Similar studies for GEO DataSets (Select 200189306) - GEO DataSets

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Items: 20

Select item 2001893061.

Deficiency of the RNA-binding protein Cth2 extends yeast replicative lifespan by alleviating its repressive effects on mitochondrial function

(Submitter supplied) Iron dyshomeostasis contributes to aging, but little information is available about the molecular mechanisms. Here, we provide evidence that, in Saccharomyces cerevisiae, aging is associated with altered expression of genes involved in iron homeostasis. We further demonstrate that defects in the conserved mRNA-binding protein Cth2, which controls stability and translation of mRNAs encoding iron-containing proteins, increase lifespan by alleviating its repressive effects on mitochondrial function. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13821
15 Samples

Download data: TXT

Series

Accession:: GSE189306

ID:: 200189306

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Select item 2000540452.

Chemical potentiator of copper-accumulation and -toxicity: Probing iron- regulons of Saccharomyces cerevisiae

(Submitter supplied) RNA-seq was used to assess mRNA transcript abundance in wild type and fra2Δ S. cerevisiae (BY4741) cells treated with 2-(6-benzyl-2-pyridyl)quinazoline (BPQ) and CuSO4. BPQ potentiates copper toxicity and in yeast, in common with other organisms, a major cause of copper toxicity is damage of iron-sulphur clusters. Iron sensing within yeast relies on mitochondrial iron-sulphur cluster biosynthesis and therefore treatment with BPQ and copper can be used to mimic iron deficiency. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17342
18 Samples

Download data: TXT

Series

Accession:: GSE54045

ID:: 200054045

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Select item 2000448713.

Expression profile of Yeast Frataxin Mutants

(Submitter supplied) Yeast Frataxin Homologue 1 has been involved in oxidative stress and iron-sulfur biogenesis within the mitochondria. We have investigated the expression profile of conditional Yfh1 mutants. Yfh1 depletion leads to activation of iron uptake and repression

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL10039
6 Samples

Download data: GPR

Series

Accession:: GSE44871

ID:: 200044871

Select item 2000112364.

Cooperation of two mRNA-binding proteins drives metabolic adaptation to iron deficiency

(Submitter supplied) Expression of the yeast Cth2 protein stimulates degradation of mRNAs encoding proteins with Fe-dependent functions in metabolism, in iron storage and in other cellular processes. We demonstrate that in response to Fe deprivation, the Cth2-homologue, Cth1, stimulates specific degradation of mRNAs involved in mitochondrially localized activities that include respiration and amino acid biosynthesis. Furthermore, yeast cells grown under Fe deprivation accumulate mRNAs encoding proteins that function in glucose metabolism. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL90
9 Samples

Download data: CEL, CHP

Series

Accession:: GSE11236

ID:: 200011236

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Select item 2000851985.

CAN1 Arginine Permease Deficiency Extends Yeast Replicative Lifespan via Translational Activation of Stress Response Genes

(Submitter supplied) Transcriptional regulation plays an important role in the control of gene expression during aging. However, translation efficiency likely plays an equally important role in determining protein abundance, but has been relatively under studied in this context. Here we used RNA-seq and ribosome profiling to investigate the role of translational regulation in lifespan extension by CAN1 gene deletion in yeast. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13821
16 Samples

Download data: TXT

Series

Accession:: GSE85198

ID:: 200085198

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Select item 2000509976.

The evolutionary interplay between Puf RNA binding proteins and their RNA targets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Saccharomyces cerevisiae; Neurospora crassa; Neurospora crassa OR74A

Type:: Expression profiling by array

Platforms:: GPL14949 GPL10697
28 Samples

Download data: GPR

Series

Accession:: GSE50997

ID:: 200050997

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Select item 2000509967.

