GEO DataSets

(Submitter supplied) The glycopeptide vancomycin is a drug of choice for the treatment of severe non-gastrointestinal infections with members of Bacillus cereus sensu lato. Recently, sporadic detection of vancomycin resistant phenotypes emerged, mostly for agar diffusion testing. The food packaging isolate BC70 displayed a pseudo-resistant phenotype for vancomycin in both Etest and disk diffusion. In this work, we used RNA-Seq on the nanopore platform to study differentially expressed genes in BC70 cells, which were able to actively move into the inhibition zone during vancomycin susceptibility testing using Etest and therefore appeared to be resistant. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34081

4 Samples

Download data: CSV

(Submitter supplied) To investigate the rapid adaptation mechanism of Bacillus thuringiensis in an alkaline environment, we have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between normal condition and alkaline condition.

Organism:

Bacillus thuringiensis

Type:

Expression profiling by array

Platform:

GPL17384

6 Samples

Download data: TXT

(Submitter supplied) Antibiotic resistance (AR) is one of the greatest threats to global health and is associated with higher treatment costs, longer hospital stays, and increased mortality. Current gold standard antibiotic susceptibility tests (AST) are dependent on organism growth rates resulting in prolonged diagnostic answers for slow growing organisms. Changes in the cellular transcriptome can be instantaneous in the presence of stressors such as antibiotic pressure. more...

Organism:

Bacillus anthracis; Yersinia pestis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL33703 GPL29781

48 Samples

Download data: XLSX

(Submitter supplied) The iron-binding glycoprotein lactoferrin exhibits anti-bacterial activity. To investigate the overall response of Bacillus cereus to lactoferrin, total transcriptome analyses were performed. Growth conditions were mMOD minimal medium, minimal medium plus 10 mg/ml lactoferrin, as well as minimal medium without iron source for comparison. Differential regulation of genes involved in iron acquisition as well as genes encoding enterotoxins and further virulence factors was of special interest.

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26265

6 Samples

Download data: GCT, GFF, TSV, TXT, XLSX

(Submitter supplied) We then performed gene expression profiling using data obtained from RNA-seq of 2 different cells at three time points.

Organism:

Bacillus thuringiensis serovar kurstaki str. HD73

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32767

18 Samples

Download data: TAB

(Submitter supplied) In preliminary experiments, the absence of anthrose in our genetic Sterne knockout was found to decrease pagA expression in BHI broth containing glucose (Fig 5A and B). This contrasted with the large spike in pagA expression when the Sterne antC mutant is grown in protein rich HIB media which does not contain sugars. When exogenous anthrose was added during growth in BHI broth, expression from PpagA increased in both WT (purple line in Fig 5A) and antC Sterne (purple line in Fig 5B). more...

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31186

12 Samples

Download data: XLSX

(Submitter supplied) The formation of endospores has always been considered as the unique mode of survival and transmission of sporulating Firmicutes due to the exceptional resistance and persistence of this bacterial cell form. In this study, we investigated the behavior of a bacterial population in the late stages of an infection as well as the characteristics of the Spo- bacterial form in the B. thuringiensis/Galleria mellonella infection model. more...

Organism:

Bacillus thuringiensis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32378

9 Samples

Download data: XLSX

(Submitter supplied) Background: The Bacillus cereus Sigma B (SigB) dependent general stress response is activated via the two-component RsbKY system, which involves a phosphate transfer from RsbK to RsbY. It has been hypothesized that the Hpr-like phosphocarrier protein (Bc1009) encoded by bc1009 in the SigB gene cluster may play a role in this transfer, thereby acting as a regulator of SigB activation. Alternatively, Bc1009 may be involved in the activation of a subset of SigB regulon members. more...

Organism:

Bacillus cereus ATCC 14579

Type:

Expression profiling by array

Platform:

GPL9493

16 Samples

Download data: TXT

(Submitter supplied) Transcriptome analyses after the transfer of pCER270-spec megaplasmid in B. cereus F4810-72 (previously cured for its plasmid), B. thuringiensis HD73, and B. weihenstephanensis KBAB6 strains. We determined differentially expressed genes in pCER270+ strains in comparison with pCER270- strains.

Organism:

Bacillus cereus; Bacillus thuringiensis; Bacillus mycoides

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL29834 GPL32378 GPL32936

36 Samples

Download data: TXT

(Submitter supplied) Bacillus anthracis causes anthrax infections in mammals. Large-scale mortality resulting from the intentional release of B. anthracis spores represents a potential bioterrorism threat. Inhalational anthrax almost invariably proceeds to fatal systemic infection, characterized by massive bacteremia. A better understanding of host-pathogen interactions is urgently needed for effective treatment of this lethal disease. more...

