GEO DataSets

(Submitter supplied) NTHi infection of Normal Human Nasal Epithelia (NHNE) for 14 days

Organism:

Haemophilus influenzae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL25730

28 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Haemophilus influenzae; Porphyromonas gingivalis W83; Porphyromonas gingivalis

Type:

Expression profiling by array; Expression profiling by high throughput sequencing

Platforms:

GPL33075 GPL18755

24 Samples

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(Submitter supplied) To understand the role of CdhR and its adjacent gene PG1236 in nitric oxide (NO) stress resistance, isogenic mutants P. gingivalis FLL457 (ΔPG1237::ermF), FLL458 (ΔPG1236::ermF) and FLL459 (ΔPG1236-37::ermF) were made by allelic exchange mutagenesis and their gene expression was studied under control and NO stress conditions. DNA microarray analysis of FLL457 showed that approximately 2% of the genes were up regulated and over 1% of the genes down regulated. more...

Organism:

Haemophilus influenzae; Porphyromonas gingivalis W83

Type:

Expression profiling by array

Platform:

GPL18755

12 Samples

Download data: XLS

(Submitter supplied) Nontypeable Haemophilus influenzae (NTHi) is a pathobiont which chronically colonises the airway of individuals with chronic respiratory disease. It is unclear how NTHi persists in the airway, however accumulating evidence suggests that NTHi can invade and persist within macrophages. To better understand the mechanisms of NTHi persistence within macrophages, this work developed an in vitro model of NTHi intracellular persistence using human monocyte-derived macrophages (MDM). more...

Organism:

Haemophilus influenzae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24676 GPL30396

20 Samples

Download data: TXT

(Submitter supplied) Pathogenic bacteria encounter a variety of stressful host environments during infection. Their responses to meet these challenges protect them from deadly damages and aid in adaption to harmful environments. Bacterial products critical for this protection are therefore interesting as suitable targets for new antimicrobials. To shed light on the complex array of molecular pathways involved in bacterial stress responses we challenged 32 diverse human pathogenic bacteria to 11 infection related stress conditions and catalogued their transcriptomes. more...

Organism:

Helicobacter pylori; Enterococcus faecalis; Neisseria meningitidis; Staphylococcus epidermidis; Streptococcus pyogenes; Listeria monocytogenes; Salmonella enterica; Achromobacter xylosoxidans; Borreliella burgdorferi; Pseudomonas aeruginosa; Legionella pneumophila; Klebsiella pneumoniae; Yersinia pseudotuberculosis; Vibrio cholerae; Streptococcus suis; Streptococcus agalactiae; Streptococcus pneumoniae; Mycobacterium tuberculosis; Burkholderia pseudomallei; Campylobacter jejuni; Francisella tularensis; Acinetobacter baumannii; Neisseria gonorrhoeae; Escherichia coli; Shigella flexneri; Aggregatibacter actinomycetemcomitans; Haemophilus influenzae; Staphylococcus aureus subsp. aureus MRSA252; Staphylococcus aureus subsp. aureus MSSA476

Type:

Expression profiling by high throughput sequencing

30 related Platforms

1122 Samples

Download data: TXT

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain 723 strains expressing methyltransferase ModA2 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19560

6 Samples

Download data: TXT

(Submitter supplied) We used an in vitro evolved strain of nontypeable Haemophilus influenzae (NTHI) to examine the metabolic contributions to persistence RNASeq analysis identified 55 transcripts that significantly changed in amount within in vitro biofilms formed by RM33, as compared to the parental strain. Expression of genes encoding all enzymes within the tryptophan and glycogen pathways was significantly increased in biofilms formed by RM33 and the parental strains. more...

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19560

8 Samples

Download data: TXT

(Submitter supplied) Haemophilus influenzae responds to the steroid beclomethasone used in asthma therapy by modulation of biofilm formation and antibiotic tolerance

Organism:

Haemophilus influenzae Rd KW20

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26079

12 Samples

Download data: XLSX

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain R2866 expressing methyltransferase ModA10 (ON) or not (LOCK_OFF).

Organism:

Haemophilus influenzae R2866

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26504

6 Samples

Download data: TXT

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain 84P36H1 strains expressing methyltransferase ModA18 (ON) or not (OFF).

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) Comparison of RNA expression profiles from NTHi strain 10P129H1 strains expressing methyltransferase ModA15 (ON) or not (OFF).

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

18 Samples

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(Submitter supplied) comparison of RNA expression profiles from NTHi strain 1209 strains expressing methyltransferase ModA9 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) comparison of RNA expression profiles from NTHi strain 477 strains expressing methyltransferase ModA5 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) comparison of RNA expression profiles from NTHi strain C486 strains expressing methyltransferase ModA4 (ON) or not (OFF)

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25730

6 Samples

Download data: TXT

(Submitter supplied) Characterization of host-pathogen interactions is critical for the development of next-generation therapies and vaccines. Classical approaches involve the use of transformed cell lines and/or animal models which may not reflect the complexity and response of the human host. We reconstituted the ciliated human bronchial epithelium in vitro using primary bronchial epithelial cells to simultaneously monitor the infection-linked global changes in nontypeable Haemophilus influenzae (NTHi) and infected host epithelia gene expression by dual RNA-seq. more...

Organism:

Haemophilus influenzae; Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL16791 GPL19560

24 Samples

Download data: TXT

(Submitter supplied) H. influenzae R2866 contains a phase-variable DNA methylase modA10, by virtue of 16 tetrameric repeats present after the start codon. Gain or loss of repeats during replication by slipped-strand mispairing can result in a non-functional reading frame. Microarray analyses of wild-type and insertionally inactivated modA were performed to determine genes affected by phase variable modification.

Organism:

Haemophilus influenzae; Haemophilus influenzae R2866

Type:

Expression profiling by array

Platform:

GPL6594

10 Samples

Download data: XLSX

(Submitter supplied) Transcriptome analysis of NTHi 86-028NPrpsL, NTHi 86-028NPrpsL∆fur, and NTHi 86-028NPrpsL∆fur(pT-fur) strains Nontypeable Haemophilus influenzae (NTHi) is a commensal microorganism of the normal human nasopharyngeal flora, yet also an opportunistic pathogen of the upper and lower respiratory tracts. Changes in gene expression patterns in response to host microenvironments are likely critical for survival. more...

Organism:

Haemophilus influenzae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18879

9 Samples

Download data: XLSX

(Submitter supplied) Investigation of whole genome gene expression differences between a full (four-gene) SlrV locus 1 deletion compared to the WT PittII strain. Removal of the four SlrV genes (aka macrophage survival factors : Δmsf) results in a competetive defect with the WT strain in in vitro biofilms and in the otitis media chinchilla model. It also results in an inability to persist within macrophages long-term in vitro and to escape from the middle-ear space and infect other sites in the chinchilla model.

Organism:

Haemophilus influenzae PittII; Haemophilus influenzae

Type:

Expression profiling by array

Platform:

GPL16197

8 Samples

Download data: PAIR

(Submitter supplied) Non-typeable Haemophilus influenzae (NTHi) contains an N6-adenine DNA-methyltransferase (ModA), that is subject to phase variable expression (random ON/OFF switching). Five modA alleles, modA2, 4, 5, 9 and 10, account for over two-thirds of clinical otitis media isolates surveyed. Single Molecule Real Time (SMRT) methylome analysis identified the DNA recognition motifs for all five of these modA alleles. more...

Organism:

Haemophilus influenzae

Type:

Expression profiling by array

Platform:

GPL20324

4 Samples

Download data: TXT