GEO DataSets

(Submitter supplied) To identify chloroplastic H2O2-responseive genes, an estrogen-inducible RNAi method was used for silencing of tAPX, a H2O2-scavenging enzyme in chloroplasts. At 48 h after treatment with estrogen, the expression of tAPX was silenced in the IS-tAPX-19-23 plants, but not in the IS-GUS-2-17 plants (negative control), resulting a large change in gene expression. These genes are candidates for chloroplastic H2O2-responseive genes.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL7299

4 Samples

Download data: TXT

(Submitter supplied) We focused on Arabidopsis chloroplast ascorbate peroxidases (sAPX and tAPX) and proton gradient regulation 5 (PGR5) and used pgr5 single mutant, sapx tapx double mutant, and sapx tapx pgr5 triple mutant for RNA-seq analysis.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17970

12 Samples

Download data: XLSX

(Submitter supplied) Reduced glutathione (GSH) is required for cell cycle initiation and auxin-regulated root meristem development. Transcriptome profiling of the roots and shoots of the root meristemless 1 (rml1) mutant, which has about 3% of the wild type GSH, revealed a divergent auxin and strigolactone response linked to the arrest of the cell cycle.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL14926

8 Samples

Download data: CEL

(Submitter supplied) Plants adapt to abiotic and biotic stresses by activating abscisic acid-mediated (ABA) abiotic stress-responsive and salicylic acid-(SA) or jasmonic acid-mediated (JA) biotic stress-responsive pathways, respectively. Although the abiotic stress-responsive pathway interacts antagonistically with the biotic stress-responsive pathways, the mechanisms that regulate these pathways remain largely unknown. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL7299

8 Samples

Download data: TXT

(Submitter supplied) We used the flu mutant of Arabidopsis and a transgenic line that overexpresses the thylakoid-bound ascorbate peroxidase (tAPX) to address the interactions between different reactive oxygen species (ROS) signaling pathways. The conditional flu mutant of Arabidopsis accumulates excess protochlorophyllide in the dark within chloroplast membranes that upon illumination acts as a photosensitizer and generates singlet oxygen (1O2). more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

8 Samples

Download data: CEL, CHP

(Submitter supplied) Shortly after the release of singlet oxygen (1O2), drastic changes in nuclear gene expression occur in the conditional flu mutant of Arabidopsis that reveal a rapid transfer of signals from the plastid to the nucleus. In contrast to retrograde control of nuclear gene expression by plastid signals described earlier, the primary effect of 1O2 generation in the flu mutant is not the control of chloroplast biogenesis but the activation of a broad range of signaling pathways known to be involved in biotic and abiotic stress responses. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

10 Samples

Download data: CEL, CHP

(Submitter supplied) Cold stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified a DEAD box RNA helicase, RCF1 (Regulator of CBF gene expression 1) that controls pre-mRNA splicing of cold-stress-responsive genes including positive and negative regulators of CBF genes. We used whole genome tiling array analysis under cold stress to identify transcripts which are mis-spliced/intron-retained in rcf1-1.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by genome tiling array

Platform:

GPL10977

6 Samples

Download data: CEL, TXT

(Submitter supplied) Cold stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified a DEAD box RNA helicase, RCF1 (Regulator of CBF gene expression 1) that controls pre-mRNA splicing of cold-stress-responsive genes including positive and negative regulators of CBF genes. We used whole genome tiling array analysis under normal growth conditions to identify transcripts which are mis-spliced/intron-retained in rcf1-1.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by genome tiling array

Platform:

GPL10977

6 Samples

Download data: CEL, TXT

(Submitter supplied) Cold stress is one of the most severe environmental conditions which cause huge losses in crop production worldwide. We identified an essential regulator of cold-responsive genes and used the Affymetrix whole-genome arrays to define downstream targets of this important protein. We used the microarrays to reveal the effect of rcf1-1 mutation on global gene expression with or without cold stress at 4°C. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

12 Samples

Download data: CEL

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19580

84 Samples

Download data

(Submitter supplied) Transcriptome profiling (RNA-seq) of local and systemic leaves of Arabidopsis Col treated with 50 mM DPI and exposed to a local treatment of high light

