GEO DataSets

(Submitter supplied) Our study has demonstrated that significant number of differential genes in SLE was involved in IFN, TLR signaling pathways and inflammatory cytokines. The enrichment of differential genes has been associated with aberrant DNA methylation, which may be relevant to the pathogenesis of SLE. Our observations laid the groundwork for further diagnostic and mechanistic studies of SLE and LN.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL10558

55 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Homo sapiens

Type:

Expression profiling by array; Methylation profiling by genome tiling array

Platforms:

GPL13534 GPL10558

110 Samples

Download data

(Submitter supplied) Epigenetic alternations in addition to genetic factors are important contributors to the pathogenesis of Systemic Lupus Erythematosus (SLE). Recent studies revealed that aberrant changes in DNA methylation occur in SLE patients, and potentially contributes to the pathogenesis. Using genome-wide DNA methylation microarray, the Illumina Infinium HumanMethylation450 BeadChip, we compared the DNA methylation level of white blood cells between Chinese female SLE patients with that of healthy controls. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL13534

22 Samples

Download data: TXT

(Submitter supplied) Epienome-wide DNA methylation profiling of systemic lupus erythematosus (SLE). The Illumina HumanMethylation450K Beadchip was used to obtain DNA methylation profiles across approximately 450,000 CpGs in normal human blood samples from females. Samples included 33 non-SLE female patients (control) and 57 SLE female patients. SLE patients:- Ethnicity included 39 African americans and 18 European Americans. more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL13534

90 Samples

Download data: IDAT

(Submitter supplied) Objective: Systemic lupus erythematosus (SLE) has limited monozygotic (MZ) twin concordance, implying a role for other pathogenic factors than genetic variation, such as epigenetic changes. Using the disease discordant twin model, we investigated genome-wide DNA methylation changes in sorted CD4+ T-cells, monocytes, granulocytes and B-cells in twin pairs with at least one SLE-affected twin. Methods: Peripheral blood from 15 SLE twin pairs (six MZ, nine dizygotic (DZ)) was processed using gradient density centrifugation for the granulocyte fraction. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL13534

104 Samples

Download data: CSV, IDAT

(Submitter supplied) SLE patients are always with various disease manifestation. Various cytokines are pointed interacting and playing pathological roles in SLE although the etiopathology is still obscure. In this study, we aimed to investigate the effects of cytokine interactions in the immune response of SLE patients. Overexpressed interferon-inducible(IFI) genes were confirmed in peripheral blood from SLE patients. Using network-based analysis on the immune response-related genes, several networks including cytokines such as TNF and IFN-γ, or beta-estradiol(E2), were constructed. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1291

17 Samples

Download data: TXT

(Submitter supplied) Systemic lupus erythematosus (SLE) is a chronic-relapsing autoimmune disease of incompletely understood etiology. Recent evidence strongly supports an epigenetic contribution to the pathogenesis of lupus. To understand the extent and nature of dysregulated DNA methylation in lupus T cells, we performed a genome-wide DNA methylation study in CD4+ T cells from 12 lupus patients and 12 normal healthy controls. more...

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL8490

23 Samples

Download data: TXT

(Submitter supplied) Male patients with systemic lupus erythematosus (SLE) experience severe disease compared to female patients, despite the disease being more prevalent in females. For the time, we compared genome-wide differential methylation in CD4+ T cells between male (n=12) and female (n=10) SLE patients.

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL21145

22 Samples

Download data: IDAT, TXT, XLSX

(Submitter supplied) Recent studies have shown that alterations in the function of dendritic cells (DCs) are involved in the pathogenesis of systemic lupus erythematosus (SLE). However, the role of DCs participating in SLE remains unclear. We profiled the microRNAs (miRNAs) of LPS-stimulated DCs in SLE patients and found diffentially expressed miRNAs in DCs of such group patients.

Organism:

Homo sapiens

Type:

Non-coding RNA profiling by array

Platform:

GPL18402

10 Samples

Download data: TXT

(Submitter supplied) Systemic lupus erythermatosus (SLE) is a complex autoimmune disease, and epigenetic study is promissing for illustrating the mechanisms of SLE pathogenesis. Assay for transposase accessible chromatin in single cells sequencing (scATAC-seq) shows priority to trackle this barrier. Thus, scATAC-seq was applied to difine the landscape of active regulatory DNA in systemic lupus erythermatosus (SLE) at single cell resolusion. more...

