Similar studies for GEO DataSets (Select 200089416) - GEO DataSets

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Items: 20

Select item 2000894161.

ATAC-seq analysis in 5F cells and AGM cells with EZH1 depletion

(Submitter supplied) Blood develops in distinct stages. Haematopoietic progenitors in the embryo manifest restricted differentiation potential relative to definitive haematopoietic stem cells in adult bone marrow, which support lifelong multilineage haematopoiesis. To identify regulators of embryonic haematopoiesis, we screened chromatin modifiers and identified the Polycomb group protein EZH1 as a barrier to multilineage potential from pluripotent stem cells (PSCs). more...

Organism:: Homo sapiens; Mus musculus

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platforms:: GPL19057 GPL18573
7 Samples

Download data: BED, WIG

Series

Accession:: GSE89416

ID:: 200089416

PubMed Full text in PMC Similar studies SRA Run Selector

Select item 2000894182.

EZH1 as a key epigenetic barrier to definitive haematopoiesis during embryonic development

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

4 related Platforms
58 Samples

Download data: BED, TXT, WIG

Series

Accession:: GSE89418

ID:: 200089418

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Select item 2000894173.

ChIP-seq analysis of EZH1, H3K4me3 and H3K27me3 in 5F cells

Organism:: Homo sapiens

Type:: Genome binding/occupancy profiling by high throughput sequencing

Platform:: GPL18573
15 Samples

Download data: BED, WIG

Series

Accession:: GSE89417

ID:: 200089417

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Select item 2000894154.

RNA-seq analysis of EZH1 knockdown in 5F cells, or EZH1 heterozygous and homozygous knockout YS and AGM cells

Organism:: Mus musculus; Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL17021 GPL16791
36 Samples

Download data: TXT

Series

Accession:: GSE89415

ID:: 200089415

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Select item 2001714575.

Multipotent progenitors and hematopoietic stem cells arise independently from hemogenic endothelium in the mouse embryo

(Submitter supplied) During embryogenesis, waves of hematopoietic progenitors develop from hemogenic endothelium (HE) prior to the emergence of self-renewing hematopoietic stem cells (HSC). Although previous studies have shown that yolk sac-derived erythromyeloid progenitors and HSC emerge from distinct populations of HE, it remains unknown whether the earliest lymphoid-competent progenitors, multipotent progenitors, and HSC originate from common HE. more...

Organism:: Mus musculus

Type:: Expression profiling by high throughput sequencing

Platform:: GPL19057
2 Samples

Download data: MTX, RDS, TSV

Series

Accession:: GSE171457

ID:: 200171457

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Select item 2001629506.

Mapping Human Hematopoietic Stem Cells From Hemogenic Endothelium To Birth

(Submitter supplied) Human hematopoietic stem cell (HSC) ontogeny is poorly defined due to the inability to identify HSCs as they emerge and mature in different hematopoietic sites. We created a single-cell transcriptome map of human hematopoietic tissues from 1st trimester to birth and found that HSC signature RUNX1+HOXA9+MLLT3+MECOM+HLF+SPINK2+ distinguishes HSCs from progenitors throughout gestation. In addition to the AGM (aorta-gonad-mesonephros) region, nascent HSCs populated the placenta and yolk sac before colonizing the liver at 6 weeks. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24676
28 Samples

Download data: CSV, PNG, TAR

Series

Accession:: GSE162950

ID:: 200162950

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Select item 2000576627.

Reprogramming of Endothelium Into Hematopoietic Progenitors by Defined Factors and Vascular Induction

(Submitter supplied) Generation of abundant engraftable hematopoietic cells from autologous tissues promises new therapies for hematologic diseases. Differentiation of pluripotent stem cells into hematopoietic cells results in emergence of cells that have poor engraftment potential. To circumvent this hurdle, we have devised a vascular niche model to phenocopy the developmental microenvironment of hemogenic cells thereby enabling direct transcriptional reprogramming of human endothelial cells (ECs) into hematopoietic cells. more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL11154
16 Samples

Download data: TXT

Series

Accession:: GSE57662

ID:: 200057662

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Select item 2001739728.

Differential Ezh1/2 regulation of hemogenic fate and hematopoietic stem and progenitor cell formation from arterial endothelium

(Submitter supplied) Across species, hematopoietic stem and progenitor cells (HSPCs) arise during embryogenesis from a specialized arterial population, termed hemogenic endothelium. Here, we describe a mechanistic role for the epigenetic regulator, Enhancer of zeste homolog-1 (Ezh1) in vertebrate HSPC production via regulation of hemogenic commitment. Loss of ezh1 in zebrafish embryos favored acquisition of hemogenic (gata2b) and HSPC (runx1) fate at the expense of the arterial program (ephrinb2a, dll4). more...

Organism:: Danio rerio

Type:: Expression profiling by high throughput sequencing

Platform:: GPL24995
6 Samples

Download data: MTX, RDS, TSV

Series

Accession:: GSE173972

ID:: 200173972

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Select item 2000362889.

Expression data from LSK cells from mice with knock-out of Ezh1 versus LSK control

(Submitter supplied) Ezh1 is a protein member of PRC2. Ezh1 has been described as a functional repressor gene, such as its homologous Ezh2. We are investigating the role of Ezh1 in hematopoietic stem cells, aging, self-renewal and differentiation. We used microarrays to detail the global program of gene expression in LSK cells from mice with knocked-down expression of Ezh1.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL10787
4 Samples

Download data: TXT

Series

Accession:: GSE36288

ID:: 200036288

Select item 20002311010.

