GEO DataSets

(Submitter supplied) In this study, the methods to isolate and identify extracellular vesicles (EVs) including exosomes, from the seminal plasma (SP) of 3 fertile (F) and subfertile (S) bucks have been developed. Additionally, we investigated whether specific miRNA abundance differences between F and SF bucks could serve as fertility biomarkers. Ultracentrifugation and size exclusion chromatography analysis have made it possible to isolate different SP-EVs concentrations (8.53x10^11 ± 1.04x10^11 and 1.84x10^12 ± 1.75x10^11 particles/ml of SP; p=0,008), with a similar average size (143.9 ± 11.9 and 115.5 ± 2.4 nm; p=0.7422) in F and S males, respectively. more...

Organism:

Oryctolagus cuniculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26786

6 Samples

Download data: XLSX

(Submitter supplied) Objective: To study the uptake by human embryos of extracellular vesicles secreted by the maternal endometrium, and to investigate their miRNA cargo, in order to describe their role in implantation and early embryo development. Design: Prospective descriptive study. Subjects: Healthy women oocyte donors with confirmed fertility and day 5 human blastocysts. Intervention: Endometrial biopsies were collected from healthy oocyte donors undergoing transvaginal ultrasound-guided cyst aspiration for oocyte retrieval. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL21697

3 Samples

Download data: XLSX

(Submitter supplied) To study the impact of the obesity condition on circulating Extracellular Vesicle-miRNA expression signature in breast cancer patients, and identify potential obesity-related miRNAs that may be associated with progression.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL21697

12 Samples

Download data: TXT

(Submitter supplied) The continuing spread of HIV/AIDS is predominantly fueled by sexual exposure to HIV-contaminated semen. Seminal plasma (SP), the liquid portion of semen, harbors a variety of factors that may favor HIV transmission by facilitating viral entry into host cells, eliciting the production of pro-inflammatory cytokines, and enhancing the translocation of HIV across the genital epithelium. One important and abundant class of factors in SP is extracellular vesicles (EVs), which in general are important intercellular signal transducers. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11154

30 Samples

Download data: XLSX

(Submitter supplied) Exosomal RNA isolated from plasma was successfully profiled with a total of 65 immunology-related miNRAs in 13 mouse samples

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL25310

13 Samples

Download data: TXT

(Submitter supplied) Long-term side effects of doxorubicin include cardiotoxicity. Because studying transcriptional network alterations in human heart at early stages of cardiac dysfunction development is not feasible, in the present study, we used circulating microRNAs (miRNAs) to obtain insight into cellular processes in acute lymphoblastic leukemia (ALL) survivors with a history of doxorubicin treatment. We showed that altered miRNA profiles in plasma and circulating extracellular vesicles (EVs) and particularly the distribution of miRNAs between the two compartments in ALL survivors are linked to cellular transcriptomic processes controlled by transcription factors involved in epigenetic regulation, oxidative stress response, senescence, fibrosis, or epithelial-mesenchymal transition (EMT)—a phenomenon involved in organ injury, repair, and remodeling. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

240 Samples

Download data: TXT

(Submitter supplied) The role of extracellular vesicles (EVs), specifically exosomes, in intercellular communication likely plays a key role in placental orchestration of pregnancy and maternal immune sensing of the fetus. While murine models are powerful tools to study pregnancy and maternal-fetal immune interactions, in contrast to human placental exosomes, the content of murine placental and pregnancy exosomes remains largely understudied. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16417

12 Samples

Download data: TSV, TXT

(Submitter supplied) We report the miRNA profile of extracellular vesicles released from Atlantic salmon head kidney white blood cells that have been cultured for 1 day or 5 days.

Organism:

Salmo salar

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL27980

8 Samples

Download data: CSV

(Submitter supplied) Secreted microRNAs (miRNAs) enclosed within extracellular vesicles (EVs) play a pivotal role in intercellular communication by regulating recipient cell gene expression and affecting target cell function. Here, we report the isolation of three distinct EV subtypes from the human colon carcinoma cell line LIM1863--shed microvesicles (sMVs) and two exosome populations (immunoaffinity isolated A33-exosomes and EpCAM-exosomes). more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL22548

4 Samples

Download data: TXT

(Submitter supplied) In this study, we used RNA sequencing to provide a comprehensive overview of the expression profiles of small non-coding transcripts carried by the extracellular vesicles (EVs) derived from human adipose tissue stromal/stem cells (AT-MSCs) and human pluripotent stem cells (hPSCs), both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSC). Small non-coding RNA sequencing from EVs showed that the profile of miRNA expression in PSC follow the profile reported for cell derived miRNA; further, most abundant miRNAs were found to originate from specific miRNA families which are regulating pluripotency, reprograming and differentiation (miR-17–92, mir-200, miR-302/367, miR-371/373, CM19 microRNA cluster). more...

