GEO DataSets

(Submitter supplied) To identify the role of the endolysosomal (EL) protein network in atrial fibrillation (AF), we applied a newly developed organelle isolation method to the AF goat model. To accomplish the goal, we used proteomics, transcriptomics, integrated analysis, enzyme assays, electron tomography, and western blotting and identified several regulating EL proteins, related protein networks and pathways.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

8 Samples

Download data: TXT

(Submitter supplied) Mammary epithelial cells (MECs), the only cell type which could be capable of secreting milk in mammary gland, can be applied for the generation of transgenic mammary cell/gland bioreactor. However, continuously achieving large amount of high-quality goat MECs by non-invasion method, especially prepuberal MECs, in vitro has been a challenge. Here, we show that a small molecules cocktail enables the in vitro generation of chemically-induced mammary epithelial cells (CiMECs) with milk-secreting properties from goat ear fibroblasts. more...

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL21299

6 Samples

Download data: TXT

(Submitter supplied) To reveal the transcriptomic changes of hypothalamic tissue in goats from birth to sexual maturity, we performed transcriptome sequencing of Jining Grey Goat hypothalamic tissues at four developmental stages after birth. A total of 20 libraries from four different development stages (5 goats per stage) were successfully constructed, and the corresponding mRNA and lncRNA expression profile were obtained.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

20 Samples

Download data: TXT

(Submitter supplied) Molecular mechanisms of follicular atresia and prolificacy of mammal remain unclear. We surveyed the single-cell transcriptomic landscape of ovaries from single and prolificacy goat and identified seven ovarian cell types with distinct gene-expression, transcriptional factor networks and reciprocal interactions signatures. In-depth dissection of gene-expression dynamics of granulosa cells (GCs) that displayed development stage-specific expression patterns and specific gene signatures were identified that may reflect developmental competency and ovarian reserve. more...

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

4 Samples

Download data: MTX, TSV, XLS, XLSX

(Submitter supplied) Ovarian and uterine tissues from five female Jining Gray goats were collected at the following time points: after birth (D1), 2-month-old (M2), 4-month-old (M4), and 6-month-old (M6). All samples were applied for rRNA-depleted RNA-seq libraries construction and transcriptome sequencing. We investigated the transcriptomic mechanisms involved in reproductive regulation in the ovary and uterus during sexual maturation.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

40 Samples

Download data: XLSX

(Submitter supplied) Ovarian and uterine tissues from five female Jining Gray goats were collected at the following time points: after birth (D1), 2-month-old (M2), 4-month-old (M4), and 6-month-old (M6). All samples were utilized for the construction of small RNA-seq libraries and subsequent small RNA sequencing. We explored the role of small RNAs in regulating reproductive processes in the ovary and uterus during sexual maturation.

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL27106

40 Samples

Download data: TXT

(Submitter supplied) We found piRNAs with different lengths represented the predominant small RNA species in oocytes from the 12 explored species, except mouse. We found endo-siRNAs resulted from the truncated Dicer isoform were mouse-specific, and os-piRNAs associating with PIWIL3 in human oocytes are widespread in mammals and are typically with low levels of the 2’-3’-O-methylation. The sequences of many highly expressed piRNA clusters are fast-evolving compared with their syntenic genomic locations, and the TE families distributing in the conserved piRNA clusters are various between species.

Organism:

Macaca fascicularis; Oryctolagus cuniculus; Cricetulus griseus; Mesocricetus auratus; Mus musculus; Capra hircus; Rattus norvegicus; Danio rerio; Homo sapiens; Canis lupus familiaris; Sus scrofa domesticus; Cavia porcellus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Other

12 related Platforms

138 Samples

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(Submitter supplied) We measued IgG autoantibodies associated with Connective Tissue Diseases (CTDs) and Anti-Cytokine Antibodies (ACA) in SLE and healthy controls who received the BNT162b2 vaccine.

Organism:

Triticum aestivum; Capra hircus; Homo sapiens; Sus scrofa; Escherichia coli; Spodoptera frugiperda; Bos taurus

Type:

Protein profiling by protein array

Platform:

GPL34257

102 Samples

Download data: TXT, XLSX

(Submitter supplied) To describe the gene expression profile of brain and muscle across ruminants, we performed gene expression analysis of brain and muscle using RNA-seq data for three ruminants.

