GEO DataSets

(Submitter supplied) In response to limited nitrogen and abundant carbon sources, diploid Saccharomyces cerevisiae strains undergo a filamentous transition in cell growth as part of pseudohyphal differentiation. Use of the disaccharide maltose as the principal carbon source, in contrast to the preferred nutrient monosaccharide glucose, has been shown to induce a hyper-filamentous growth phenotype in a strain deficient for GPA2 which codes for a Galpha protein component that interacts with the glucose-sensing receptor Gpr1p to regulate filamentous growth. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

12 Samples

Download data: CEL

(Submitter supplied) Experiment design Type of experiment: Gene expression profiling analysis of S.cerevisiae W303-1a. Experimental factor: genetic variation (WT, gpr1 and gpa2 mutant strains). Number of hybridizations: 6. Single-colour labeling was performed for each sample. Sample preparation: Total RNA was prepared using FastRNA Pro Red Kit (BIO 101 Systems) according to the manufacturers' instructions. Labeling protocol 10 µg of total RNA was reverse transcribed using the SuperScript Choice system for cDNA synthesis (Life Technologies) according to the protocol recommended by Affymetrix. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS1485

Platform:

GPL90

6 Samples

Download data: CEL

Expression profiling of mutants lacking the Gpr1 receptor or the Gpa2 G protein alpha subunit. Results provide insight into the role of the Gpr1 receptor and the Gpa2 G protein alpha subunit in the maintenance and modulation of cell size in response to glucose.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 3 genotype/variation sets

Platform:

GPL90

Series:

GSE970

6 Samples

Download data: CEL

(Submitter supplied) Comparison of the transcriptomes of Saccharomyces cerevisiae wild type FY23 and a PDE2 deletion mutant DJ28. Keywords = PDE2 Keywords = Ras/cAMP pathway Keywords: other

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL422

10 Samples

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(Submitter supplied) There are about 800 genes in Saccharomyces cerevisiae whose transcription is cell-cycle regulated. Some of these form clusters of co-regulated genes. The 'CLB2' cluster contains 33 genes whose transcription peaks early in mitosis, including CLB1, CLB2, SWI5, ACE2, CDC5, CDC20 and other genes important for mitosis. Here we find that the genes in this cluster lose their cell cycle regulation in a mutant that lacks two forkhead transcription factors, Fkh1 and Fkh2. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2650 GPL51

26 Samples

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(Submitter supplied) Rsf1p is a putative transcription factor required for efficient growth using glycerol as sole carbon source but not for growth on the alternative respiratory carbon source ethanol. We use microarrays to determine the differences in the transcriptional program between the Δrsf1 mutant and the wild type during respiratory growth on glycerol as well as the transition to growth on glycerol as sole carbon source. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

22 Samples

Download data: CEL, CHP

(Submitter supplied) Transcriptional regulation of branched-chain amino acid metabolism in Saccharomyces cerevisiae involves two key regulator proteins, Leu3p and Gcn4p. Leu3p is a pathway-specific regulator, known to regulate six genes involved in branched-chain amino acid metabolism and one gene in nitrogen assimilation. Gcn4p is a global regulator, involved in the general response to amino acid and purine starvation. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS1103

Platform:

GPL90

12 Samples

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Analysis of leu3 mutant grown in either limited ethanol or limited ammonium media. Leu3p regulates a gene involved in nitrogen assimilation and six genes involved in branched chain amino acid metabolism. Results provide insight into the role of Leu3p in gene regulation.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 genotype/variation, 2 growth protocol sets

Platform:

GPL90

Series:

GSE2076

12 Samples

Download data

(Submitter supplied) Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae grown with six different nitrogen sources were subjected to transcriptome analysis. The use of chemostats enabled an analysis of nitrogen-source-dependent transcriptional regulation at a fixed specific growth rate. A selection of preferred (ammonium and asparagine) and non-preferred (leucine, phenylalanine, methionine and proline) nitrogen sources was investigated. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

15 Samples

Download data: CEL, EXP

(Submitter supplied) We used cDNA microarray technology to compare the genome-wide expression profiles of a wild type strain (BY4700) (E02, E04, E06) or the isogenic strain deleted of GLN3 and GAT1 genes (E01, E03, E05) grown in YNB medium with glutamine as nitrogen source (M.Gln) against the wild type strain grown in M.Gln after addition of rapamacyn (20 min) (E01, E02), or M.proline (M.Pro) (E03, E04) or after a two hours shift from M.Gln to M.Pro (E05, E06), all growth conditions known to modify the expression of genes involved in nitrogen utilization. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2602 GPL2600 GPL2603

