GEO DataSets

(Submitter supplied) To test the contributions of phagocytic immune cells to mosquito immunity, we depleted mosquito phagocytes using clodronate liposomes and then challenged mosquitoes with malaria parasites. Observing a large increase in infection intensity, the purpose of the RNA-seq experiment is to determine their effects of phagocyte depletion on mosquito immunity.

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25232

8 Samples

Download data: TXT

(Submitter supplied) Background: The mosquito Anopheles gambiae is a major vector of human malaria. Increasing evidence indicates that blood cells (hemocytes) comprise an essential arm of the mosquito innate immune response against both bacteria and malaria parasites. To further characterize the role of hemocytes in mosquito immunity, we undertook the first genome-wide transcriptomic analyses of adult female An. gambiae hemocytes following infection by two species of bacteria and a malaria parasite. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platforms:

GPL8551 GPL8552 GPL8550

11 Samples

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(Submitter supplied) To examine the effects of 20E treatment on mosquito gene expression, treated mosquito cells were examined by RNA-seq to determine the influence of 20E treatment.

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25232

8 Samples

Download data: TXT

(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify transcripts that are specific or enriched in circulating hemocytes compared to either neuronal or to the rest of the body.

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

16 Samples

Download data: CEL, CHP

(Submitter supplied) Insect hemocytes mediate important cellular immune responses including phagocytosis and encapsulation, and also secrete immune factors such as opsonins, melanization factors, and antimicrobial peptides. In Anopheles, they contribute to the defense against malaria parasite invasion during the early sporogonic cycle. We used microarrays to identify if and to what degree circulating hemocytes have altered global expression profiles after infection with the rodent malaria parasite, Plasmodium berghei

Organism:

Plasmodium falciparum; Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL1321

6 Samples

Download data: CEL, CHP

(Submitter supplied) Transmission of malaria is dependent on the successful completion of the Plasmodium lifecycle in the Anopheles vector. Major obstacles are encountered in the midgut tissue, where most parasites are killed by the mosquito’s immune system. In the present study, DNA microarray analyses have been used to compare Anopheles gambiae responses to invasion of the midgut epithelium by the ookinete stage of the human pathogen Plasmodium falciparum and the rodent experimental model pathogen P. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by array

Platform:

GPL5001

24 Samples

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(Submitter supplied) Gene edited mosquitoes lacking a gamma-interferon-inducible lysosomal thiol reductase-like protein, namely (mosGILTnull) have decreased Plasmodium infection, which is linked to impaired ovarian development and immune activation. The transcriptome of A. gambiae mosGILTnull was therefore compared to wild type (WT) by RNA-sequencing to delineate mosGILT-dependent pathways. Compared to WT mosquitoes, mosGILTnull A. more...

Organism:

Anopheles gambiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32063

16 Samples

Download data: TXT