GEO DataSets

(Submitter supplied) Human bone marrow mesenchymal stem cells (hBM-MSCs) hold promise for treating diseases for which currently no therapeutic options exist. High quantities of MSCs are needed, requiring extensive cell expansion in long-term culture. However, the fundamental biological properties of MSCs can be altered by culture conditions. In this study, hBM-MSCs were isolated from residual human bone marrow (hBM) material and expanded to clinically relevant numbers at passage 3-4. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL20203

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platforms:

GPL20203 GPL20206 GPL20204

36 Samples

Download data

(Submitter supplied) Human bone marrow mesenchymal stem cells (hBM-MSCs) hold promise for treating diseases for which currently no therapeutic options exist. High quantities of MSCs are needed, requiring extensive cell expansion in long-term culture. However, the fundamental biological properties of MSCs can be altered by culture conditions. In this study, hBM-MSCs were isolated from residual human bone marrow (hBM) material and expanded to clinically relevant numbers at passage 3-4. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL20206

12 Samples

Download data: TXT

(Submitter supplied) Human bone marrow mesenchymal stem cells (hBM-MSCs) hold promise for treating diseases for which currently no therapeutic options exist. High quantities of MSCs are needed, requiring extensive cell expansion in long-term culture. However, the fundamental biological properties of MSCs can be altered by culture conditions. In this study, hBM-MSCs were isolated from residual human bone marrow (hBM) material and expanded to clinically relevant numbers at passage 3-4. more...

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL20204

12 Samples

Download data: TXT

(Submitter supplied) Global gene expressions of human bone-derived 11Lin-CD45-CD271+SSEA-4+ mesenchymal stem/stromal cells from young and elderly patients were analyzed. Results provide an insight into the molecular mechanisms of aging and cellular senescence.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL20844

6 Samples

Download data: TXT

(Submitter supplied) To compare the gene expression profile of hBM-MSCs at early (P3), late-passage (P7), and UBC gene knockdown (KD) from three different donors, we performed one-color microarray-based gene expression analysis.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL17077

10 Samples

Download data: TXT

(Submitter supplied) Gene Markers of Cellular Aging in Human Multipotent Stromal Cells in Culture Identifying gene markers of cellular aging as determined by cellular passaging of human multipotent stromal cells (MSCs) derived from bone marrow

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL18503

63 Samples

Download data: TXT

(Submitter supplied) Heterogeneous populations of human bone marrow-derived stromal cells (BMSC) are among the most frequently tested cellular therapeutics for treating degenerative and immune disorders, which occur predominantly in the aging population. Currently, it is unclear whether advanced donor age and commonly associated comorbidities affect the properties of ex vivo-expanded BMSCs. Thus, we stratified cells from adult and elderly donors from our biobank (n = 10 and n = 13, mean age 38 and 72 years, respectively) and compared their phenotypic and functional performance, using multiple assays typically employed as minimal criteria for defining multipotent mesenchymal stromal cells (MSCs).We found that BMSCs from both cohorts meet the standard criteria for MSC, exhibiting similar morphology, growth kinetics, gene expression profiles, and pro-angiogenic and immunosuppressive potential and the capacity to differentiate toward adipogenic, chondrogenic, and osteogenic lineages.We found no substantial differences between cells from the adult and elderly cohorts. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL18460 GPL20301

40 Samples

Download data: TXT

(Submitter supplied) In this study, we investigated differential genetic expression patterns among TMSCs with different cultivation times and passage numbers. Transcriptomic approaches were used to further identify novel candidate biomarkers of culture-aged, senescent TMSCs. These analyses have led us to propose that genes associated with ECM are differentially altered with aging of TMSCs, and some of these molecules could be used as potential indicators for identifying stem cells with senescence.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL23126

4 Samples

Download data: CEL

(Submitter supplied) We previously showed that the combination of markers LNGFR+ and THY-1+ can be used selectively to isolate human mesenchymal stem cells (hMSCs) in bone marrow and several tissues. However, the molecular mechanisms regulating stemness of hMSCs remain to be elucidated. In this study, we found that Frizzled 5 (FZD5) and Receptor tyrosine kinase-like orphan receptor 2 (ROR2) were highly enriched in LNGFR+THY-1+ derived clones that retain a high potential for proliferation (rapidly expanding clones; RECs). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6480

8 Samples

Download data: TXT

(Submitter supplied) Mesenchymal stromal cells (MSCs) are of high relevance for the regeneration of mesenchymal tissues such as bone and cartilage. The promising role of MSCs in cell-based therapies and tissue engineering appears to be limited due to a decline of their regenerative potential with increasing donor age, their limited availability in human tissues and the need of in vitro expansion prior to treatment. We therefore aimed to determine to which degree in vitro aging and chronological aging may be similar processes or if in vitro culture-related changes at the cellular and molecular level are at least altered as a function of donor age. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL8031