Organism:

Bacillus anthracis str. Sterne; Bacillus anthracis

Type:

Expression profiling by array

Platform:

GPL23273

4 Samples

Download data: MEV

(Submitter supplied) Bacillus cereus is the second leading cause of collective food poisoning in France. B. cereus is also associated with severe clinical infections leading to patient death in 10% of the cases. The emergence of B. cereus as a foodborne and opportunistic pathogen has intensified the need to distinguish strains of public health concern. In this work, by performing a screen on a large collection of B. cereus strains of varying pathogenic potential, we identified genetic determinants capable of discriminating B. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29834

45 Samples

Download data: CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL31186

20 Samples

Download data

(Submitter supplied) Formaldehyde cross-linking RNA immunoprecipitation coupled with illumina sequencing (fRIP-seq) was performed to pinpoint the direct RNA targets of KrrA using a FLAG-tagged KrrA construct that is driven by a constitutive promoter Plgt (pOS1.PlgtkrrA-FLAG).

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL31186

8 Samples

Download data: XLSX

(Submitter supplied) To uncover the effects of KrrA regulation on gene transcription and define the bacterial response imposed by this regulation, a transcriptomic study was carried out in which Bacillus anthracis krrA was compared to WT in two growth conditions: LB in the presence or absence of ‘205.

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31186

12 Samples

Download data: XLSX

(Submitter supplied) AtxA, the master virulence regulator of Bacillus anthracis, regulates the expression of three toxins that are required for the pathogenicity of Bacillus anthracis. Recent transcriptome analyses also showed that AtxA affects a large number of genes on both chromosome and plasmid, suggesting its role as a global regulator. Its mechanism of gene regulation nor binding target in vivo was, however, not well understood. more...

Organism:

Bacillus anthracis

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL29781 GPL29782

37 Samples

Download data: TXT

(Submitter supplied) Bacterial response to nitric oxide (NO) is of major importance for bacterial survival, as NO stress is a main actor of the eukaryote immune response. Several pathogenic bacteria have developed gene regulation systems involved in detoxification and in repairing the damages caused by NO. However, bacterial mechanisms of NO resistance are poorly described for Gram positive bacteria and especially Bacillus cereus. more...

Organism:

Bacillus cereus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29834

21 Samples

Download data: CSV

(Submitter supplied) Spores of Bacillales and Clostridiales species contain 100s of different mRNAs, and their major function in Bacillus subtilis is to provide ribonucleotides for new RNA synthesis when spores germinate. In new work, RNA was isolated from spores of five Bacillales and one Clostridioides species and relative spore mRNA levels were determined by RNA-seq. Determination of RNA levels in single spores allowed calculation of RNA nt/spore, and assuming mRNA is 3% of spore RNA allowed calculation that only ~6% of spore mRNAs were present at ≥ 1/spore. more...

Organism:

Bacillus atrophaeus ATCC 9372; Geobacillus stearothermophilus ATCC 12980; Bacillus thuringiensis str. Al Hakam; Bacillus subtilis; Clostridioides difficile 630; Priestia megaterium QM B1551

Type:

Expression profiling by high throughput sequencing

6 related Platforms

12 Samples

Download data: TXT

(Submitter supplied) Purpose: Small regulatory RNAs (sRNAs) are short transcripts that base-pair to mRNA targets or interact with regulatory proteins. sRNA function has been extensively studied in Gram-negative bacteria; comparatively less is known about sRNAs in Firmicutes. Here we investigate two sRNAs encoded by the virulence plasmid pXO1 of Bacillus anthracis, the causative agent of anthrax. We designated the sRNAs as as “XrrA” and “XrrB” (for pXO1-encoded regulatory RNA). more...

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28686

12 Samples

Download data: BIGWIG, TXT

(Submitter supplied) Purpose: Bacillus anthracis produces three regulators, AtxA, AcpA and AcpB, which control virulence gene transcription and belong to an emerging class of regulators termed ‘PCVRs’ (Phosphoenolpyruvate-dependent phosphotransferase regulation Domain-Containing Virulence Regulators). AtxA, named for its control of toxin gene expression, is the master virulence regulator and archetype PCVR. AcpA and AcpB are less well studied. more...

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28687

15 Samples

Download data: TDF, TXT

(Submitter supplied) The High Temperature Requirement (HtrA) chaperone/protease is involved in the stress-response of the anthrax-causing pathogen Bacillus anthracis. Resilience to oxidative stress is essential for manifestation of B. anthracis pathogenicity. We submitt transcriptome data sets detailing global gene expression in B. anthracis wild-type and htrA-disrupted strains following H2O2-induced oxidative stress.

Organism:

Bacillus anthracis str. Vollum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28578

14 Samples

Download data: TXT