GEO DataSets

(Submitter supplied) Melioidosis, caused by Gram negative bacteria Burkholderia pseudomallei, is a major type of community-acquired septicemia in Southeast Asia and Northern Australia with high mortality and morbidity rate. More accurate and rapid diagnosis is needed for improving the management of septicemic melioidosis. We previously identified 37-gene candidate signature to distinguish septicemic melioidosis from sepsis due to other pathogens. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

138 Samples

Download data: TXT

(Submitter supplied) Melioidosis is a severe infectious disease caused by Burkholderia pseudomallei, a gram-negative bacillus classified by the NIAID as a category B priority agent. Septicemia is the most common presentation of the disease with 40% mortality rate even with appropriate treatments. Faster diagnostic procedures are required to improve therapeutic response and survival rates. We have used microarray technology to generate genome-wide transcriptional profiles (>48,000 transcripts) of whole blood obtained from patients with septicemic melioidosis (n=32), patients with sepsis caused by other pathogens (n=31), and uninfected controls (n=29). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platforms:

GPL6947 GPL6106

106 Samples

Download data: XLS

(Submitter supplied) The study population consisted of 30 melioidosis cases, 10 healthy controls and 10 sepsis cases caused by other pathogens. Total RNA was extracted from peripheral blood mononuclear cells (PBMC’s) of study subjects. Gene expression profiles of 25 gene targets including 19 immune response genes and 6 epigenetic factors were analyzed by real time quantitative polymerase chain reaction (RT-qPCR).

Organism:

Homo sapiens

Type:

Expression profiling by RT-PCR

Platform:

GPL24545

50 Samples

Download data: XLSX

(Submitter supplied) Many microbial pathogens express specific virulence traits at distinct growth phases. To investigate the molecular pathways linking bacterial growth to pathogenicity, we characterized the genome-wide growth transcriptome of the tropical pathogen Burkholderia pseudomallei (Bp), the causative agent of melioidosis. Using fine-scale sampling, approximately 17% of all Bp genes were found to display regulated expression during growth, manifested primarily as discrete waves of gene expression tightly associated with distinct growth phases and transition points. more...

Organism:

Burkholderia pseudomallei K96243

Type:

Expression profiling by array

Dataset:

GDS2365

Platform:

GPL4078

47 Samples

Download data: GPR

Analysis of Burkholderia pseudomallei at the early, log, and stationary phases of growth. B. pseudomallei causes melioiosis. Results provide insight into the molecular pathways that link growth phase to pathogenicity.

Organism:

Burkholderia pseudomallei K96243

Type:

Expression profiling by array, log2 ratio, 4 other, 47 time sets

Platform:

GPL4078

Series:

GSE5495

47 Samples

Download data: GPR

(Submitter supplied) Natural isolates of Burkholderia pseudomallei (Bp), the causative agent of melioidosis, are known to exhibit diverse phenotypic traits, suggesting significant intraspecies genetic heterogeneity. Using whole-genome Bp microarrays, we experimentally mapped patterns of large-scale genomic variation in 93 South East Asian clinical, environmental, and animal Bp isolates. 14% of the reference Bp K96243 genome was variably present across the strain panel, more than double previous estimates, and both hypothetical proteins and paralogous gene pairs (PGPs) were significantly over-represented in the set of strain-variable genes. more...

Organism:

Burkholderia pseudomallei; Burkholderia pseudomallei K96243

Type:

Genome variation profiling by array

Platform:

GPL6073

93 Samples

Download data: GPR

(Submitter supplied) We report a transcriptional response in human OECs that encompasses multiple innate immune networks not previously associated with these cells. Major pathways included immune cell trafficking, and differential cytokine production

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

24 Samples

Download data: XLSX

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

225 Samples

Download data

(Submitter supplied) The wide array of molecules carried by plasma regulates critical immune functions and constitutes valuable biomarkers and therapeutic targets. In recent years the introduction of “systems approaches” has provided investigators with powerful means for assessing immune responses in patient samples on a global scale. However, while the use of genome-wide profiling technologies has become widespread, measuring the plasma proteome still presents considerable challenges. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