Organism:

Bos taurus; Capra hircus; Ovis aries

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL24230 GPL23810 GPL25639

18 Samples

Download data: TXT

(Submitter supplied) This study is the first to report a genome-wide comparative DNA methylation map in three ruminants using WGBS technology.

Organism:

Ovis aries; Bos taurus; Capra hircus

Type:

Methylation profiling by high throughput sequencing

Platforms:

GPL24230 GPL25639 GPL23810

24 Samples

Download data: TXT

(Submitter supplied) To explore functional circRNAs during goat muscle development, we systematically investigated the circRNAs profiles using high throughput transcriptome sequencing technology (RNA-seq) at key developmental stages of fetus and Kid in Haimen goat.

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL27106

6 Samples

Download data: XLSX

(Submitter supplied) The difference in liver cell types from healthy, non-alcoholic steatohepatitis (NASH) and non-alcoholic steatohepatitis tolerance goats.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

3 Samples

Download data: MTX, TSV

(Submitter supplied) Combining RNA-seq with ATAC-seq, we screened the open chromatin of dairy goat mammary epithelial cells during lactation and then integrated human lysozyme into various open regions using the CRISPR/Cas9 system. A comparison of lysozyme gene expression and integration efficiency showed that chromatin accessibility at target sites was significantly positively correlated with gene insertion efficiency, and the insertion of foreign genes into highly accessible chromatin regions could enhance its expression.

Organism:

Capra hircus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL33145

6 Samples

Download data: BW, TXT

(Submitter supplied) Cows were fed a lactation diet at ad libitum intake (n = 6). At 27±3 days in milk, cows were injected with 50 µg of LPS E. coli in one healthy rear mammary quarter. Milk samples were collected just before LPS challenge (LPS-) and 6.5 h after LPS challenge (LPS+) from the same cows. Microarray analysis was performed using customized 8x60K ruminant miRNA microarrays to compare LPS- to LPS+ miRNome. MiRNome comparison between LPS- and LPS+ identified 37 differentially abundant miRNAs (q-value ≤ 0.05)

Organism:

Bos taurus; Capra hircus; Ovis aries

Type:

Non-coding RNA profiling by array

Platform:

GPL33332

12 Samples

Download data: TXT

(Submitter supplied) As goats are important domestic animals, the regulation of germline and gonadal somatic cells in goats during the mitotic quiescence phase has not been investigated previously. In this study, we employed single-cell RNA sequencing to identify transcriptional signatures of major prospermatogonia and somatic cell types of the testes in goats from E85, E105 and E125.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27106

3 Samples

Download data: MTX, TSV

(Submitter supplied) The mammary gland redeveloped to the pre-pregnancy state during involution, which shows that the mammary cells have the characteristics of remodeling. The rapidity and degree of mammary gland involution are different between mice and dairy livestock (dairy cows and dairy goats). However, the molecular genetic mechanism of miRNA in involution and remodeling of goat mammary gland has not yet been clarified. more...

Organism:

Capra hircus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19149

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus; Capra hircus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL27106 GPL24247

8 Samples

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(Submitter supplied) In order to explore the mechanisms of Dmrt1 in regulating testicular immune homeostasis, we performed ChIP-seq analysis on primary SCs infected with a lentiviral vector expressing FLAG-tagged Dmrt1

Organism:

Capra hircus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL27106

2 Samples

Download data: TXT

(Submitter supplied) Emerging data has highlighted the importance of short-chain fatty acids (SCFAs) on ruminal microbiome and derived metabolism profiling, and ruminal epithelial health and nutritional absorption in ruminants. However, little is known about the roles of SCFAs on ileal microbiome profiles. Here, we combined infusion of three SCFAs, to study their different roles in ileal microbiome succession profiling using a in vivo goat model.

Organism:

Capra hircus

Type:

Other

Platform:

GPL32968

12 Samples

Download data: FASTA, XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Capra hircus

Type:

Expression profiling by high throughput sequencing; Other

Platforms:

GPL32967 GPL32968

36 Samples

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