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19580

24 Samples

Download data: TXT

(Submitter supplied) Transcriptome profiling (RNA-seq) of Col seedlings growing in liquid MS culture and exposed to 1 mM H2O2 treatment

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19580

12 Samples

Download data: TXT

(Submitter supplied) Transcriptome profiling (RNA-seq) of local and systemic leaves of Arabidopsis Col and rbohD plants exposed to a local treatment of high light

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19580

48 Samples

Download data: TXT

(Submitter supplied) Salicylic acid (SA) levels increase in Arabidopsis upon exposure to low temperature for more than a week. This cold-induced SA biosynthesis was found to proceed through the isochorismate synthase (ICS) pathway. The sid2-1 mutant is deficient in ICS1 and does not make SA at low temperature. We used microarray analysis to examine the genes differentially regulated by low temperature that were dependent on SA

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

12 Samples

Download data: CEL

(Submitter supplied) CAMTA3 is known to be involved in regulation of induction of early cold-responsive genes, CBF1 and CBF2 at cold conditions and of suppression of SA production at warm temperature. Considering a functional redundancy of CAMTA family members in both warm and cold conditions, high-order of camta mutants, camta double and triple mutants, were generated and used to determined the effects of their regulation using microarray analysis.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platform:

GPL198

21 Samples

Download data: CEL

(Submitter supplied) rs08-03_glutathion - glutathion - How does glutathione content or reduction state affect H2O2-induced changes in the transcriptome? - Three single Arabidopsis mutants were used: cat2, knockout for catalase2 and so enriched in H2O2; cad2, defective in glutathione content; cytGR, knockout for cytosolic glutathione reductase. Cat2 was crossed with cad2 and cyt GR, and col0, 3 single mutants, and 2 double mutants were sampled in controlled growth conditions either in 8h or 16h photoperiod. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Platforms:

GPL6403 GPL9553

40 Samples

Download data: GPR

(Submitter supplied) We have investigated the genomic response of Arabidopsis cell suspension culture under high light. Our main goal has been twofold: first, to establish whether chloroplasts in Arabidopsis cell suspension culture are functional and, as such, can act as sensors of adverse external stimuli leading to the activation of genomic defence responses in a manner similar to that described in whole plants exposed to a wide range of environmental stresses and; second, to distinguish which of the ROS that would be probably generated in the chloroplasts is predominant. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array

Dataset:

GDS3933

Platform:

GPL198

9 Samples

Download data: CEL

Analysis of Arabidopsis cell suspension culture (ACSC), containing functional chloroplasts, subjected to high light. Several reactive oxygen species (ROS) are generated under HL conditions. Results provide insight into molecular mechanisms underlying early defense responses to HL stress.

Organism:

Arabidopsis thaliana

Type:

Expression profiling by array, transformed count, 3 stress sets

Platform:

GPL198

Series:

GSE22671

9 Samples

Download data: CEL

(Submitter supplied) Leaf senescence is a highly coordinated and complicated process with the integration of numerous internal and environmental signals. Salicylic acid (SA) and reactive oxygen species (ROS) are two well-defined inducers of leaf senescence, whose contents progressively and inter-dependently increase during leaf senescence via a yet unknown mechanism. Here, we have characterized a newly identified positive regulator of leaf senescence, WRKY75, and demonstrated that knock-down or knock-out of WRKY75 delays, while over-expression of WRKY75 accelerates age-dependent leaf senescence. more...

Organism:

Arabidopsis thaliana

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13222

2 Samples

Download data: XLS

(Submitter supplied) Background: Cassava is an important tropical root crop adapted to a wide range of environmental stimuli such as drought and acid soils. Nevertheless, it is an extremely cold-sensitive tropical species. Thus far, there is limited information about gene regulation and signaling pathways related to the cold stress response in cassava. The development of microarray technology has accelerated the study of global transcription profiling under certain conditions. more...

Organism:

Manihot esculenta

Type:

Expression profiling by array

Platform:

GPL11271

9 Samples

Download data: TXT