GEO DataSets

(Submitter supplied) Regulation of gene expression is essential for normal development and cellular growth. Transcriptional events are tightly controlled both spatially and temporally by specific DNA-protein interactions. In this study we finely map the genome-wide targets of the CREB protein across all known and predicted human promoters, and characterize the functional consequences of a subset of these binding events using high-throughput reporter assays. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL9325

9 Samples

Download data: TXT

(Submitter supplied) Gene expression analysis to search for genes which are up- and down-regulated after CacyBP/SIP overexpression in HCT116 cells

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

8 Samples

Download data: TXT

(Submitter supplied) PC12 cells (passage 22) were cultured at 5% CO2 in a 37 C incubator. The growth medium was DMEM (high glucose, Invitrogen) supplemented with 25mM HEPES, 10% FCS, 5% FBS and 1x Pen/Strep (Invitrogen). 1x106 cells were starved overnight in DMEM+25 mM HEPES and treated with 10 uM 1,9 dideoxy forskolin or forskolin for 60 min. The final DMSO concentration was 0.05%. Total RNA was purified using Trizol (Invitrogen) according to the manufacturers protocol. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS1038 GDS1039

Platforms:

GPL342 GPL341

8 Samples

Download data

Expression profiling of PC12 pheochromocytoma cells treated with 10 uM forskolin for 1 hour. Forskolin activates adenylate cyclase. Effect of 1,9 dideoxy forskolin, an inactive analog of forskolin, also examined. Results identify genes whose expression responds to changes in cAMP levels.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL342

Series:

GSE2071

4 Samples

Download data

Expression profiling of PC12 pheochromocytoma cells treated with 10 uM forskolin for 1 hour. Forskolin activates adenylate cyclase. Effect of 1,9 dideoxy forskolin, an inactive analog of forskolin, also examined. Results identify genes whose expression responds to changes in cAMP levels.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL341

Series:

GSE2071

4 Samples

Download data

(Submitter supplied) We generated whole-genome gene expression profiles of F11 dorsal root ganglion (DRG)-like neuroblastoma cells stimulated with the adenylate cyclase activator forskolin for 0, 2, 4, 24 and 48 hours.

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL4135

12 Samples

Download data: TXT

(Submitter supplied) E2F2 is essential for the maintenance of T lymphocyte quiescence. To identify the full set of E2F2 target genes, and to gain further understanding of the role of E2F2 in transcriptional regulation, we have performed ChIP-chip analyses across the genome of lymph node-derived T lymphocytes. Here we show that during quiescence, E2F2 binds the promoters of a large number of genes involved in DNA metabolism and cell cycle regulation, concomitant with their transcriptional silencing.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL18145

6 Samples

Download data: GFF, PAIR, TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by array

Platforms:

GPL11002 GPL10333

16 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Metabolic homeostasis in mammals critically depends on the regulation of fasting-induced genes by CREB in the liver. Previous genome-wide analysis has shown that only a small percentage of CREB target genes are induced in response to fasting-associated signaling pathways. The precise molecular mechanisms by which CREB specifically targets these genes in response to alternating hormonal cues remain to be elucidated. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10333

4 Samples

Download data: TXT

(Submitter supplied) Metabolic homeostasis in mammals critically depends on the regulation of fasting-induced genes by CREB in the liver. Previous genome-wide analysis has shown that only a small percentage of CREB target genes are induced in response to fasting-associated signaling pathways. The precise molecular mechanisms by which CREB specifically targets these genes in response to alternating hormonal cues remain to be elucidated. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL11002

12 Samples

Download data: BED, BEDGRAPH

(Submitter supplied) RNA Polymerase-II active regions were mapped comparing mouse neural retina tissue at age P25 and P2 to find novel gene activation predictions during maturation of photoreceptors. Over 800 predictions of increased activation were novel compared to previous mRNA expression array studies.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL5811

3 Samples

Download data: BED, CEL

(Submitter supplied) The CREB family of transcription factors stimulates cellular gene expression following phosphorylation at a conserved serine (Ser133 in CREB1) in response to cAMP and other extracellular signals. In order to characterize CREB target genes in various tissues, we give a cAMP agonist, forskolin (FSK), to cell lines or primary cultures and monitor the gene expression. To eliminate CREB-independent effects of FSK on cellular gene expression, we employed a dominant negative form of CREB called A-CREB, which dimerizes selectively with and blocks the DNA binding activity of CREB but not other bZIP family members. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by array

4 related Platforms

27 Samples

Download data: CEL, EXP

(Submitter supplied) Vasopressin, a peptide hormone, controls renal water excretion, largely through regulation of water channel aquaporin-2 (AQP2) in the renal collecting duct. There are two regulatory mechanisms of AQP2: 1) short-term regulation by membrane trafficking of AQP2; and 2) long-term regulation involving vasopressin-induced changes of protein abundance of AQP2 through regulation of gene transcription and protein half-life. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL21493

4 Samples

Download data: BED, BW

(Submitter supplied) Vasopressin, a peptide hormone, controls renal water excretion, largely through regulation of water channel aquaporin-2 (AQP2) in the renal collecting duct. There are two regulatory mechanisms of AQP2: 1) short-term regulation by membrane trafficking of AQP2; and 2) long-term regulation involving vasopressin-induced changes of protein abundance of AQP2 through regulation of gene transcription and protein half-life. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

22 Samples

Download data: BED, BEDGRAPH

(Submitter supplied) Genome-wide location analysis of TAF1 and RNA polymerase II binding in ENCODE regions of IMR90, HCT116, HeLa and THP1 cell lines. There are a total of 8 experiments, each with three replicates. Details of chromatin immunoprecipitation and data analysis can be found at Li Z, van Calcar S, Qu C, Cavenee WK, Zhang MQ, Ren B (2003) A global transcriptional regulatory role for c-Myc in Burkitt's lymphoma cells. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by genome tiling array

Platform:

GPL1454

24 Samples

Download data: TIFF, TXT