GEO DataSets

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by high throughput sequencing; Genome binding/occupancy profiling by genome tiling array; Genome variation profiling by genome tiling array; Expression profiling by high throughput sequencing

4 related Platforms

16 Samples

Download data: BED, TXT, WIG

(Submitter supplied) Comparative genomic hybridization was performed to compare levels of gDNA in third instar salivary glands of Drosophila mutants/nulls in the SuUR and orc proteins, compared with 0-2hr diploid embryo gDNA. This illustrates regions of differential replication in the genome.

Organism:

Drosophila melanogaster

Type:

Genome variation profiling by genome tiling array

Platforms:

GPL11290 GPL7787

5 Samples

Download data: TXT, WIG

(Submitter supplied) ChIP was performed to identify regions of gDNA bound by orc2 in third instar salivary glands of Drosophila WT and SuUR mutants. This demonstrated that ORC does not localize to regions that are under-replicated in SuUR mutant third instar salivary glands.

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL11290 GPL11023 GPL7787

4 Samples

Download data: TXT, WIG

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array; Expression profiling by high throughput sequencing

4 related Platforms

8 Samples

Download data: NDF, TXT, WIG

(Submitter supplied) Chromatin immunoprecipitation of the Origin Recognition Complex (ORC) followed by hybridization to genome-wide tiling microarrays demonstrated that ORC localizes to the six Drosophila amplicons in follicle cells (DAFC) as well as other non-amplified loci

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by genome tiling array

Platforms:

GPL7787 GPL11023

3 Samples

Download data: NDF, TXT, WIG

(Submitter supplied) modENCODE_submission_3441 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication initiation complexes. These experiments will be conducted in multiple cell types and developmental tissues. more...

Organism:

Drosophila melanogaster

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL7787

10 Samples

Download data: GFF3, TXT

(Submitter supplied) DNA copy number profile of Drosophila Kc167 cells comparing control pUC dsRNA treated cells and geminin dsRNA treated cells.

Organism:

Drosophila melanogaster

Type:

Genome variation profiling by genome tiling array

Platforms:

GPL7787 GPL7788

3 Samples

Download data: BED, TXT

(Submitter supplied) modENCODE_submission_725 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Analysis of copy number variation across modENCODE Drosophila cell line by comparative genomic hybridization (CGH). We observe numerous copy number variations for the different cell lines, with each cell line having a distinct ploidy signature. more...

Organism:

Drosophila melanogaster

Type:

Genome variation profiling by genome tiling array

Platform:

GPL7787

2 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) modENCODE_submission_723 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Most terminally differentiated Drosophila tissues are either polyploid or polytene. Unlike normal chromosomes, where the entire chromosome must be replicated exactly once, polytene chromosomes are often differentially replicated with many regions underreplicated and some overreplicated. more...

Organism:

Drosophila melanogaster

Type:

Genome variation profiling by genome tiling array

Platform:

GPL7787

2 Samples

Download data: GFF, TXT, WIG

(Submitter supplied) modENCODE_submission_720 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Most terminally differentiated Drosophila tissues are either polyploid or polytene. Unlike normal chromosomes, where the entire chromosome must be replicated exactly once, polytene chromosomes are often differentially replicated with many regions underreplicated and some overreplicated. more...

Organism:

Drosophila melanogaster

Type:

Genome variation profiling by genome tiling array

Platform:

GPL7787

1 Sample

Download data: GFF, TXT, WIG

(Submitter supplied) modENCODE_submission_711 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Early origins of replication were identified by treating cells with hydroxyurea (HU), a potent inhibitor of nucleotide synthesis, in the presence of the nucleotide analogue BrdU. Treatment of synchronized ML-DmBG3-c2 cells with HU stalls replication forks and activates the intra S-phase checkpoint, thereby limiting BrdU incorporation to those sequences mmediately adjacent to early activating replication origins. more...

Organism:

Drosophila melanogaster

Type:

Other

Platform:

GPL7787

3 Samples

Download data: GFF, TXT, WIG

(Submitter supplied) modENCODE_submission_710 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Early origins of replication were identified by treating cells with hydroxyurea (HU), a potent inhibitor of nucleotide synthesis, in the presence of the nucleotide analogue BrdU. Treatment of synchronized S2-DRSC cells with HU stalls replication forks and activates the intra S-phase checkpoint, thereby limiting BrdU incorporation to those sequences immediately adjacent to early activating replication origins. more...

Organism:

Drosophila melanogaster

Type:

Other

Platform:

GPL7787

3 Samples

Download data: GFF, TXT, WIG

(Submitter supplied) modENCODE_submission_709 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: Early origins of replication were identified by treating cells with hydroxyurea (HU), a potent inhibitor of nucleotide synthesis, in the presence of the nucleotide analogue BrdU. Treatment of synchronized Kc167 cells with HU stalls replication forks and activates the intra S-phase checkpoint, thereby limiting BrdU incorporation to those sequences immediately adjacent to early activating replication origins. more...

Organism:

Drosophila melanogaster

Type:

Other

Platform:

GPL7787

4 Samples

Download data: GFF, TXT, WIG

(Submitter supplied) modENCODE_submission_670 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The relative time of replication for all unique sequences in the Drosophila genome was determined by synchronizing tissue culture cell and differentially labeling early and late replicating intermediates. The differentially labeled replication intermediates were then hybridized to Agilent genomic tiling arrays to identify early and late replicating domains. more...

Organism:

Drosophila melanogaster

Type:

Other

Platform:

GPL7787

3 Samples

Download data: TXT

(Submitter supplied) modENCODE_submission_669 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The relative time of replication for all unique sequences in the Drosophila genome was determined by synchronizing tissue culture cell and differentially labeling early and late replicating intermediates. The differentially labeled replication intermediates were then hybridized to Agilent genomic tiling arrays to identify early and late replicating domains. more...

Organism:

Drosophila melanogaster

Type:

Other

Platform:

GPL7787

3 Samples

Download data: TXT

(Submitter supplied) modENCODE_submission_668 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The relative time of replication for all unique sequences in the Drosophila genome was determined by synchronizing tissue culture cell and differentially labeling early and late replicating intermediates. The differentially labeled replication intermediates were then hybridized to Agilent genomic tiling arrays to identify early and late replicating domains. more...

Organism:

Drosophila melanogaster

Type:

Other

Platform:

GPL7787

3 Samples

Download data: TXT