Identification of RNAs associated with S. cerevisiae or N. crassa Puf3

(Submitter supplied) Affinity purification of S. cerevisiae or N. crassa Puf3 from S. cerevisiae cells and identification of associated RNAs by microarray

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL10697
9 Samples

Download data: GPR

Series

Accession:: GSE50996

ID:: 200050996

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Select item 2000509958.

Gene expression profiling of Puf knockouts in N. crassa

(Submitter supplied) Gene expression profiling was performed to compare RNA abundances in mycelium of Puf knockout strains compared to that in mycelium of wild-type N. crassa

Organism:: Neurospora crassa; Neurospora crassa OR74A

Type:: Expression profiling by array

Platform:: GPL14949
19 Samples

Download data: GPR

Series

Accession:: GSE50995

ID:: 200050995

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Select item 2001278759.

Transcriptomic analysis of the iron depletion in yeast

(Submitter supplied) The goal of the project was to study the effects on transctiption and mRNA stability after iron depletion. We analyzed the effect of iron depletion caused by 100 μM of the Fe2+-specific chelator bathophenanthroline disulfonic acid (BPS) on the transcription rates, mRNA stabilities and mRNA levels by doing Genomic Run-On (GRO) experiments at 0, 10, 30, 90, 180 and 360 minutes after iron limitation.

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL13620
11 Samples

Download data: TXT

Series

Accession:: GSE127875

ID:: 200127875

Select item 20007236610.

Genome-wide mapping of decay factor-mRNA interactions in yeast identifies nutrient responsive transcripts as targets of the deadenylase Ccr4

(Submitter supplied) The Ccr4-Not complex is a major regulator of stress responses that controls gene expression at multiple levels, from transcription to mRNA decay. Ccr4, a core subunit of the complex, is the main cytoplasmic deadenylase in Saccharomyces cerevisiae, however its mRNA targets have not been mapped on a genome-wide scale. Here we describe a genome-wide approach, RNA immunoprecipitation-high throughput sequencing (RIP-seq), to identify the RNAs bound to Ccr4, and two proteins that associate with it, Dhh1 and Puf5. more...

Organism:: Saccharomyces cerevisiae

Type:: Other

Platform:: GPL15263
14 Samples

Download data: TXT

Series

Accession:: GSE72366

ID:: 200072366

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Select item 20015209511.

Rpb4 and Puf3

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Saccharomyces cerevisiae

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:: GPL13821 GPL19756
20 Samples

Download data: BEDGRAPH, BW

Series

Accession:: GSE152095

ID:: 200152095

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Select item 20015209412.

Rpb4 and Puf3 imprint and post-transcriptionally control stability of a common set of mRNAs in yeast

(Submitter supplied) Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, a crosstalk where mRNA decay machinery impacts transcription and transcription machinery influences mRNA stability. Rpb4, and likely the dimer Rpb4/7 seem to be the central components of the RNA pol II governing these processes. In this work we unravel more precisely the molecular mechanisms participated by Rpb4 that mediates the posttranscriptional events regulating mRNA imprinting and stability. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by high throughput sequencing

Platform:: GPL13821
4 Samples

Download data: BEDGRAPH

Series

Accession:: GSE152094

ID:: 200152094

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Select item 20015209313.

Potential modulation of Puf3 chromatin association mediated by Rpb4

(Submitter supplied) Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, a crosstalk where mRNA decay machinery impacts transcription and transcription machinery influences mRNA stability. Rpb4, and likely the dimer Rpb4/7 seem to be the central components of the RNA pol II governing these processes. Here we investigate the effect of ∆rrp4 on the potential chromatin association of the RNA binding protein Puf3 to chromatin in S. more...

Organism:: Saccharomyces cerevisiae

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL19756
16 Samples

Download data: BW

Series

Accession:: GSE152093

ID:: 200152093

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Select item 20008559114.

