GEO DataSets

(Submitter supplied) The study aimed to identify miRNAs expression profiles associated with growth and regression of dominant-size follicles in bovine. Follicles and corpora lutea (CL, from days 1 to 4 of the estrous cycle) were collected from abattoir ovaries and their status (healthy/atretic) was assessed by measuring steroid levels and aromatase expression. Total RNA was isolated from whole follicles at different developmental stages and from CL. more...

Organism:

Rattus norvegicus; JC polyomavirus; Murid gammaherpesvirus 4; Betapolyomavirus hominis; Human alphaherpesvirus 1; Human alphaherpesvirus 2; human gammaherpesvirus 4; Mus musculus cytomegalovirus 2; Betapolyomavirus macacae; Homo sapiens; Bos taurus; Human betaherpesvirus 5; Murid betaherpesvirus 1; Human immunodeficiency virus 1; Merkel cell polyomavirus; Mus musculus; Human gammaherpesvirus 8

Type:

Non-coding RNA profiling by array

Platform:

GPL11434

18 Samples

Download data: TXT

(Submitter supplied) Development of ovarian follicles is controlled at the molecular level by several gene products whose precise expression leads to regression or ovulation of follicles. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through sequence-specific base pairing with target messenger RNAs (mRNAs) causing translation repression or mRNA degradation. The aim of this study was to identify miRNAs expressed in ovarian follicles, localize their expression within the theca and/or granulosa layers and their putative target genes/pathways that are involved in bovine ovarian follicle development. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by array

Platform:

GPL18422

24 Samples

Download data: TXT

(Submitter supplied) This study was conducted to investigate the miRNA enrichment and degradation in the granulosa cells of dominant and subordinate follicle during the early luteal phase of the bovine estrous cycle using high throughput miRNA sequencing technology.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

12 Samples

Download data: CSV

(Submitter supplied) The study aimed to uncover differential expression pattern of regulatory microRNAs in bovine granulosa cells derived from preovulatory dominant and subordinate follicles.

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

6 Samples

Download data: CSV

(Submitter supplied) In this study, we determined the miRNA expression profile of bovine alveolar macrophages, using next-generation sequencing strategy. On an Illumina HiSeq 2000 machine, we sequenced 8 miRNA libraries, prepared from small RNA fractions of alveolar macrophages isolated from 8 different healthy animals (Bos taurus). From the data, the potential novel miRNAs were predicted, and the expression levels of the known miRNAs were determined. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL15749

8 Samples

Download data: TXT

(Submitter supplied) Granulosa cells originating from two different phases of antral follicle growth (plateau and atretic) were compared in follicles with a diameter of 9mm or more. The plateau follicle is the reference.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

8 Samples

Download data: TXT

(Submitter supplied) Granulosa cells originating from two different phases of antral follicle growth (growing and atresia) were compared in follicles with a diameter of 9mm or more. Growing follicle is the reference.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

8 Samples

Download data: TXT

(Submitter supplied) Granulosa cells originating from two different phases of antral follicle growth (growing and plateau) were compared in follicles with a diameter of 9mm or more. Growing follicle is the reference.

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

8 Samples

Download data: TXT

(Submitter supplied) We tried to classify largest (F1) and second largest follicles (F2) by global gene expression profiles and to identify differentially expressed genes between the follicular groups using combination of microarray and quantitative real-time PCR (QPCR) analysis. F1 (10.7 ± 0.7 mm) and F2 (7.8 ± 0.2 mm) were collected for microarray and QPCR analysis. Global gene expression profiles of each follicle were analyzed by hierarchical cluster analysis and expression profiles of representative 16 genes were confirmed by QPCR analysis. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL9136

5 Samples

Download data: TXT

(Submitter supplied) Goal:Circular RNAs (circRNAs) are thought to play important roles in multiple biological processes including apoptosis. In the ovary granulosa cell apoptosis plays an essential role in the follicular phase of the estrous cycle as it determines if the selected follicles will degenerate or escape this fate and ovulate. Whether circRNAs play a role in granulosa cell apoptosis is at present unknown. By investigating the potential role of circRNAs in the granulosa cell apoptotic cascade we hope to gain novel information regarding the regulation of antral follicular apoptosis using the porcine ovary as a model. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

6 Samples

Download data: XLSX

(Submitter supplied) Granulosa cells mature and die as ovarian follicles enlarge and die (undergo atresia) under the influence of hormones and intrafollicular factors. Later in follicular development, a fluid-filled antrum is formed, a process which is accompanied by a high rate of atresia. These small antral follicles (5 mm or less in diameter in the cow) contain granulosa of 2 different phenotypes, rounded or columnar, whereas follicles larger than 5 mm have the rounded phenotype only. more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL2112

