Similar studies for GEO DataSets (Select 200213209) - GEO DataSets

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Items: 20

Select item 2002132091.

Unannotated microprotein EMBOW switches WDR5 between epigenetic and mitotic roles during cell cycle

(Submitter supplied) The highly conserved WD40-repeat protein WDR5 is part of multiple functional complexes both inside and outside the nucleus, interacting with the MLL/SET1 histone methyltransferases that catalyze histone H3 lysine 4 (H3K4) di- and tri-methylation (me2,3), and KIF2A, a member of the Kinesin-13 family of microtubule depolymerase. It is currently unclear whether, and how, the distribution of WDR5 between complexes is regulated. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL24676
10 Samples

Download data: BED, BW

Series

Accession:: GSE213209

ID:: 200213209

PubMed Full text in PMC Similar studies

Select item 2002448992.

Mitotic gene regulation by the N-MYC-WDR5-PDPK1 nexus

(Submitter supplied) Background: During mitosis the cell depends on proper attachment and segregation of replicated chromosomes to generate two identical progeny. In cancers defined by overexpression or dysregulation of the MYC oncogene this process becomes impaired, leading to genomic instability and tumor evolution. Recently it was discovered that the chromatin regulator WDR5—a critical MYC cofactor—regulates expression of genes needed in mitosis through a direct interaction with the master kinase PDPK1. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24676
14 Samples

Download data: TXT

Series

Accession:: GSE244899

ID:: 200244899

PubMed Full text in PMC Similar studies Analyze with GEO2R

Select item 2002448353.

Mitotic gene regulation by the N-MYC-WDR5-PDPK1 nexus

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24676
4 Samples

Download data: TXT

Series

Accession:: GSE244835

ID:: 200244835

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Select item 2001504004.

The WDR5 WIN site interactome

(Submitter supplied) The WIN site of WDR5 is a druggable pocket that is crucial for WDR5 protein function and carries therapeutic potential for treating cancer. This study evaluates the protein interactions affected by small molecule blockade of the WIN site of WDR5. We find that PDPK1 directly binds the WIN site of WDR5, and we investigate this newfound interaction through proteomic, biochemical, and genomic methods.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL24676 GPL20301
20 Samples

Download data: TXT

Series

Accession:: GSE150400

ID:: 200150400

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Select item 2001364515.

WDR5 is a conserved regulator of protein synthesis gene expression

(Submitter supplied) WDR5 is a highly-conserved nuclear protein that performs multiple scaffolding functions in the context of chromatin. WDR5 is also a promising target for pharmacological inhibition in cancer, with small molecule inhibitors of an arginine-binding pocket of WDR5 (the "WIN" site) showing efficacy against a range of cancer cell lines in vitro. Efforts to understand WDR5, or establish the mechanism of action of WIN site inhibitors, however, are stymied by its many functions in the nucleus, and a lack of knowledge of the conserved gene networks—if any—that are under its control. more...

Organism:: Homo sapiens; Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

4 related Platforms
65 Samples

Download data: CSV, NARROWPEAK, TXT

Series

Accession:: GSE136451

ID:: 200136451

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Select item 2001451586.

Altered expression of genes in WDR5 inhibited (by OICR-9429) bladder cancer cells

(Submitter supplied) To explore the molecular mechanism underlying OICR-9429-induced WDR5 inhibition in BCa cells, a genome-wide RNA-sequencing was conducted to compare gene expression profiles between OICR-9429 treated T24, UM-UC-3 cells and their control cells

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24676
4 Samples

Download data: XLSX

Series

Accession:: GSE145158

ID:: 200145158

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Select item 2001785567.

Wdr5

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:: GPL24247
43 Samples

Download data: BED, BIGWIG, BW, GTF, NARROWPEAK

Series

Accession:: GSE178556

ID:: 200178556

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Select item 2001785558.

Transcriptome of Wdr5 and (or) p53 double knockout ESCs and embryoid bodies

(Submitter supplied) This study describes the transcriptome profiling of day 6 SFEBq embryonic bodies (EBs): 1) WT; 2) Wdr5 KO ; 3) Wdr5 KO with T12h hWDR5 rescue; 4) Wdr5 KO with T48h hWDR5 rescue

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24247
18 Samples

Download data: GTF

Series

Accession:: GSE178555

ID:: 200178555

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2001785549.

Genome profiling of MAX binding in mouse embryonic bodies using SFEBq differentiation methods

(Submitter supplied) This study describes the binding profile of MAX in mouse embryonic bodies at day 2 (WT, Wdr5 KO, Wdr5 and p53 double knockout)

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL24247
3 Samples

Download data: NARROWPEAK

Series

Accession:: GSE178554

ID:: 200178554

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Select item 20017855210.

Genome profiling of WDR5 and H3K4me3 binding in mouse embryonic stem cells

(Submitter supplied) This study describes the binding profile of WDR5 and H3K4me3 in Wdr5 and p53 double knockout ESC rescued with WDR5WT or WDR5S91KY191F

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL24247
6 Samples

Download data: BIGWIG, NARROWPEAK

Series

Accession:: GSE178552

ID:: 200178552

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Select item 20017855111.

Chromatin accessibility profiling of mouse embryonic stem cells and embryonic bodies using SFEBq differentiation methods upon Wdr5 and P53 deletion with or without WT or mutant hWDR5 rescue

(Submitter supplied) This study describes time-course chromatin accessibility profiling of mouse ESC and embryonic bodies n Wdr5 and p53 knockout (with or without WT or mutant hWDR5 rescue)

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL24247
16 Samples

Download data: BED, BW, NARROWPEAK

Series

Accession:: GSE178551

ID:: 200178551

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Select item 20011537712.

