GEO DataSets

(Submitter supplied) Manuscript title: The endopeptidase PepO regulates the SpeB cysteine protease and is essential for the virulence of invasive M1T1 Streptococcus pyogenes. The study investigates the effect of pepO mutation on global gene expression in GAS M1T1 strain 5448.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL19152

3 Samples

Download data: TXT, XLSX

(Submitter supplied) Group A streptococci (GAS) may engage different sets of virulence strategies, depending on the site of infection and host context. We previously isolated 2 phenotypic variants of a globally disseminated M1T1 GAS clone: a virulent wild-type (WT) strain, characterized by a SpeB(+)/SpeA(-)/Sda1(low) phenotype, and a hypervirulent animal-passaged (AP) strain, better adapted to survive in vivo, with a SpeB(-)/SpeA(+)/Sda1(high) phenotype. more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10950

40 Samples

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(Submitter supplied) A total of 215 gene were found to be differentially expressed; 90 of them were up regulated and 125 genes were down regulated. Most notably the functional categories that were affected include Virulence genes, carbohydrate genes and lipid synthesis genes. Some of the virulence genes were down regulated by more than 16-32 fold. These results indicate that SDH plays an important role in the regulation of virulence.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL8290

8 Samples

Download data: GPR

(Submitter supplied) Microarray analysis was performed on in vivo-derived RNA from the well characterized GAS strain 5448 and its derivative 5448R-, containing the mutated ropB allele from 5628.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL7661

7 Samples

Download data: GPR

(Submitter supplied) We investigated the covS-regulation of S. pyogenes in a hypervirulent M23 strain using RNA-sequencing. The differential gene expression comparison between the covS- mutant and isogenic wild type covS+ identified altered expression of 349 (18%) genes, including a broad spectrum of virulence genes and diverse metabolic genes. The data showed that the strain achieved hypervirulence by enhancing the expression of genes responsible for invasiveness and antiphagocytosis (i.e., hasABC), by abrogating the expression of toxic genes (i.e., speB), and by compromising gene products with dispensable functions (i.e., sfb1). more...

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19982

8 Samples

Download data: XLS

(Submitter supplied) S. pyogenes strains were compared with the intact covRS form of the globally disseminated M1T1 clone to track transcriptomic changes engendered during the emergence of the M1T1 clone. The mutant covRS form of the M1T1 clone was included as a transcriptomic outlier and to provide a context for the magnitude of transcriptional shifts detected within the isolate set examined.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10822

36 Samples

Download data: GPR

(Submitter supplied) The speB gene of Streptococcus pyogenes has a long 5' UTR with unknown functions. Preliminary studies showed that the partial mutation of this region led to altered expression patterns of the speB gene and ropB, its upstream gene. In this study, we used microarray to examine the effect of speB 5' UTR mutation on the S. pyogenes transcriptome.

Organism:

Streptococcus pyogenes NZ131; Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL11420

12 Samples

Download data: CEL, CHP

(Submitter supplied) RNA-seq experiments of a wild-type and isogenic rocA deletion mutant serotype M28 group A streptococcus strain at two time points

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24880

12 Samples

Download data: XLSX

(Submitter supplied) Purpose: We used dual RNA-seq to analyze the transcriptomes of serotype M1 S. pyogenes and host skeletal muscle recovered contemporaneously from infected nonhuman primates (NHPs). We identified genes important for necrotizing myositis, made isogenic deletion mutants and performed RNA-seq comparing one of those deletion mutant strains (dahA) grown in vitro and compared to its parental wild-type strain. more...

Organism:

Streptococcus pyogenes; Macaca fascicularis

Type:

Expression profiling by high throughput sequencing

4 related Platforms

54 Samples

Download data: XLSX

(Submitter supplied) We sought to determine how CovRS mutations varying CovR phosphorylation levels affect the gene expression profile of group A streptococcus

Organism:

Streptococcus sp. 'group A'

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19691

32 Samples

Download data: TXT

(Submitter supplied) GAS is a highly virulent Gram-positive bacterium. For the successful infection GAS express many virulence factors, which are clustered together with transcriptional regulators in distinct genomic regions. Ralp3 is a central regulator of the ERES region. In this study, we investigated the role of Ralp3 in GAS pathogenesis. To characterize the Ralp3 regulatory function on the whole genome level, GAS M49 wild type and Äralp3 mutant strains were comprehensively compared by two colour microarray analysis. more...

Organism:

Streptococcus pyogenes M49 591; Streptococcus pyogenes NZ131

Type:

Expression profiling by array

Platform:

GPL13816

16 Samples

Download data: TXT

(Submitter supplied) The rivR gene encodes a putative transcriptional regulator, while the rivX gene encodes a putative small regulatory RNA. To determine the RivRX regulon during exponential phase growth in THY broth we compared parental strain MGAS2221 with isogenic rivRX mutant strain 2221ΔrivRX.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL9080

4 Samples

Download data: CEL

(Submitter supplied) RNA-seq experiments of serotype M28 group A streptococcus strains with mutations in rocA at two time points

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL24880

257 Samples

Download data: XLSX

(Submitter supplied) In Streptococcus pyogenes, mutation of GidA results in avirulence despite the same growth rate as the wild type. To understand the basis of this effect, global transcription profiling was conducted. Keywords: Wild type vs. GidA mutant Streptococcus pyogenes

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL4584

8 Samples

Download data: TIFF

(Submitter supplied) This study evaluate how catabolite control protein A (CcpA) inactivation affected gene transcript levels in the group A Streptococcus serotype M1 strain MGAS5005 during growth in standard laboratory medium. Keywords: transcriptome analysis

Organism:

Streptococcus pyogenes; Streptococcus sp. 'group A'

Type:

Expression profiling by array

Platform:

GPL6186

16 Samples

Download data: CEL

(Submitter supplied) Transcriptional profile of Streptococcus pyogenes stk mutant strain JRS2516 vs its wild type parent strain MGAS2221

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL10822

12 Samples

Download data: GPR

(Submitter supplied) We used RNA-Seq to assess how RocA regulates gene expression in stationary phase GAS cultures

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT

(Submitter supplied) We used RNA-Seq to assess how RocA regulates gene expression in stationary phase cultures of serotype M1 GAS

Organism:

Streptococcus pyogenes

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18784

4 Samples

Download data: TXT

(Submitter supplied) The goal of this study were to compare the transcriptoms of CcpA and CovR in group A Streptococcus. Isogenic mutant strains were created in which CcpA and CovR were inactivated alone and in combination. The transcriptomes of the four strains (wild-type, CcpA mutant, CovR mutant, and ccpA-covR double mutant) were then compared.

Organism:

Streptococcus sp. 'group A'; Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL9080

32 Samples

Download data: CEL

(Submitter supplied) The overall gene expression of a CvfA- mutant of Streptococcus pyogenes grown in C medium was compared to that of the wild type (HSC5spc). Stationary phase cells (8 hrs post-inoculation) were used because the gene expression of the CvfA- mutant was most different from that of the wild type at stationary phase, while the difference of gene expression between the CvfA- mutant and the wild type was negligible at exponential phase.

Organism:

Streptococcus pyogenes

Type:

Expression profiling by array

Platform:

GPL8518

6 Samples

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