DNA methylation data from mouse hematopoietic progenitors

(Submitter supplied) Genome-wide DNA methylation was studied to determine the methylome map of lymphoid and myeoloid commitment from hematopoietic progenitors We used custom Nimblegen microarrays to determine the genome-wide DNA methylation in FACs purified mouse hematopoietic progeniors

Organism:: Mus musculus

Type:: Methylation profiling by genome tiling array

Platforms:: GPL10680 GPL10683
35 Samples

Download data: XYS

Series

Accession:: GSE23110

ID:: 200023110

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Select item 20002024411.

A comprehensive methylome map of lineage commitment from hematopoietic progenitors

(Submitter supplied) Epigenetic modifications must underlie lineage-specific differentiation since terminally differentiated cells express tissue-specific genes, but their DNA sequence is unchanged. Hematopoiesis provides a well-defined model of progressive differentiation in which to study the role of epigenetic modifications in cell fate decisions. Multi-potent progenitors (MPPs) can differentiate into all blood cell lineages, while downstream progenitors commit to either myeloerythroid or lymphoid lineages. more...

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
26 Samples

Download data: CEL

Series

Accession:: GSE20244

ID:: 200020244

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Select item 20015967212.

Recapitulative hematopoietic development of human pluripotent stem cells in the absence of exogenous cytokines

(Submitter supplied) We tested whether the sustained hematopoietic differentiation of hPSCs can be achieved in defined cell culture conditions without addition of hematopoietic cytokines. Here we show that the endogenous stimuli were sufficient to induce a robust generation of clonogenic hematopoietic progenitors, maturation of blood cells, emergence of the definitive cell lineages, and progenitors that were phenotypically identical to early human embryonic hematopoietic stem cells (HSCs). more...

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platforms:: GPL16791 GPL20795 GPL21290
34 Samples

Download data: TSV

Series

Accession:: GSE159672

ID:: 200159672

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Select item 20005305613.

Geminin regulates self-renewal and fate commitment decisions in fetal hematopoietic stem cells.

(Submitter supplied) Conditional deletion of Geminin from the entire hematopoietic compartment using Vav1:iCre mice led to defective hematopoiesis/dyserythropoiesis in E15.5 mouse embryos. The present data set includes data from lineage-negative cells isolated from homogenized livers that were dissected from E15.5.dpc embryos. The two conditions compared were wild-type versus Geminin-KO Lin- cells. The cells were collected from littermates.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL6246
6 Samples

Download data: CEL

Series

Accession:: GSE53056

ID:: 200053056

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Select item 20001979614.

Expression data from Bmi1-null c-Kit+Sca-1+Lineage marker- (KSL) hematopoietic stem/progenitor cells

(Submitter supplied) Bmi1 is a component of polycomb repressive complex 1 and its role in the inheritance of the stemness of adult somatic stem cells has been well characterized. Bmi1 maintains the self-renewal capacity of adult stem cells, at least partially, by repressing the Ink4a/Arf locus that encodes a cyclin-dependent kinase inhibitor, p16Ink4a, and a tumor suppressor, p19Arf 14. Deletion of both Ink4a and Arf in Bmi1-deficient mice substantially restored the defective self-renewal capacity of HSCs and neural stem cells.

Organism:: Mus musculus

Type:: Expression profiling by array

Platform:: GPL1261
7 Samples

Download data: CEL, CHP

Series

Accession:: GSE19796

ID:: 200019796

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Select item 20008511215.

Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells via Transcription Factor Conversion of Hemogenic Endothelium

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
7 Samples

Download data: TXT

Series

Accession:: GSE85112

ID:: 200085112

PubMed Full text in PMC Similar studies

Select item 20008511116.

inDrop single cell RNA-seq of hematopoietic cells derived from human pluripotent stem cells

(Submitter supplied) We performed morphogen-directed differentiation of human PSCs into HE followed by combinatorial screening of 26 candidate HSC-specifying TFs for the potential to promote hematopoietic engraftment in irradiated immune deficient murine hosts. We recovered seven TFs (ERG, HOXA5, HOXA9, HOXA10, LCOR, RUNX1, SPI1) that together were sufficient to convert HE into hematopoietic stem and progenitor cells (HSPCs) that engraft primary and secondary murine recipients

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
2 Samples

Download data: TXT

Series

Accession:: GSE85111

ID:: 200085111

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Select item 20008371917.

Transcriptome profiling of hematopoietic cells derived from human pluripotent stem cells

Organism:: Homo sapiens

Type:: Expression profiling by high throughput sequencing

Platform:: GPL18573
5 Samples

Download data: TXT

Series

Accession:: GSE83719

ID:: 200083719

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Select item 20018221318.

Single-cell RNA sequencing analysis of transgenic zebrafish embryo/larvae

(Submitter supplied) The purpose of the experiment was to define the heterogeneity of hematopoietic stem and progenitor cells (HSPC) at emergence and initial maturation using scRNA-Seq of enriched blood populations from transgenic fluorescent zebrafish (30 and 52 hpf). Results provide insight into the different HSPC populations in heamtopoietic development.

Organism:: Danio rerio

Type:: Expression profiling by high throughput sequencing

Platform:: GPL21741
6 Samples

Download data: LOOM, MTX, TSV

Series

Accession:: GSE182213

ID:: 200182213

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Select item 20009074219.

Clonal analysis of lineage fate in unperturbed hematopoiesis

(Submitter supplied) The classical tenet of hematopoiesis posits well-accepted lineage trees that arise from progressively restricted oligopotent and unipotent progenitor populations. However, because fate in hematopoiesis has mostly been studied in the context of transplantation, it is unclear whether these lineage branches and such proposed oligopotent progenitors exist in an unperturbed hematopoietic system. Here, we utilize endogenous transposon tagging to trace the fate of thousands of progenitors and stem cells over time to re-evaluate these dogmas. more...