Displacement of WDR5 from chromatin by a pharmacological WIN site inhibitor with picomolar affinity

(Submitter supplied) We discovered potent small molecule inhibitors against the WIN site of WDR5. These inhibitors selectively block the proliferation of mammalian cells carrying fusions of the MLL1 oncogene. Here, we show that these inhibitors result in the rapid displacement of WDR5 from chromatin in both sensitive (MV4:11) and non-sensitive (K562) cell lines, induce early changes in the distribution of active polymerases at a subset of WDR5-bound genes, and induce global transcript changes that are consistent with induction of the tumor suppressor p53.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platforms:: GPL18573 GPL21290 GPL16791
77 Samples

Download data: NARROWPEAK, TXT

Series

Accession:: GSE115377

ID:: 200115377

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Select item 20012620713.

Interaction with WDR5 recruits MYC to a small cohort of genes required for tumor onset and maintenance

(Submitter supplied) The oncoprotein transcription factor MYC is overexpressed in the majority of human cancers. Key to its oncogenic activity is the ability of MYC to bind chromatin and regulate broad gene expression patterns that drive and maintain the tumorigenic state. The interaction of MYC with chromatin is absolutely dependent on interaction with MAX, but may also be facilitated by additional chromatin-resident proteins such as WDR5. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Other

Platforms:: GPL18573 GPL20301
42 Samples

Download data: NARROWPEAK, TXT

Series

Accession:: GSE126207

ID:: 200126207

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Select item 20017867714.

The WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platform:: GPL17021
12 Samples

Download data: BIGWIG, TXT

Series

Accession:: GSE178677

ID:: 200178677

PubMed Full text in PMC Similar studies

Select item 20017867615.

The WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape [RNA-seq]

(Submitter supplied) Two orthotopic pancreatic tumor mouse models were used for ChIP-Seq and RNA-Seq to identify genome-wide dysfunction of H3K4me3 and gene expression. Mouse pancreatic tumors have a genome-wide increase in H3K4me3 deposition as compared to normal pancreas. Osteopontin (OPN) and its receptor CD44 were identified being up-regulated in pancreatic tumors by their promoter H3K4me3 deposition. OPN protein is increased in both tumor cells and tumor-infiltrating immune cells in human pancreatic carcinoma and is inversely correlated with pancreatic cancer patient survival. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL17021
6 Samples

Download data: CSV, TXT

Series

Accession:: GSE178676

ID:: 200178676

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20017867516.

The WDR5-H3K4me3 epigenetic axis regulates OPN expression to compensate PD-L1 function to promote pancreatic cancer immune escape [ChIP-seq]

(Submitter supplied) Total RNA was isolated from normal C57BL/6 mouse pancreas, PANC02-H7, and UN-KC-6141 tumors from tumor-bearing mice. The RNA was used for RNA-Seq. The gene expression profiles between normal mouse pancreas and orthotopic pancreatic tumors were compared, and differentially expressed genes were identified.

Organism:: Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL17021
6 Samples

Download data: BIGWIG

Series

Accession:: GSE178675

ID:: 200178675

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20018378117.

WIN site inhibition disrupts a subset of WDR5 function

(Submitter supplied) This study demonstrates the impact of WIN site inhibitors versus WDR5 degradation on H3K4me and transcriptional processes in human Burkitt's lymphoma cells. We use RNA-seq to measure global transcript levels, ChIP-seq to map genomic H3K4me3, and PRO-seq to map genomic polymerase density and primary transcripts. Our data show that WIN site inhibition disables only a specific subset of WDR5 activity, and that H3K4me changes induced by WDR5 depletion do not explain accompanying transcriptional responses.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

Platform:: GPL24676
38 Samples

Download data: NARROWPEAK, TXT

Series

Accession:: GSE183781

ID:: 200183781

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Select item 20005527918.

A Role for WDR5 in Integrating Threonine 11 Phosphorylation to Lysine 4 Methylationon Histone H3 and in Prostate Cancer

(Submitter supplied) Upon androgen stimulation, PKN1-mediated histone H3 threonine 11 phosphorylation (H3T11P) promotes AR target genes activation. However, the underlying mechanism is not completely understood. Here, we show that WDR5, a subunit of the SET1/MLL complex, interacts with H3T11P and this interaction facilitates the recruitment of the SET1/MLL complex and subsequent H3K4 trimethylation (H3K4me3). Using ChIP-seq, we find that androgen stimulation results in a six-fold increase in the number of H3T11P-marked regions and induces WDR5 colocalization to one third of H3T11P-enriched promoters, thus establishing a genome-wide relationship between H3T11P and recruitment of WDR5. more...

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL11154
6 Samples

Download data: BEDGRAPH, TXT

Series

Accession:: GSE55279

ID:: 200055279

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 20019572919.

Discovery of a dual PROTAC for degrading WDR5 and Ikaros oncoproteins as therapeutics [GRO-Seq]

(Submitter supplied) WD repeat domain 5 (WDR5), a chromatin regulator associated with MLL complex and MYC oncoproteins, was shown to be critical for oncogenesis in human cancers and represents an attractive drug target. Inhibitors for targeting protein-protein interaction interfaces (PPIs) within WDR5 were developed; however, they inhibited only a part of WDR5-mediated functional interactions, exerting rather limited antitumor effects. more...