GEO DataSets

(Submitter supplied) The transcriptional profile of the porcine lung pathogen, Actinobacillus pleuropneumoniae, was monitored during the acute phase of infection in its natural host. Bacterial expression profiles of A. pleuropneumoniae isolated from lung lesions of 25 infected pigs were compared in samples taken 6, 12, 24 and 48 hours post infection.

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL11003

75 Samples

Download data: TXT, XYS

(Submitter supplied) This study aimed at: (1) Establishing the pleuropneumoniae pathological model of artificial infection; (2) Using the Agilent swine genome-wide microarray containing 43,603 probes to detect the changes in gene expression of infected pigs’ lungs and hilar nodes.

Organism:

Sus scrofa

Type:

Expression profiling by array

Platform:

GPL10162

12 Samples

Download data: TXT

(Submitter supplied) The objective of this study was to identify porcine genes which expression was affected by experimental infection with A. pleuropneumoniae within 24 hours after experimental challenge. Microarray experiments were conducted to reveal genes being significantly differentially expressed in infected versus non-infected lung tissue and in liver and lung lymph node tissue from three challenged versus two non-challenged animals. more...

Organism:

Sus scrofa

Type:

Expression profiling by array

Platforms:

GPL3594 GPL3585

16 Samples

Download data: CSV

(Submitter supplied) To reveal the transcriptional profiles of Actinobacillus pleuropneumoniae under biofilm and planktonic growth, we established a biofilm-forming culture method and constructed a mutant strain Δpga with defect in biofilm formation. Wild-type and Δpga mutant strains of Actinobacillus pleuropneumoniae strain 4074 were cultured in bottles with shaking for planktonic (WT_PK) and in microplates in static status for biofilm (WT_BF, Δpga), respectively. more...

Organism:

Actinobacillus pleuropneumoniae serovar 1 str. 4074

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32988

9 Samples

Download data: TXT

(Submitter supplied) Host-pathogen interactions are of great importance in understanding the pathogenesis of infectious microorganisms. We developed in vitro models to study porcine respiratory tract pathogens using an immortalized epithelial cell line, the St. Jude Porcine Lung (SJPL) cell line. In conditions where cytotoxicity was absent or low, the transcriptomic profile of A. pleuropneumoniae after contact with the porcine lung epithelial cells was determined using DNA microarrays. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL3648

8 Samples

Download data: MEV

(Submitter supplied) Nitrate metabolism is an adaptation mechanism used by many bacteria for survival under anaerobic environments. As a by-product of inflammation, nitrate is used by the intestinal bacterial pathogens to enable gut infection. However, the responses of bacterial respiratory pathogens to nitrate are less well understood. Actinobacillus pleuropneumoniae is an important bacterial respiratory pathogen of swine. more...

Organism:

Actinobacillus pleuropneumoniae serovar 1 str. 4074

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32420

18 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 5b str. L20

Type:

Expression profiling by array

Platform:

GPL6658

16 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae dripbiofilm biofilms was compared to the transcriptome of effluent cells

Organism:

Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 5b str. L20

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae 4h static biofilms was compared to the transcriptome of 6h static biofilm

Organism:

Actinobacillus pleuropneumoniae serovar 5b str. L20; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

Organism:

Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 5b str. L20

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) The transcriptome of Actinobacillus pleuropneumoniae biofilms was compared to the transcriptome of planktonic bacteria

Organism:

Actinobacillus pleuropneumoniae serovar 5b str. L20; Actinobacillus pleuropneumoniae

Type:

Expression profiling by array

Platform:

GPL6658

4 Samples

Download data: MEV

(Submitter supplied) Acute pleuropneumonia in swine, caused by Actinobacillus pleuropneumoniae (APP), is characterized by a high and sustained fever. Fever creates an adverse environment for many bacteria leading to reduced bacterial proliferation; however, most pathogenic bacteria can tolerate higher temperatures. CpxAR is a two-component regulation system, ubiquitous among Gram-negative bacteria, which senses and responds to envelope alterations that are mostly associated with protein misfolding in the periplasm. more...

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30447

6 Samples

Download data: TXT

(Submitter supplied) Bacteria can use host hormones as environment cue to modulate their pathogenic processes, which was discovered to play essential role in disease development. Actinobacillus pleuropneumoniae is one of the most important porcine respiratory pathogens causing great economic losses in the pig industry worldwide. Stress factors were found to contribute to the outcome of A. pleuropneumoniae infection. To test whether A. more...

Organism:

Actinobacillus pleuropneumoniae; Actinobacillus pleuropneumoniae serovar 1 str. 4074

Type:

Expression profiling by array

Platform:

GPL9691

6 Samples

Download data: TXT

(Submitter supplied) Actinobacillus pleuropneumoniae is the cause of porcine pleuropneumonia, a severe respiratory tract infection that is responsible for major economic losses to the swine industry. Many host-adapted bacterial pathogens encode systems known as phasevarions (phase-variable regulons). Phasevarions result from variable expression of cytoplasmic DNA methyltransferases. Variable expression results in genome-wide methylation differences within a bacterial population, leading to altered expression of multiple genes via epigenetic mechanisms. more...

Organism:

Escherichia coli; Actinobacillus pleuropneumoniae

Type:

Other

Platforms:

GPL33074 GPL33073

16 Samples

Download data: RDS

(Submitter supplied) Understanding constraints which shape antibiotic resistance is key for predicting and controlling drug resistance. Here, we performed high-throughput laboratory evolution of Actinobacillus pleuropneumoniae and its ciprofloxacin resistance-inducing derivatives.This study aims to explore the mechanism of acquired ciprofloxacin resistance in Actinobacillus pleuropneumoniae.

Organism:

Actinobacillus pleuropneumoniae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL30447

6 Samples

Download data: FASTA, GFF3, TXT

(Submitter supplied) Peripheral blood mononuclear cells (PBMCs) were isolated from the blood of pigs at different time points (0, 24 and 120 h) post infection and their transcriptome determined by Illumina HiSeq 4000 high-throughput sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the DEGs revealed the close relationship between the peripheral blood and inflammatory response from the perspective of molecular function, cell composition, biological processes and signalling pathways.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22475

3 Samples

Download data: TXT