Organism:

Homo sapiens

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL23227

2 Samples

Download data: BED, MTX, TSV

(Submitter supplied) Systemic lupus erythematosus (SLE) is a chronic relapsing autoimmune disease characterized by the production of autoantibodies and multiple organ involvement. In this study, we investigated genome-wide DNA methylation changes in the CD8+ T cells from 8 pairs of lupus patients compared to age, sex, and ethnicity matched healthy controls.

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL23976

16 Samples

Download data: IDAT, TXT

(Submitter supplied) Antiphospholipid syndrome (APS) is a systemic autoimmune disease characterized by thromboembolic events and pregnancy loss. We sought to characterize the DNA methylation profile of primary APS in comparison to healthy controls and individuals with SLE.

Organism:

Homo sapiens

Type:

Methylation profiling by array

Platform:

GPL13534

22 Samples

Download data: IDAT, TXT

(Submitter supplied) This study demonstrates quantitative and qualitative differences between type I IFN signatures in autoimmunity and viral infection using purified CD4pos T cells and CD16pos- and CD16neg-monocyte subsets. We were able to discriminate between cell-specific viral response signatures and the pathogenically amplified IFN signatures observed in autoimmunity. The differences were of both a qualitative and quantitative nature, as the signatures in the patients with SLE were characterized by much more complexly compiled gene patterns with increased absolute gene expression levels.

Organism:

Homo sapiens

Type:

Expression profiling by array

Datasets:

GDS4888 GDS4889 GDS4890

Platform:

GPL570

36 Samples

Download data: CEL, CHP

Analysis of CD16+ monocytes from SLE patients and YFV-immunized healthy donors. The YFV immunization can be regarded as a real viral infection, based on clinical/serological manifestations. Results provide insight into differences in type I interferon responses in autoimmunity and viral infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 7 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE51997

10 Samples

Download data: CEL, CHP

Analysis of CD16- monocytes from SLE patients and YFV-immunized healthy donors. The YFV immunization can be regarded as a real viral infection, based on clinical/serological manifestations. Results provide insight into differences in type I interferon responses in autoimmunity and viral infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 8 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE51997

12 Samples

Download data: CEL, CHP

Analysis of CD4+ T cells from SLE patients and YFV-immunized healthy donors. The YFV immunization can be regarded as a real viral infection, based on clinical/serological manifestations. Results provide insight into differences in type I interferon responses in autoimmunity and viral infection.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 disease state, 10 individual, 3 protocol sets

Platform:

GPL570

Series:

GSE51997

14 Samples

Download data: CEL, CHP

(Submitter supplied) Epigenetics may play a central, but yet unexplored, role in the profound changes that the maternal immune system undergoes during pregnancy. We investigated changes in the methylome in isolated circulating CD4+ T cells in non-pregnant and pregnant women, during the 1st and 2nd trimester, using the Illumina Infinium Human Methylation 450K array, and explored how these changes were related to autoimmune diseases that are known to be affected during pregnancy. more...

Organism:

Homo sapiens

Type:

Methylation profiling by genome tiling array

Platform:

GPL13534

35 Samples

Download data: IDAT

(Submitter supplied) Gene expression profile studies have identified an interferon signature in whole blood or mononuclear cell samples from patients with systemic lupus erythematosus. This study was designed to determine whether specific lymphocyte and myeloid subsets freshly isolated from the blood of systemic lupus erythematosus patients demonstrated unique gene expression profiles compared to subsets isolated from healthy controls. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS4185

Platform:

GPL96

67 Samples

Download data: CEL

Analysis of freshly isolated lymphocyte (CD4+ T cells and CD19+ B cells) and CD33+ myeloid subsets from the blood of Systemic Lupus Erythematosus (SLE) patients. Results provide insight into the molecular mechanisms underlying SLE pathogenesis.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 cell type, 2 disease state sets

Platform:

GPL96

Series:

GSE10325

67 Samples

Download data: CEL

(Submitter supplied) We performed RNA-seq experiments to modulate levels of XIST RNA. We inhibited XIST activity in REH lymphocytic cells via knockdown of XIST expression by short hairpin (sh) RNA. We confirmed that the RNA level of XIST was greatly reduced in shRNA-XIST cells by both RNA-seq and qPCR analyses, relative to cells transfected with a scrambled shRNA.

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL18573

2 Samples

Download data: TXT