Organism:

Homo sapiens; other sequences

Type:

Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL24755 GPL18573

7 Samples

Download data: TXT

(Submitter supplied) Elevated ambient temperature during the summer season is a main cause of stress in dairy and beef cows, leading to impaired reproductive function and fertility. Follicular fluid extracellular vesicles (FF-EVs) play an important role in intrafollicular cellular communication by in part mediating the deleterious effects of heat stress (HS). Here we aimed to investigate the changes in FF-EV miRNA cargoes in beef cows in response to HS during the summer (SUM) compared to the winter (WIN) season using high throughput sequencing of FF-EV-coupled miRNAs.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26012

8 Samples

Download data: XLSX

(Submitter supplied) In our previous study, hsa-let-7d-3p, hsa-let-7e-5p,hsa-miR-146a-5p,hsa-miR-130a-3p, hsa-miR-151a-3p,were significantly upregulated in the plasma of atopic patients. To study the each function of let-7d-3p, let-7e-5p,miR-146a-5p,miR-130a-3p, miR-151a-3p which are significantly upregulated in the plasma of atopic patients, we performed mimic-transfected THP-1 cells, a mononuclear cell line, and performed comprehensive genetic analysis.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23159

6 Samples

Download data: CEL

(Submitter supplied) In our previous study, hsa-let-7d-5p,hsa-miR-27b-3p,hsa-miR-151-5p were significantly upregulated in the plasma of atopic patients. To study the each function of hsa-let-7d-5p,hsa-miR-27b-3p,hsa-miR-151-5p, which are significantly upregulated in the plasma of atopic patients, we performed mimic-transfected THP-1 cells, a mononuclear cell line, and performed comprehensive genetic analysis.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23159

4 Samples

Download data: CEL

(Submitter supplied) miRNAs are kown to be deeply involved in the progression of chronic inflammatory diseases. We comprehensively analyzed miRNAs in human plasma from AD(atopic dermatitis) and ACD(allergic conjunctival disease) patients. We performed a comprehensive human plasma miRNA analysis and identified significantly up-regulated miRNAs in both AD (atopic dermatitis) and ACD (allergic conjunctival disease).

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL21572

9 Samples

Download data: CEL, XLSX

(Submitter supplied) In our previous study, HAS-Let-7A-5P was significantly upregulated in the plasma of atopic patients. To study the function of hsa-let-7a-5p, which is significantly upregulated in the plasma of atopic patients, we performed mimic-transfected THP-1 cells, a mononuclear cell line, and performed comprehensive genetic analysis.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23159

2 Samples

Download data: CEL, XLSX

(Submitter supplied) Human saphenous vein perciytes (hSVPs) were proved increase angiogenesis in mouse myocardial infarction model by producing and releasing miR-132, which has proangiogenic, prosurvival, and antifibrotic activity. To investigate whether their exosomes have a role in their proangiogenic activity, we examined exosomes secreted by these cells. Nanoparticle tracking analysis (NTA) results indicated the presence of exosome size particles in the preparations. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL22600

3 Samples

Download data: TXT

(Submitter supplied) Pericardial sac surrounding the heart contains pericardial fluid (PF), which is rich in exosomes. PF exosomes increase angiogenesis in hypoxic endothelial cells and in animal model of hindlimb ischemia by passing the proangiogenic miRNAs to recipient cells. However, under pathological conditions such as diabetes, exosome cargo composition changes and harmful miRNAs can be transferred to the recipient cells and induce more deleterious effects in target tissues. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL22600

16 Samples

Download data: TXT, XLS

(Submitter supplied) P. aeruginosa were exposed to extracellular vesicles (EVs) secreted by primary human airway epithelial cells from 3 donors or PBS vehicle control for 6 h. Total RNA was isolated, small RNA libraries were prepared with the QIAseq smRNA kit (Qiagen) and 75-bp single-end reads were generated using Illumina MiniSeq. To assess transfer of human EV small RNAs to P. aeruginosa, sequences that did not map to the PA14 reference genome were aligned to the human genome.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30167

6 Samples

Download data: CSV

(Submitter supplied) Characterization of the small RNA content of extracellular vesicles isolated from cell culture supernatants of primary human airway epithelial cells from 3 donors.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

3 Samples

Download data: CSV

(Submitter supplied) SVF cells were isolated from liposuction material (hips, outer thigh) of healthy controls and lipedema patients and seeded for the collection of cell supernatants after 24 hours. After several centrifugation steps, the supernatants were concentrated and enriched for small extracellular vesicles. Total RNA was extracted from the concentrated supernatants and the small extracellular vesicles fraction. more...

Organism:

Homo sapiens

Type:

Other

Platform:

GPL27582

12 Samples

Download data: TXT