49 Samples

Download data: TXT

(Submitter supplied) The wide array of molecules carried by plasma regulates critical immune functions and constitutes valuable biomarkers and therapeutic targets. In recent years the introduction of “systems approaches” has provided investigators with powerful means for assessing immune responses in patient samples on a global scale. However, while the use of genome-wide profiling technologies has become widespread, measuring the plasma proteome still presents considerable challenges. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

40 Samples

Download data: TXT

(Submitter supplied) The wide array of molecules carried by plasma regulates critical immune functions and constitutes valuable biomarkers and therapeutic targets. In recent years the introduction of “systems approaches” has provided investigators with powerful means for assessing immune responses in patient samples on a global scale. However, while the use of genome-wide profiling technologies has become widespread, measuring the plasma proteome still presents considerable challenges. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

40 Samples

Download data: TXT

(Submitter supplied) The wide array of molecules carried by plasma regulates critical immune functions and constitutes valuable biomarkers and therapeutic targets. In recent years the introduction of “systems approaches” has provided investigators with powerful means for assessing immune responses in patient samples on a global scale. However, while the use of genome-wide profiling technologies has become widespread, measuring the plasma proteome still presents considerable challenges. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

40 Samples

Download data: TXT

(Submitter supplied) Bacterial transcriptomes are dynamic, context-specific and condition-dependent. Infection by the soil bacterium, Burkholderia pseudomallei, causes melioidosis, an often fatal infectious disease of humans and animals. Possessing a large multi-chromosomal genome, B. pseudomallei is able to persist and survive in a multitude of environments. To obtain a comprehensive overview of B. pseudomallei expressed transcripts, we initiated whole-genome transcriptome profiling covering a broad spectrum of conditions and exposures – a so-called “condition compendium”. more...

Organism:

Burkholderia pseudomallei K96243; Burkholderia pseudomallei; Burkholderia pseudomallei Bp22

Type:

Expression profiling by genome tiling array

Platform:

GPL16432

332 Samples

Download data: PAIR

(Submitter supplied) To identify signature genes that help distinguish (1) sepsis from non-infectious causes of systemic inflammatory response syndrome, (2) between Gram-positive and Gram-negative sepsis. Keywords: Class prediction study

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

70 Samples

Download data: CEL

(Submitter supplied) Sarcoidosis, a systemic granulomatous syndrome invariably affecting the lung, typically spontaneously remits but in ~20% of cases progresses with severe lung dysfunction or cardiac and neurologic involvement (complicated sarcoidosis). Unfortunately, current biomarkers fail to distinguish patients with remitting (uncomplicated) sarcoidosis from other fibrotic lung disorders, and fail to identify individuals at risk for complicated sarcoidosis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5175

74 Samples

Download data: CEL

(Submitter supplied) Sepsis is defined as a systemic inflammatory response secondary to a proven or suspected infection. Mechanisms governing this inflammatory response have been shown to be complex and dynamic, involving cross-talking among diverse signaling pathways. However, current knowledge on mechanisms underlying sepsis is far from providing a complete picture of the syndrome, justifying additional efforts that might add to this scenario. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

23 Samples

Download data: TXT

(Submitter supplied) Objective: It is unclear whether the host response of gram-positive sepsis differs from gram-negative sepsis at a transcriptome level. Using microarray technology, we compared the gene-expression profiles of gram-positive sepsis and gram-negative sepsis in critically ill patients. Design: A prospective cross-sectional study. Setting: A 20-bed general intensive care unit of a tertiary referral hospital. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3085

Platform:

GPL4274

72 Samples

Download data: GPR

(Submitter supplied) To examine whether gene-expression profiling of circulating leukocytes is different between patients with sepsis as compared to controls. The signature genes identified will then be used to develop a prediction model to assist the diagnosis of sepsis. Keywords: Prediction study

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2808

Platform:

GPL4274

94 Samples

Download data: GPR

Analysis of leukocytes from patients with gram-positive or gram negative sepsis. Results provide insight into the specificity of the host response to gram-positive and gram negative sepsis.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 4 disease state sets

Platform:

GPL4274

Series:

GSE6535

72 Samples

Download data: GPR

Analysis of neutrophils from critically ill patients with sepsis. Results used to define a gene expression signature for sepsis and provides insight into the host response to sepsis.

Organism:

Homo sapiens

Type:

Expression profiling by array, log ratio, 2 disease state, 2 protocol sets

Platform:

GPL4274

Series:

GSE5772

94 Samples

Download data: GPR