GEO DataSets

(Submitter supplied) We used RNA-seq to investigate gene expression variation in Malpighian tubules, which have a function analogous to that of human kidneys. In order to characterize population differentiation, we sequenced the Malpighian tubule transcriptomes of flies derived from two populations, one from sub-Saharan Africa (Zimbabwe) and one from Europe (the Netherlands). Males and females were examined separately. Overall, we found a high amount of differential expression between sexes (2,308 genes) and populations (2,474 genes). Although most of the differentially expressed genes were consistent between sexes and populations, there were 615 genes showed sex-biased expression in only one population and 557 genes showed population-biased expression in only one sex.

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

8 Samples

Download data: TXT

(Submitter supplied) Background: Changes in gene regulation are thought to be crucial for the adaptation of organisms to their environment. Transcriptome analyses can be used to identify candidate genes for ecological adaptation, but can be complicated by variation in gene expression between tissues, sexes, or individuals. Here we use high-throughput RNA sequencing of a single Drosophila melanogaster tissue to detect brain-specific differences in gene expression between the sexes and between two populations, one from the ancestral species range in sub-Saharan Africa and one from the recently colonized species range in Europe. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

8 Samples

Download data: TXT

(Submitter supplied) We used RNA-seq to investigate natural variation in gene expression in the Malpighian tubules of three inbred Drosophila melanogaster strains and their F1 hybrids. One of the strains was from a population in the species’ ancestral range (Zambia), while the other two were from a more recently derived population (Sweden).

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17275

12 Samples

Download data: TXT

(Submitter supplied) Genes with sex-biased expression often show rapid molecular evolution between species. Previous population genetic and comparative genomic studies of Drosophila melanogaster and D. simulans revealed that male-biased genes have especially high rates of adaptive evolution. To test if this is also the case for other lineages within the melanogaster group, we investigated gene expression in D. ananassae, a species that occurs in structured populations in tropical and subtropical regions. more...

Organism:

Drosophila ananassae

Type:

Expression profiling by array

Platform:

GPL9699

12 Samples

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(Submitter supplied) Background: Differences in levels of gene expression among individuals are an important source of phenotypic variation within populations. Recent microarray studies have revealed that expression variation is abundant in many species, including Drosophila melanogaster. However, previous expression surveys in this species generally focused on a small number of laboratory strains established from derived populations. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL3830

80 Samples

Download data: GPR

(Submitter supplied) To investigate the effect of sex on within- and between-population variation in gene expression, we performed a microarray analysis of adult females from 16 strains of Drosophila melanogaster, including eight strains from the putative ancestral range in sub-Saharan Africa and eight strains from a European population. The results were compared to those of a previous study of adult male gene expression variation among the same strains (GSE8843).

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL3603

56 Samples

Download data: GPR

(Submitter supplied) A number of genes associated with sexual traits and reproduction evolve at the sequence level faster than the majority of genes coding for non-sex-related traits. Whole genome analyses allow this observation to be extended beyond the limited set of genes that have been studied thus far. We use cDNA microarrays to demonstrate that this pattern holds in Drosophila for the phenotype of gene expression as well, but in one sex only. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Datasets:

GDS438 GDS439 GDS440 GDS441

Platform:

GPL356

23 Samples

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(Submitter supplied) 5928 cDNAs from the Drosophila Gene Collection version 1.0 were amplified by PCR with universal primers and PCR products were confirmed by gel electrophoresis. Added to these was a set of 177 separately amplified controls, each of which was replicated from one to 16 times on the array. Clones for which the PCR product was absent, faint, the incorrect size, or showed multiple bands failed PCR quality control. more...