20 Samples

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(Submitter supplied) The goal of this study is to analyse how the gene expression in Saccharomyces cerevisiae changes in dependency on the glucose concentration. Therefore, fed batch cultivations were carried out, during which the glucose concentration was maintained stable for several hours. Samples were taken at different times during the cultivations, the RNA was isolated and hybridised on whole genome yeast microarrays. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL537

7 Samples

Download data

(Submitter supplied) This study explores the connection between changes in gene expression and the genes that determine strain survival during suspension culture, using the model eukaryotic organism, Saccharomyces cerevisiae. The Saccharomyces cerevisiae homozygous diploid deletion pool, and the BY4743 parental strain were grown for 18 hours in a rotating wall vessel, a suspension culture device optimized to minimize the delivered shear. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL2544 GPL90

12 Samples

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(Submitter supplied) The SAGA complex of Saccharomyces cerevisiae contains greater than 20 components that acetylate and deubiquitylate nucleosomal histones. Its acetyltransferase, Gcn5 preferentially acetylates histones H3 and H2B and is regulated through interactions with Ada2 and Ngg1/Ada3. The N-terminal region of Ada2 contains a SANT domain that contacts Gcn5 near its catalytic site. Sequence alignments of Ada2 homologues indicate a conserved ~120 amino acid residue central region that interacts with Ngg1.To examine the function of this central region, we constructed ada2 alleles with mutations of clustered conserved residues. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array; Expression profiling by genome tiling array

Platforms:

GPL4131 GPL884

6 Samples

Download data: TXT

(Submitter supplied) We compared the genome-wide expression profiles of two yeast species (S. cerevisiae and S. paradoxus) using a two-species microarray that contain species-specific probes and can thus measure the expression levels of the two species simultaneosly. In Addition, we used the array to measure expression levels of the interspecific hybrid of these yeast species, while discriminating between the alleles that correspond to the two parental species. more...

Organism:

Saccharomyces cerevisiae; Saccharomyces paradoxus; Saccharomyces cerevisiae x Saccharomyces paradoxus

Type:

Expression profiling by array

Platform:

GPL8157

20 Samples

Download data: ZIP

(Submitter supplied) In contrast to batch cultivation, chemostat cultivation allows the identification of carbon source responses without interference by carbon-catabolite repression, accumulation of toxic products, and differences in specific growth rate. This study focuses on the yeast Saccharomyces cerevisiae, grown in aerobic, carbon-limited chemostat cultures. Genome-wide transcript levels and in vivo fluxes were compared for growth on two sugars, glucose and maltose, and for two C2-compounds, ethanol and acetate. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL90

12 Samples

Download data: CEL, EXP

(Submitter supplied) Wild-type diploid cells were shifted from yeast-form growth in SHAD liquid (plentiful glucose and ammonium) to filamentous-form growth on SLAD agar (low ammonium). Samples of filamentous-form cells were collected hourly for 10 hours. Filamentous-form and yeast-form exponential-phase targets were co-hybridized. Keywords: time-course

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS608

Platform:

GPL507

10 Samples

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Temporal analysis of wild type diploid cells shifted from yeast-form growth in SHAD liquid (plentiful glucose and ammonium) to filamentous-form growth on SLAD agar (low ammonium). Filamentous-form cells collected hourly for 10 hours.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, log ratio, 10 time sets

Platform:

GPL507

Series:

GSE679

10 Samples

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(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Saccharomyces cerevisiae S288C

Type:

Expression profiling by array

Platform:

GPL21316

8 Samples

Download data: GPR

(Submitter supplied) The study on the effect of nitrogen replenishment on the gene expression following a rapamycin treatment showed that the down-regulation of the glycolytic transcripts was generally suppressed after this treatment. This indicated that the TOR signaling pathway likely controls the expression of the glycolytic gene under these conditions.

Organism:

Saccharomyces cerevisiae S288C

Type:

Expression profiling by array

Platform:

GPL21316

4 Samples

Download data: GPR

(Submitter supplied) The study on the effect of nitrogen replenishment on the gene expression showed that 390 genes were significantly upregulated (with a logFC ranging from 4.5 to 0.8), among which those related to RNA binding, ribosome binding, structural constituent of ribosome, transferase activity and transcription. 833 genes were significantly down-regulated (with a logFC ranging from -0.8 to -5.2) including those related to stress response, amino-acid catabolism process, protein catabolic process (ubiquitin-dependent and vacuolar protein degradation). more...

Organism:

Saccharomyces cerevisiae S288C

Type:

Expression profiling by array

Platform:

GPL21316

4 Samples

Download data: GPR