18 Samples

Download data: TXT

(Submitter supplied) Bone marrow stromal cells (BMSCs) can be expanded by serial passage, but expansion is limited by cell senescence.  The nature of changes associated with BMSC serial passages was assessed. Transcriptome analysis of 10 early and 15 late passage samples from 5 subjects revealed 2193 differentially expressed genes; those highly expressed in early passage cells were overrepresented in skeletal system development, embryonic morphogenesis, tube morphogenesis, etc, while those highly expressed in the late passage BMSCs were overrepresented in nucleosome assembly; chromatin assembly, DNA packaging, etc. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

57 Samples

Download data: TXT

(Submitter supplied) Cells in culture undergo replicative senescence and unequivocally stop proliferation after a limited number of cell divisions. In this study, we have expanded mesenchymal stem cells (MSC) from human adipose tissue and analyzed genetic and epigenetic sequels. The subpopulation of highly proliferative cells and the in vitro differentiation potential decayed already within early passages. Relevant chromosomal aberrations were not detected by karyotyping and SNP-microarrays.

Organism:

Homo sapiens

Type:

Genome variation profiling by SNP array

Platform:

GPL6801

10 Samples

Download data: CEL, CNCHP

(Submitter supplied) Cells in culture undergo replicative senescence. In this study, we analyzed functional, genetic and epigenetic sequels of long‐term culture in human mesenchymal stem cells (MSC). Already within early passages the fibroblastoid colony‐ forming unit (CFU‐f) frequency and the differentiation potential of MSC declined significantly. Relevant chromosomal aberrations were not detected by karyotyping and SNP‐microarrays. more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL8490

8 Samples

Download data: TXT

(Submitter supplied) In this study, we have addressed how cellular senescence influences the immunomodulatory potential of human mesenchymal stem cells (hMSCs). We induced cell senescence in a panel of bone marrow-derived hMSC samples by means of gamma-irradiation, and performed both gene expression and miRNA microarray analyses on the untreated and senescent samples. We also compared the gene expression profile of untreated and senescent hMSCs with those obtained from several hMSCs samples used in an ongoing allogeneic clinical study of Graft Versus Host Disease (GVHD), of which their therapeutic efficacy is known. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL13497 GPL10850

32 Samples

Download data: TXT

(Submitter supplied) The properties of MSCs can be affected by long term culture, therefore casting doubt over the ability of late passage MSCs to accurately recapitulate their biology. Additionally, assumption within the scientific community is often made that early passage MSCs are still representative of the primary MSC population, however, little research has been done to support this. We compared the transcriptomic profiles of murine MSCs freshly isolated from the long bones to MSCs that had been expanded in culture for 10 days. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

6 Samples

Download data: XLSX

(Submitter supplied) To determine miRNA expression changes during in vitro senescence of mesenchymal stem cells (MSC) we have analyzed differential expression of the corresponding early passage (P2) and senescent passage (PX). Keywords: miRNA, senescence, mesenchymal stromal cells, mesenchymal stem cells, MSC

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL6127

14 Samples

Download data: GPR

(Submitter supplied) To determine gene expression changes during in vitro senescence of MSC we have analyzed differential expression of the corresponding early passage (P2) and senescent passage (PX). There were global changes in the gene expression profile that were reproducible in three independent donor samples. Keywords: Replicative senescence, time course

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

13 Samples

Download data: CEL, CHP

(Submitter supplied) It is now well established that bone marrow (BM) constitutes a microenvironment required for differentiation. Bone marrow mesenchymal stromal cells (BM-MSCs) strongly support MM cell growth, by producing a high level of Interleukin-6 (IL-6), a major MM cell growth factor. BM-MSCs also support osteoclastogenesis and angiogenesis. Previous studies have suggested that the direct (VLA-4, VCAM-1, CD44, VLA-5, LFA-1, syndecan-1,…) and indirect interactions (soluble factors) between MM plasma cells and BM-MSCs result in constitutive abnormalities in BM-MSCs. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4620

Platform:

GPL570

7 Samples

Download data: CEL

Analysis of bone marrow mesenchymal stromal cells (BM-MSCs) from untreated multiple myeloma (MM) patients. In MM, BM-MSCs support myeloma cell growth. Results provide insight into the molecular mechanisms underlying constitutive abnormalities in MM BM-MSCs relative to healthy donors’ BM-MSCs.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 disease state sets

Platform:

GPL570

Series:

GSE36474

7 Samples

Download data: CEL