GEO DataSets

(Submitter supplied) 6-Nonadecynoic acid (6-NDA), a plant-derived acetylenic acid, exhibits strong inhibitory activity against the human fungal pathogens Candida albicans, Aspergillus fumigatus, and Trichophyton mentagrophytes. In the present study, transcriptional profiling coupled with mutant and biochemical analyses were conducted using the model organism Saccharomyces cerevisiae to investigate the mechanism of action of this compound. more...

Organism:

Saccharomyces cerevisiae; Candida albicans; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platforms:

GPL15195 GPL2529

14 Samples

Download data: CEL, CHP, TXT

(Submitter supplied) Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with the analysis of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low oxygen conditions. more...

Organism:

Saccharomyces cerevisiae; Candida albicans

Type:

Expression profiling by array

Platforms:

GPL6346 GPL90

9 Samples

Download data: CEL, TXT

(Submitter supplied) Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with the analysis of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low oxygen conditions. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL6346

3 Samples

Download data: TXT

(Submitter supplied) Sampangine, a plant-derived alkaloid found in the Annonaceae family, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans and Aspergillus fumigatus. In the present study, transcriptional profiling experiments coupled with the analysis of mutants were performed in an effort to elucidate its mechanism of action. Using Saccharomyces cerevisiae as a model organism, we show that sampangine produces a transcriptional response indicative of hypoxia, altering the expression of genes known to respond to low oxygen conditions. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS3137

Platform:

GPL90

6 Samples

Download data: CEL

(Submitter supplied) The C. albicans Genome Oligo Set consisting of 70mer probes representing 6266 C. albicans genes was obtained from Qiagen (Valencia, CA), and printed on glass slides by Microarrays, Inc (Nashville, TN).

Organism:

Candida albicans

2 Series

3 Samples

Download data

Analysis of Saccharomyces cerevisiae cells treated with the antifungal compound sampangine. Sampangine exhibits strong inhibitory activity against opportunistic fungal pathogens such as Candida albicans. Using S. cerevisiae as a model, results provide insight into sampangine's mechanism of action.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL90

Series:

GSE10073

6 Samples

Download data: CEL

(Submitter supplied) Plakortide F acid (PFA), a marine-derived polyketide endoperoxide, exhibits strong inhibitory activity against the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. In the present study, transcriptional profiling coupled with mutant and biochemical analyses were conducted using the model organism Saccharomyces cerevisiae, to investigate the mechanism of action of this compound. more...

Organism:

Saccharomyces cerevisiae; Saccharomyces cerevisiae S288C; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL2529

6 Samples

Download data: CEL, CHP

(Submitter supplied) Candida albicans is a commensal yeast within the human microbiota with significant medical importance because of its pathogenic potential. The yeast produces biofilms, which are highly resistant to available antifungals. High level of antifungal resistance by C. albicans biofilms has resulted in the need for alternative treatment. Polyunsaturated fatty acids such as arachidonic acid has been reported to increase the susceptibility of C. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22403

12 Samples

Download data: XLSX

(Submitter supplied) In the pathogenic yeast Candida albicans, the zinc cluster transcription factor Upc2p has been shown to regulate expression of ERG11 and other genes involved in ergosterol biosynthesis upon exposure to azole antifungals. ERG11 encodes lanosterol demethylase, the target enzyme of this antifungal class. Over-expression of UPC2 reduces azole susceptibility, whereas its disruption results in hypersusceptibility to azoles and reduced accumulation of exogenous sterols. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platforms:

GPL5723 GPL6808

18 Samples

Download data: CEL, CHP

(Submitter supplied) Candida albicans was subjected to treatment with tomatidine and samples were taken at 2 different time points (1h, 3h). The results show that tomatidine induces genes involved in ergosterol biosynthesis as compared to untreated controls.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

12 Samples

Download data: XLSX

(Submitter supplied) Transcriptional profiling of Candida albicans comparing fluconazole treated cells with fluconazole and osthole treated cells

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL9728

3 Samples

Download data: LSR

(Submitter supplied) A major mechanism of azole resistance in Candida albicans is the over-expression of the genes encoding the ABC transporters Cdr1p and Cdr2p. Constitutive over-expression of these efflux pumps is due to mutations in the gene encoding Tac1p, resulting in hyperactivity of this zinc cluster transcription factor. In order to identify the transcriptional targets of Tac1p, we examined four matched sets of clinical isolates representing the development of CDR1- and CDR2-mediated azole resistance, using gene expression profiling analysis. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL5723

27 Samples

Download data: CEL, CHP

(Submitter supplied) Soybean toxin (SBTX) is an antifungal protein from soybeans with broad growth and filamentation inhibitory activity against many fungi, including human and plant pathogenic species such as Candida albicans, Candida parapsilosis, Aspergillus niger, Penicillium herquei, Cercospora sojina, and Cerospora kikuchii. Understanding the mechanism by which SBTX acts on fungi and yeasts may contribute towards the design of novel antifungal drugs and/or for the development of transgenic plants resistant to pathogens. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL17199

8 Samples

Download data: TXT

(Submitter supplied) Eupolauridine and liriodenine are plant-derived aporphinoid alkaloids that exhibit potent inhibitory activity against the opportunistic fungal pathogens Candida albicans and Cryptococcus neoformans. However, the molecular mechanism of this antifungal activity is unknown. In this study, we show that eupolauridine 9591 (E9591), a synthetic analog of eupolauridine, and liriodenine methiodide (LMT), a methiodide salt of liriodenine, mediate their antifungal activities by disrupting mitochondrial iron-sulfur (Fe-S) cluster synthesis. more...

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL2529

30 Samples

Download data: CEL, CHP

(Submitter supplied) We present microbial Drop-seq or mDrop-seq, a high-throughput scRNA-seq technique that is used on two yeast species, Saccharomyces cerevisiae, a popular model organism and Candida albicans, a common opportunistic pathogen. We benchmarked mDrop-seq for sensitivity and specificity and used it to profile 35,109 S. cerevisiae cells to detect variation in mRNA levels between them. As a proof of concept, we quantified expression differences in heat-shocked S. more...

Organism:

Saccharomyces cerevisiae; Candida albicans

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19756 GPL22403 GPL28878

23 Samples

Download data: TAR, TXT