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| Status |
Public on May 19, 2014 |
| Title |
Evidence that variation in adult body size among mammalian species is achieved by modulating the pace of a growth-regulating genetic program |
| Platform organism |
Bos taurus |
| Sample organism |
Ovis aries |
| Experiment type |
Expression profiling by array
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| Summary |
Body size varies enormously among mammalian species. In small mammals, body growth is typically suppressed rapidly, within weeks, whereas in large mammals, growth is suppressed slowly, over years, allowing for a greater adult size. We recently reported evidence that body growth suppression in rodents is caused in part by a juvenile genetic program that occurs in multiple tissues simultaneously and involves the downregulation of a large set of growth-promoting genes. We hypothesized that this genetic program is conserved in large mammals but that its time course is evolutionarily modulated such that it plays out more slowly, allowing for more prolonged growth. Consistent with this hypothesis, using expression microarray analysis, we identified a set of genes that are downregulated with age in both juvenile sheep kidney and lung. This overlapping gene set was enriched for genes involved in cell proliferation and growth and showed striking similarity to a set of genes downregulated with age in multiple organs of the juvenile mouse and rat, indicating that the multiorgan juvenile genetic program previously described in rodents has been conserved in the 80 million years since sheep and rodents diverged in evolution. Using microarray and real-time PCR, we found that the pace of this program was most rapid in mice, more gradual in rats, and most gradual in sheep. The findings support the hypothesis that a growth-regulating genetic program is conserved among mammalian species but that its pace is modulated to allow more prolonged growth and therefore greater adult body size in larger mammals.
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| Overall design |
Tissues samples were collect from Lung and Kindey respectively with five biological replications of sheep (Ovis aries) that were collected, frozen in liquid nitrogen, and stored at -70 °C and tested on bovine array.
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| Contributor(s) |
Delaney A, Lui JK, Rezvani G, Chen W, Forcinito P, Cheung CF, Padmanabhan V, Baron J |
| Citation(s) |
24776848 |
| Submission date |
Jul 16, 2013 |
| Last update date |
May 19, 2014 |
| Contact name |
WeiPing Chen |
| E-mail(s) |
weipingChen@niddk.nih.gov
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| Phone |
301-496-0175
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| Organization name |
NIDDK/NIH
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| Department |
GCL
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| Lab |
Genomics Core Lab
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| Street address |
Bldg 8, Room 1A11, NIDDK/NIH
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL2112 |
[Bovine] Affymetrix Bovine Genome Array |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA212309 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE48916_RAW.tar |
223.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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