19 Samples

Download data: CEL

(Submitter supplied) MicroRNAs (miRNAs) are small noncoding RNAs that participate in regulation of gene expression. Their role during mammary gland development is still largely unknown. In the present study, we performed a microarray analysis to identify miRNAs associated with high mammogenic potential of bovine mammary gland. We identified 54 miRNAs differing significantly between mammary tissue of dairy (Holstein-Friesian, HF) and beef (Limousine, LM) post-pubertal heifers. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by array

Platform:

GPL19170

8 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are small noncoding RNAs that regulate gene expression primarily at the post-transcriptional levels and thereby play important roles in regulating many physiological and developmental processes. Oocyte maturation in fish is induced by hormones produced from the hypothalamus, pituitary, and ovary. Gonadotropin-releasing hormone (GnRH) stimulates the secretion of luteinizing hormone (LH), which in turn, induces the secretion of maturation-inducing hormone (MIH) from the ovary. more...

Organism:

Danio rerio

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14875

6 Samples

Download data: TXT

(Submitter supplied) Cell-free RNAs have been used as diagnostic disease markers describing the state of tissue environment. The origin and potential functions of such RNAs in human pre-ovulatory follicle, the environment of oocyte maturation, are unclear. We aimed to describe the small RNA profiles of three follicular compartments of the same follicle: mural granulosa cells (MGC), cell-free follicular fluid (FF) and extracellular vesicles (EV) of the FF. more...

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18573

45 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) are short non-coding RNAs that post-transcriptionally regulate expression of mRNAs in many biological pathways. Here we report comprehensive miRNAs profiles by next-gen deep sequencing in Angus cattle divergently selected for residual feed intake (RFI) and identify miRNAs related to feed efficiency in beef cattle Results: Two microRNA libraries were constructed from pooled RNA extracted from livers of low and high RFI cattle, and sequenced by Illumina genome analyser. more...

Organism:

Bos taurus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL11153

2 Samples

Download data: TXT

(Submitter supplied) Purpose: We aimed to investigate important miRNA events and to define specific miRNA expression signature underlying the follicle activation of zebrafish. Methods: By using small and regular RNA-sequencing, we performed transcriptomic analyses of PG (primary growth stage I; inactive) and PV (pre-vitellogenic stage II; activated) follicles to decipher important miRNA and gene events underlying follicle activation of zebrafish. more...

Organism:

Danio rerio

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL14875

4 Samples

Download data: TXT

(Submitter supplied) MicroRNAs (miRNAs) have known roles in the post-transcriptional regulation of various biological processes including ovarian follicle development. We have previously identified from human pre-ovulatory granulosa cells miRNAs that are expressed from the intronic regions of two key genes in normal follicular development: FSH receptor (FSHR) and CYP19A1, the latter encoding the aromatase enzyme. In the present study, we aim to identify the targets regulated by those two miRNAs: hsa-miR-548ba and hsa-miR-7973, respectively. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL16686

12 Samples

Download data: CEL

(Submitter supplied) The study aimed to identify miRNAs expression profiles associated with growth and regression of dominant-size follicles in bovine. Follicles were collected from abattoir ovaries and their status (healthy/atretic) was assessed by measuring steroid levels and aromatase expression. Corpora lutea (CL) were collected from ovarian pairs correponding to days 1 to 4 of the oestrus cycle. Total RNA was isolated from whole follicles at different developmental stages and from CL. more...

Organism:

Rattus norvegicus; Human alphaherpesvirus 2; Murid gammaherpesvirus 4; Betapolyomavirus hominis; Mus musculus; Murid betaherpesvirus 1; JC polyomavirus; Human immunodeficiency virus 1; Human gammaherpesvirus 8; Homo sapiens; Bos taurus; Merkel cell polyomavirus; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; Mus musculus cytomegalovirus 2; Betapolyomavirus macacae

Type:

Non-coding RNA profiling by array

Platform:

GPL11434

24 Samples

Download data: TXT

(Submitter supplied) Cows in Negative Energy Balance (NEB) may preferentially divert nutrients away from reproduction, thereby experiencing a period of anovulatory anestrus, delayed ovulation of large follicles and a condition of impaired fertility. To better understand the changes occurring in these large follicles as a function of time post-partum granulosa cells of preovulatory follicles have been collected at different times: 30, 60, 90 and 120 days after calving . more...

Organism:

Bos taurus

Type:

Expression profiling by array

Platform:

GPL13226

9 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bos taurus

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platforms:

GPL19172 GPL23295

4 Samples

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