GEO DataSets

(Submitter supplied) The Saccharomyces cerevisiae Yap1p transcription factor is required for the H2O2-dependent activation of many antioxidant genes including the TRX2 gene encoding thioredoxin 2. To identify factors that regulate Yap1p activity, we carried out a genetic screen for mutants that show elevated expression of a TRX2-HIS3 fusion in the absence of H2O2. Two independent mutants isolated in this screen carried mutations in the TRR1 gene encoding thioredoxin reductase. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2659 GPL2658 GPL2657

15 Samples

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(Submitter supplied) Fcp1 is a protein phosphatase that facilitates transcription elongation and termination by dephosphorylating the C-terminal domain of RNA Polymerase II. Here, we report an unanticipated role in the repression of the oxidative stress induced genes. High-throughput genetic and gene expression profiling of FCP1 mutants uncovered a link to Skn7, a key transcription factor in the oxidative stress response pathways, as well as a link to the Mediator complex kinase subunit, Cdk8. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL11232

16 Samples

Download data: TXT

(Submitter supplied) Oxidative stress is a harmful condition in a cell, tissue, or organ, caused by an imbalnace between reactive oxygen species and other oxidants and the capacity of antioxidant defense systems to remove them. The budding yeast S. cerevisiae has been the major eukaryotic model for studies of response to oxidative stress. We used microarrays to study the genome-wide temporal response of the yeast S. cerevisiae to oxidative stress induced by cumene hydroperoxide. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS3035

Platform:

GPL90

48 Samples

Download data: CEL

Analysis of Saccharomyces cerevisiae cultures at various time points up to 120 minutes following treatment with the oxidant cumene hydroperoxide (CHP). Results provide insight into molecular mechanisms underlying the response of the yeast S. cerevisiae to oxidative stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, transformed count, 2 agent, 8 time sets

Platform:

GPL90

Series:

GSE7645

48 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13821

66 Samples

Download data: WIG

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13821

30 Samples

Download data: WIG

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13821

18 Samples

Download data: WIG

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...

Organism:

Saccharomyces cerevisiae

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13821

18 Samples

Download data: WIG

(Submitter supplied) We explored genomic expression patterns in the yeast Saccharomyces cerevisiae responding to diverse environmental transitions. DNA microarrays were used to measure changes in transcript levels over time for almost every yeast gene, as cells responded to temperature shocks, hydrogen peroxide, the superoxide-generating drug menadione, the sulfhydryl-oxidizing agent diamide, the disulfide-reducing agent dithiothreitol, hyper- and hypo-osmotic shock, amino acid starvation, nitrogen source depletion, and progression into stationary phase. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Datasets:

GDS15 GDS16 GDS17 GDS18 GDS19 GDS20 GDS21 GDS30 GDS31 GDS33 GDS34 GDS35 GDS36 GDS108 GDS111 GDS112 GDS113 GDS115

9 related Platforms

156 Samples

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Cells grown in minimal medium lacking amino acids and adenine. Samples taken at 0, 0.5, 1, 2, 4 and 6 hours. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL54

Series:

GSE18

5 Samples

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2.5 mM dithiothrietol (DTT) added to culture and samples collected at several time points up to 480 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 7 time sets

Platform:

GPL54

Series:

GSE18

7 Samples

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Cultures shifted from 30°C to 37°C and samples collected at 0, 5, 15, 30 and 60 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL54

Series:

GSE18

5 Samples

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Samples collected from cells grown continuously at 15°C, 17°C, 21°C, 25°C, 29°C, 33°C and 36°C. Included as part of a detailed characterization of global expression programs triggered by stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 7 temperature sets

Platform:

GPL54

Series:

GSE18

8 Samples

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Superoxide-generating drug menadione bisulphate (1 mM) added to culture and samples removed at several time points up to 160 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 9 time sets

Platform:

GPL64

Series:

GSE18

9 Samples

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Heat shock from 29°C to 33°C. Samples collected at 5, 15, 30 and 90 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 4 time sets

Platform:

GPL56

Series:

GSE18

4 Samples

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Heat shock from 29°C to 33°C, with or without addition of 1 M sorbitol. Samples collected at 5, 15 and 30 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 2 agent, 3 time sets

Platform:

GPL56

Series:

GSE18

6 Samples

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Heat shock from 37°C to 25°C. Samples collected at 15, 30, 45, 60 and 90 minutes. Included as part of a detailed characterization of global expression programs triggered by environmental stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL56

Series:

GSE18

5 Samples

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Cells resuspended in YPD medium without sorbitol. Samples recovered at 5, 15, 30, 45 and 60 minutes. Included as part of a detailed characterization of global expression programs triggered by stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 5 time sets

Platform:

GPL56

Series:

GSE18

5 Samples

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Dithiothrietol (DTT; 2.5 mM) added to culture and samples recovered at several time points up to 180 minutes. Included as part of a detailed characterization of global expression programs triggered by stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 8 time sets

Platform:

GPL53

Series:

GSE18

8 Samples

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Diamide (1.5 mM) added to culture, and samples recovered at several time points up to 90 minutes. Included as part of a detailed characterization of global expression programs triggered by stress.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 8 time sets

Platform:

GPL53

Series:

GSE18

8 Samples

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