GEO DataSets

(Submitter supplied) Comparison of transcriptome data in fed and fasted conditions of zebrafish (Danio rerio) and rainbow trout (Oncorhynchus mykiss) anterior intestine using the serial analysis of gene expression (SAGE) method was done in a strategy to identify key regulated genes at the transcript level and to allow the identification of activated/deactivated pathways after feeding.

Organism:

Oncorhynchus mykiss; Danio rerio

Type:

Expression profiling by SAGE

Platforms:

GPL3209 GPL13690 GPL13689

4 Samples

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(Submitter supplied) The experiments were designed to gain insight into the gene expression of conventional and unconventional murine T cell subsets in the steady state and upon activation

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

5 Samples

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(Submitter supplied) Despite a considerable literature concerning the molecular pathogenesis of pituitary tumors, the mechanisms of pituitary tumors development and progression remain unknown. Four SAGE cDNA libraries were constructed using a pool of mRNA obtained from five GH-, two ACTH-secreting, and four non secreting pituitary tumors (NS), and three normal pituitaries from patients who had accidental death, using I-SAGE kit (Invitrogen). more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

4 Samples

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(Submitter supplied) Oral susceptibility of Aedes aegypti mosquitoes to dengue viruses varies between different Aedes species and strains. However, the midgut-specific transcriptional profile that may produce this variation is presently obscure and was the subject of our investigation. The variation in active expression between dengue-2 susceptible (SUS) and refractory (REF) mosquitoes was investigated during the first critical 96 hours after infection Transcriptional profiles were mined from respective guts using the serial analysis of gene expression technique (SAGE) and libraries constructed from midguts obtained from mosquitoes that received a dengue-2 infected blood meal (DENV-2), a non infected blood meal (naive) or a 5% sucrose meal (SM). more...

Organism:

Aedes aegypti

Type:

Expression profiling by SAGE

Platform:

GPL8061

5 Samples

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(Submitter supplied) HTLV-1 is the etiologic agent of HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP). CD8+ T cells may contribute to the protection or development of HAM/TSP. In this study we used SAGE to assess gene expression profiles of CD8+ T cells isolated from HTLV-1 asymptomatic carriers (HAC) and from HAM/TSP patients, in order to identify genes involved in the HAM/TSP development. Analysis of SAGE was conducted by pooling samples according to clinical status. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

2 Samples

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(Submitter supplied) Oral epithelial dysplasias are believed to progress through a series of histopathological stages; from mild to severe dysplasia, to carcinoma in situ, and finally to invasive OSCC. Underlying this change in histopathological grade are gross chromosome alterations and changes in gene expression of both protein-coding genes and non-coding RNAs. Recent papers have described associations of aberrant expression of microRNAs, one class of non-coding RNAs, with oral cancer. more...

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

10 Samples

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(Submitter supplied) To identify functions that distinguish the posterior and median cells producing fibroin and sericin in the silk gland of Bombyx mori, serial analysis of gene expression (SAGE) profiles from both silk gland regions were analyzed and compared. The construction of a B. mori reference tag collection extracted from a set of 38000 Bombyx EST sequenced from the 3’ side, helped annotating the SAGE libraries. more...

Organism:

Bombyx mori

Type:

Expression profiling by SAGE

Platform:

GPL2095

2 Samples

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(Submitter supplied) Arabidopsis thaliana (Col-4) aerial tissue collected from 14-day old plants. Grown under sterile conditions at 22 degrees Celsius and 125 uE light on 0.5xMS media.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by SAGE

Platform:

GPL25

1 Sample

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(Submitter supplied) Dark-grown maize seedlings with primary roots 12-20 mm in length were transplanted to high (-0.03 MPa) water potential vermiculite. About 1,000 roots were collected. Each root was divided into 4 segments (distances are from the junction of the root apex and root cap): segment 1, 0-3 mm plus the root cap; segment 2, 3-7 mm; segment 3, 7-12 mm; segment 4, 12-20 mm. Details of the conditions and nutrient modifications have been described before (Sharp et al., 1988; Spollen et al, 2000). more...

Organism:

Zea mays

Type:

Expression profiling by SAGE

Platform:

GPL1244

1 Sample

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(Submitter supplied) Tissue from macroscopically healthy parts of nephrectomized kidneys. Endothelial cells were purified from the cell outgrowth of glomeruli twice using CD31 magnetic beads and cultured on fibronectin coated surface. Cells were not passaged more than eight times and stained with vWf, CD31, and after stimulation with TNF alpha also with CD62E/P.

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) Cardiac fibroblasts were isolated from 3-month old C57BL/6 mice. RNA was prepared from quiescent, serum-starved cardiac fibroblasts in passage 15. A total number of 118654 tags was sequenced. After removal of duplicate ditags and linker-derived sequences 110169 tags remained.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

1 Sample

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(Submitter supplied) This library was constructed from mRNA derived from an eight month old female with type 2 Gaucher disease according to the SAGE protocol of V. E. Velculescu et al. (1995), in the laboratory of R. L. Proia (NIDDK, NIH).

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) This library was generated from normal human thyroid tissue collected at routine autopsy. Standard SAGE protocol was used (Velculescu et al. Science 1995). Data from this library was published in: 'Pauws E, Moreno JC, Tijssen M, Baas F, de Vijlder JJ, Ris-Stalpers C. J Clin Endocrinol Metab. 2000 May;85(5):1923-7' Data was generated and analyzed at the laboratory of Pediatric Endocrinology, Academic Medial Center, Amsterdam, The Netherlands.

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) Uterine cervix normal SAGE, CGAP non-normalized SAGE library, bulk method , HPV negative

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

1 Sample

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(Submitter supplied) P23 C57BL6/J mouse cerebellum

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

1 Sample

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(Submitter supplied) SAGE profile of rat hippocampus mRNA from Wistar rats

Organism:

Rattus norvegicus

Type:

Expression profiling by SAGE

Platform:

GPL23

1 Sample

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(Submitter supplied) SAGE libraries from mouse

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

5 Samples

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(Submitter supplied) These libraries represent Cancer Genome Anatomy Project libraries, which are either produced through CGAP funding, or donated to CGAP. The Cancer Genome Anatomy Project (CGAP: http://cgap.nci.nih.gov) is an interdisciplinary program established and administered by the National Cancer Institute (NCI: http://www.nci.nih.gov) to generate the information and technological tools needed to decipher the molecular anatomy of the cancer cell.

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

3 Samples

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(Submitter supplied) To better understand the early events regulating lineage-specific hematopoietic differentiation, we analyzed the transcriptional profiles of CD34+ human hematopoietic stem and progenitor cells (HSPCs) subjected to differentiation stimulus.

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

3 Samples

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(Submitter supplied) Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis.

Organism:

Mus musculus

Type:

Expression profiling by SAGE

Platform:

GPL11

2 Samples

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