Organism:

Drosophila melanogaster

4 DataSets

3 Series

56 Samples

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Analysis of intraspecific expression differentiation shows that gene expression in males evolves more rapidly than in females. Canton S, Oregon R, Hikone R, a St. Louis-derived isofemale strain and four Zimbabwe-derived lines examined.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, log ratio, 5 strain sets

Platform:

GPL356

Series:

GSE539

5 Samples

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Analysis of intraspecific expression differentiation shows that gene expression in males evolves more rapidly than in females. Canton S, Oregon R, Hikone R, a St. Louis-derived isofemale strain and four Zimbabwe-derived lines examined.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, log ratio, 6 strain sets

Platform:

GPL356

Series:

GSE539

6 Samples

Download data

Analysis of intraspecific expression differentiation shows that gene expression in males evolves more rapidly than in females. Canton S, Oregon R, Hikone R, a St. Louis-derived isofemale strain and four Zimbabwe-derived lines examined.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, log ratio, 7 strain sets

Platform:

GPL356

Series:

GSE539

7 Samples

Download data

Analysis of intraspecific expression differentiation shows that gene expression in males evolves more rapidly than in females. Canton S, Oregon R, Hikone R, a St. Louis-derived isofemale strain and four Zimbabwe-derived lines examined.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, log ratio, 6 strain sets

Platform:

GPL356

Series:

GSE539

6 Samples

Download data

(Submitter supplied) To identify the transcriptome divergence and sex roles underlying it in recently diverged Drosophia pseudoobscura group, we compare the global gene expression of 7-day-old whole body adults of pure species.

Organism:

Drosophila pseudoobscura; Drosophila pseudoobscura bogotana; Drosophila persimilis

Type:

Expression profiling by array

Platform:

GPL8884

18 Samples

Download data: GPR

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL6799

88 Samples

Download data: GPR

(Submitter supplied) Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. Genes were identified that were expressed during metamorphosis in both somatic and germline tissues of males and females. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL6799

60 Samples

Download data: GPR

(Submitter supplied) Drosophila melanogaster undergoes a complete metamorphosis, during which time the larval male and female forms transition into sexually dimorphic, reproductive adult forms. To understand this complex morphogenetic process at a molecular-genetic level, we performed whole genome microarray analyses. In somatic tissues at 48 hour After Puparium Formation (APF), 173 sex-biased transcripts that likely function downstream of the doublesex (dsx) branch of the sex determination hierarchy were identified. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL6799

28 Samples

Download data: GPR

(Submitter supplied) The comparison of transcriptome profiles among populations is a powerful tool for investigating the role of gene expression change in adaptation to new environments. In this study, we use massively parallel sequencing of 39 cDNAs obtained from large samples of adult males, to compare a population of Drosophila simulans from a natural reserve within its ancestral range (eastern Africa) with a derived population collected in the strongly anthropized Rhone valley (France). more...

Organism:

Drosophila simulans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17484

4 Samples

Download data: TXT

(Submitter supplied) Transcriptome analysis may provide means to investigate the underlying genetic causes of shared and divergent phenotypes in different populations and help to identify potential targets of adaptive evolution. Applying RNA sequencing to whole male Drosophila melanogaster from the ancestral tropical African environment and a very recently colonized cold-temperate European environment at both standard laboratory conditions and following a cold shock, we seek to uncover the transcriptional basis of cold adaptation. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13304

64 Samples

Download data: TXT

(Submitter supplied) Wild-type laboratory strains of model organisms are typically kept in isolation for many years, with the action of genetic drift and selection on mutational variation causing lineages to diverge with time. Natural populations from which such strains are established, show that gender-specific interactions in particular drive many aspects of sequence level and transcriptional level variation. Here, our goal was to identify genes that display transcriptional variation between laboratory strains of Drosophila melanogaster, and to explore evidence of gender-biased interactions underlying that variability. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Dataset:

GDS3639

Platform:

GPL72

29 Samples

Download data: CEL

Analysis of male and female flies of 5 wild-type laboratory strains. Results provide insight into the extent to which transcriptional variation is inherent in commonly used laboratory strains and the relationship of variation in gene expression to sexual dimorphism.

Organism:

Drosophila melanogaster

Type:

Expression profiling by array, transformed count, 2 gender, 5 strain sets

Platform:

GPL72

Series:

GSE9149

29 Samples

Download data: CEL