The Gcn4 Transcription Factor Reduces Protein Synthesis Capacity and Extends Yeast Lifespan

(Submitter supplied) In Saccharomyces cerevisiae, deletion of genes encoding proteins of the large ribosomal subunit (RPLs) increases the replicative lifespan in a Gcn4-dependent manner. However, how Gcn4, a key transcriptional activator of amino acid biosynthesis genes, increases lifespan, is unknown. Here we show that Gcn4 acts as a repressor of protein synthesis. By analyzing the mRNA and protein abundance, the ribosome occupancy and protein synthesis rate in various yeast strains, we demonstrate that Gcn4 is sufficient to reduce protein synthesis and to increase yeast lifespan. more...

Organism:: Saccharomyces cerevisiae

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing; Other

Platforms:: GPL17342 GPL19756
52 Samples

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Series

Accession:: GSE85591

ID:: 200085591

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Select item 20008559015.

The Gcn4 Transcription Factor Reduces Protein Synthesis Capacity and Extends Yeast Lifespan [RNA-Seq]

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by high throughput sequencing; Other

Platforms:: GPL17342 GPL19756
50 Samples

Download data: CSV, TXT

Series

Accession:: GSE85590

ID:: 200085590

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Select item 20008558816.

The Gcn4 Transcription Factor Reduces Protein Synthesis Capacity and Extends Yeast Lifespan [ChIP-Seq]

Organism:: Saccharomyces cerevisiae

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL17342
2 Samples

Download data: CSV

Series

Accession:: GSE85588

ID:: 200085588

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Select item 20007644417.

RNA-seq to identify P-body associated mRNAs upon glucose starvation, Na+ and Ca2+ stresses

(Submitter supplied) To examine mRNA species sequestered by P-bodies upon different stress conditions, we employed a modified crosslinking immunoprecipitation (cCLAP) technique to purify P-body associated mRNAs and analyzed by RNA-Seq. To compare, we also performed total RNA-Seq under the same stress conditions.

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL19756 GPL13821
32 Samples

Download data: TXT

Series

Accession:: GSE76444

ID:: 200076444

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Select item 20000939318.

Looking for mitochondrial associated RNA

(Submitter supplied) We investigated the peripheral mitochondrial localization of nuclear-encoded mRNAs (MLR) in various conditions in which translation was inhibited or the mRNA binding protein context altered (Delta puf3). We used cell fractionation protocols together with microarray to assess the distribution of mRNAs between free and mitochondrion-bound polysomes. Keywords: Mitochondrial-associated RNA localization in cells

Organism:: Saccharomyces cerevisiae

Type:: Other

Platform:: GPL6035
18 Samples

Download data: GPR, TIFF

Series

Accession:: GSE9393

ID:: 200009393

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Select item 20000439319.

Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast

(Submitter supplied) Abstract: Genes encoding RNA-binding proteins are diverse and abundant in eukaryotic genomes. Although some have been shown to have roles in post-transcriptional regulation of the expression of specific genes, few of these proteins have been studied systematically. We have used an affinity tag to isolate each of the five members of the Puf family of RNA-binding proteins in Saccharomyces cerevisiae and DNA microarrays to comprehensively identify the associated mRNAs. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platforms:: GPL3270 GPL3300 GPL3269
27 Samples

Download data

Series

Accession:: GSE4393

ID:: 200004393

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Select item 20000396920.

Puf3delta vs. wild type comparison

(Submitter supplied) Three independent experiments: S. cervisiae wild-type (BY4741) and Puf3 mutant cells were grown in minimal medium supplemented with 3% glycerol. Cells were harvested in mid-log phase by centrifugation and total RNA was prepared by hot-phenol extraction. cDNA was prepared with oligo-dT and using a mixture of amino-allyl dUTP and dNTPs, fluorescently labeled (Cy3= wild-type, Cy5 = mutant) and hybridize on S. more...

Organism:: Saccharomyces cerevisiae

Type:: Expression profiling by array

Platform:: GPL3300
3 Samples

Download data

Series

Accession:: GSE3969

ID:: 200003969

PubMed Full text in PMC Similar studies Analyze with GEO2R

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