSE30536

17 Samples

Download data: CEL

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. Methods: spleen mRNA profiles of 8-weeks-old WT and lncRNA-ISIR, used to be called lnRNA-IRF3, knockout mice were generated by deep sequencing, using BGISEQ-500 system. The sequence reads that passed quality filters were analyzed at the transcriptional level with RSEM (RNA-seq by Expectation Maximization). more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

2 Samples

Download data: TXT

(Submitter supplied) Flag-taged IRF3-binding RNAs were analyzed by imprinting RNA-sequencing of anti-flag antibody-retrieved complexes from macrophages lysate.

Organism:

Mus musculus

Type:

Other

Platform:

GPL21103

2 Samples

Download data: TDF