GEO DataSets

Analysis of blood monocytes differentiated to macrophages (MP) with CXC Chemokine Ligand 4 (CXCL4) and macrophage colony-stimulation factor (M-CSF). In atherosclerotic arteries, monocytes differentiate to MPs in the presence of growth factors, such as M-CSF, and chemokines, such as CXCL4.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL570

Series:

GSE20484

4 Samples

Download data: CEL

(Submitter supplied) Human blood monocytes were differentiated over six days with either 100 ng/ml M-CSF or 1 umol/l CXCL4 In atherosclerotic arteries, blood monocytes differentiate to macrophages in the presence of growth factors like macrophage colony-stimulation factor (MCSF) and chemokines like platelet factor 4 (CXCL4). To compare the gene expression signature of CXCL4-induced macrophages with MCSF-induced macrophages or macrophages polarized with IFN-γ/LPS (M1) or IL-4 (M2), we cultured primary human peripheral blood monocytes for six days. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3787

Platform:

GPL570

4 Samples

Download data: CEL

(Submitter supplied) Monocytes were induced to mature to macrophages with M-CSF. Cells were then activated with Interferon gamma and LPS or IL-4.

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS2429 GDS2430

Platforms:

GPL97 GPL96

30 Samples

Download data: CEL

Analysis of monocytes (MCs) treated with M-CSF to induce differentiation into macrophages (MPs), and mature MPs treated with either IFN-gamma and LPS or IL-4 to induce polarization to M1 or M2 cells, respectively. Results provide insight into MC to MP differentiation and polarized activation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 5 development stage sets

Platform:

GPL97

Series:

GSE5099

15 Samples

Download data: CEL

Analysis of monocytes (MCs) treated with M-CSF to induce differentiation into macrophages (MPs), and mature MPs treated with either IFN-gamma and LPS or IL-4 to induce polarization to M1 or M2 cells, respectively. Results provide insight into MC to MP differentiation and polarized activation.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 5 development stage sets

Platform:

GPL96

Series:

GSE5099

15 Samples

Download data: CEL

(Submitter supplied) Human cytomegalovirus induces a pro-inflammatory monocyte following infection. To begin to address how HCMV induces these rapid changes in infected monocytes, we examined the transcriptome of infected monocytes. Global transcriptional profiling using cDNA microarrays revealed a significant number of pro-inflammatory genes were upregulated within 4 hours post infection. Keywords: Disease State

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL8300

12 Samples

Download data: CEL, CHP

(Submitter supplied) Identification of genes differentially expressed between human monocyte-macrophages generated in the presence of either GM-CSF (termed M1) or M-CSF (termed M2)

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11010

6 Samples

Download data: TXT

(Submitter supplied) In the present study we aimed at producing homogeneous populations of monocytes and macrophages from human embryonic stem (hES) cells. Human embryonic stem cell lines KCL001, KCL002, and HUES-2 were differentiated into monocytes by coculture-free differentiation with two growth factors using a three-step method. The method involved embryoid body (EB) formation in hES media, directed differentiation with macrophage colony-stimulating factor and interleukin (IL)-3, and harvest of nonadherent monocytes from the culture supernatants. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

9 Samples

Download data: CEL

(Submitter supplied) Although the role of macrophage colony stimulating factor (M-CSF/CSF-1) in homeostasis and disease processes has been studied extensively in mice, little is known of the impact of this cytokine on differentiated human macrophages. Here we show that, in contrast to its effects on mouse bone marrow-derived macrophages (BMM), CSF-1 did not induce expression of urokinase plasminogen activator mRNA, repress expression of apolipoprotein E mRNA, or prime LPS-induced TNF secretion in human monocyte-derived macrophages (HMDM) from several independent donors. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3686

Platform:

GPL2507

6 Samples

Download data: TXT

Analysis of mature monocyte derived macrophages treated with colony stimulating factor 1 (CSF-1) in vitro for 6 hours. CSF-1 supports the proliferation and differentiation of monocytes and macrophages. Results provide insight into the effect of CSF-1 stimulation on mature monocytes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 agent, 3 individual sets

Platform:

GPL2507

Series:

GSE11061

6 Samples

Download data: TXT

(Submitter supplied) To examine mononuclear cell gene expression profiles in patients with and without SLE and subsets with and without atherosclerosis

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

72 Samples

Download data: TXT

(Submitter supplied) Analysis of the role of IL-10 on the transcriptional signature of LPS-activated human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 7 days in the presence of 1000 U/ml M-CSF, with cytokine addition every two days. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23227

6 Samples

Download data: TXT

(Submitter supplied) Analysis of the role of STAT3 on the transcriptional signature of LPS-activated human M-CSF-dependentmonocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures.monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells).monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 5 days in the presence of 1000 U/ml M-CSF, with cytokine addition every two days. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23227

12 Samples

Download data: TXT

(Submitter supplied) Analysis of the effect of various activation stimuli on the transcriptional signature of human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23227

63 Samples

Download data: TXT

(Submitter supplied) LPS treatment is considered as the paradigmatic stimulus for acquisition of the classical macrophage polarization/activation state. However, this definition does not take into account the influence that other macrophage differentiating/priming factors might have on the LPS response. In spite of the pathophysiological relevance of the response of M-CSF-dependent anti-inflammatory human macrophages (M-MØ) to endogenous and pathogenic stimuli, detailed analysis of the gene signature of activated anti-inflammatory M-CSF-dependent macrophages remained to be determined. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13497

12 Samples

Download data: TXT

(Submitter supplied) In atherosclerosis progression and regression, monocytes or monocyte-derived macrophages are the major immune cells in the plaque. It is important to understand the fate and characteristics of monocyte/macrophage during the plaque progression and regression. To characterize the fate of monocytes/macrophages, we performed single cell RNA sequencing of fate-mapped aortic CX3CR1-derived monocytes/macrophages from Cx3cr1CreERT2-IRES-YFP/+Rosa26floxed-tdTomato/+ mice with AAV-PCSK9 injection and fed a Western Diet. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21103

2 Samples

Download data: MTX, TSV

(Submitter supplied) Monocytes cultured in media containing GM-CSF and IL4 for 5 days normally differentiate into immature dendritic cells which, upon further stimulation with LPS for an additional 2 days, acquire a mature phenotype. In the present study, we evaluated the effect of human cord-blood mesenchymal stem cells (MSC) on human monocyte differentiation into immature and mature DCs by examining the trancriptional profile of the cells that were obtained after both the differentiation and maturation stages.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

18 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Other

Platforms:

GPL15456 GPL16791

52 Samples

Download data: BW, NARROWPEAK

(Submitter supplied) RNA modifications are essential for the establishment of cellular identity. Although increasing evidence indicates that RNA modifications control the innate immune response, their role in monocyte-to-macrophage differentiation and polarisation is unclear. We profile m6A epitranscriptomes of monocytes and macrophages at resting, pro- and anti-inflammatory states.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL16791

16 Samples

Download data: BW, NARROWPEAK

(Submitter supplied) RNA modifications are essential for the establishment of cellular identity. Although increasing evidence indicates that RNA modifications control the innate immune response, their role in monocyte-to-macrophage differentiation and polarisation is unclear. We profile translatomes of monocytes and macrophages at resting, pro- and anti-inflammatory states.

Organism:

Homo sapiens

Type:

Other

Platform:

GPL16791

12 Samples

Download data: BW, TXT