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Links from GEO DataSets

Items: 20

1.

RNA-Seq of Saccharomyces cerevisiae cells carrying different YRR1 alleles in response to 4-nitroquinoline-N-oxide (4NQO) and to glycerol as the sole carbon source

(Submitter supplied) In this study, we constructed three isogenic strains of S96 yrr1Δ background (its native YRR1 gene was knocked out) carrying three different YRR1 alleles, YRR1_S96, YRR1_YJM789 and YRR1_S96-I775E, respectively. We then conducted RNA deep sequencing (RNA-Seq) on the three strains grown in Yeast Peptone Dextrose medium (YPD), YPD + 4NQO and Yeast Peptone glycerol medium (YPglycerol).
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL17143
18 Samples
Download data: XLSX
Series
Accession:
GSE74642
ID:
200074642
2.

ChIP-Seq for Yrr1 protein on Saccharomyces cerevisiae cells carrying different YRR1 alleles in response to 4-nitroquinoline-N-oxide (4NQO)

(Submitter supplied) In this study, we constructed three isogenic strains of S96 yrr1Δ background (its native YRR1 gene was knocked out) carrying three different YRR1 alleles, YRR1_S96, YRR1_YJM789, YRR1_S96-I775E, respectively. We then conducted chromatin immuno-precipitation followed by high-throughput sequencing (ChIP-Seq) for Yrr1 protein on the three strains grown in Yeast Peptone Dextrose medium (YPD) and YPD + 4NQO.
Organism:
Saccharomyces cerevisiae
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13821
24 Samples
Download data: XLSX
Series
Accession:
GSE74700
ID:
200074700
3.

BY4741 and BY4741(Δyrr1)transcriptional differences under vanillin stress

(Submitter supplied) BY4741(Δyrr1)exhibited better vanillin tolerance to vanillin than that of wildtype strain. To assess transcriptional differences between these two strains. Yrr1p is a transcriptional factors which activated genes related to multidrug resistance.The transcriptome of BY4741 and BY4741(Δyrr1)under vanillin stress or vanillin free conditions were conducted,respectively
Organism:
Saccharomyces cerevisiae BY4741
Type:
Expression profiling by high throughput sequencing
Platform:
GPL22674
8 Samples
Download data: TXT
Series
Accession:
GSE89854
ID:
200089854
4.

Impact of PDR8 allelic variation on yeast transcriptome profile in wine fermentation condition.

(Submitter supplied) An eQTL analysis show that mutations in PDR8 gene in 59A strain versus S288c could trigger expressions variations of QDR2. In order to confirm this result and highlight other gene expression variations associated to PDR8 allelic variation, we performed an allele switch of PDR8 in 59A background (59A PDR8-S288c) and compared the transcriptomic profile of this strain to 59A. The analysis was performed in wine alcoholic fermentation conditions in stationary phase during nitrogen starvation and in alcoholic stress. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platform:
GPL16012
6 Samples
Download data: GPR
Series
Accession:
GSE41738
ID:
200041738
5.

eQTL linkages in wine yeasts to decipher regulatory networks in wine fermentation

(Submitter supplied) We performed here the transcriptomic profile of 44 segregants from a cross between S288c and 59A (a spore of EC1118 strain). The analysis was performed in wine fermentation condition in stationary phase during nitrogen starvation and in alcoholic stress. These data, associated with an individual genotyping by Affymetrix array allow us to highlight genetic variations involved in perturbation of regulatory network and fermentative behavior.
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platform:
GPL16012
56 Samples
Download data: GPR
Series
Accession:
GSE41025
ID:
200041025
6.

Histone H3Lys4 trimethylation in budding yeast S. cerevisiae for wild type and cnc1Djhd2D yeast mutants

(Submitter supplied) In this study, we measured histone H3Lys4 trimethylation in budding yeast S. cerevisiae for wild type and cnc1Djhd2D yeast mutants. These experiments were performed for yeast cultured to mid-logarithmic phase in non-fermentable carbon.
Organism:
Saccharomyces cerevisiae
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13821
16 Samples
Download data: BEDGRAPH
Series
Accession:
GSE93641
ID:
200093641
7.

Time course of glycine addition or withdrawal

(Submitter supplied) +Gly 20, 40, 80: Cells were grown to early log phase (OD600 ~ 0.2) at 30oC in 1 liter of minimal B medium (Cherest, H., and Surdin-Kerjan, Y. (1992) Genetics 130, 51-58) and t=0 time point samples were harvested (250 ml). The remainder of the medium was supplemented with 10 mM glycine incubated at 30oC and samples (250 ml) harvested at 20, 40 and 80 minutes. All cells were harvested by rapid centrifugation at room temperature then flash frozen in liquid nitrogen. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platforms:
GPL1336 GPL1337
20 Samples
Download data
Series
Accession:
GSE1554
ID:
200001554
8.

Steady state +/- Gly

(Submitter supplied) Cells were grown to early log phase (OD600 ~ 0.2) at 30oC in 1 liter of minimal B medium (Cherest, H., and Surdin-Kerjan, Y. (1992) Genetics 130, 51-58) with or without supplementation with 10 mM glycine and harvested. All cells were harvested by rapid centrifugation at room temperature then flash frozen in liquid nitrogen. Arrays compared expression of Gly- cells to the Gly+ controls. Strain BY4741 was the wild type and several deletion strains were also used. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platform:
GPL1336
8 Samples
Download data
Series
Accession:
GSE1553
ID:
200001553
9.

S. cerevisiae mutant with a constitutively activated Ras/cAMP pathway

(Submitter supplied) Comparison of the transcriptomes of Saccharomyces cerevisiae wild type FY23 and a PDE2 deletion mutant DJ28. Keywords = PDE2 Keywords = Ras/cAMP pathway Keywords: other
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platform:
GPL422
10 Samples
Download data
Series
Accession:
GSE600
ID:
200000600
10.

Comparative transcriptome analysis of basal gene expression in Wild-type and Sen1ΔN mutant of Saccharomyces cerevisiae

(Submitter supplied) In Saccharomyces cerevisiae, Sen1 is a 252-kDa, nuclear superfamily-1 RNA/DNA helicase that encoded by an essential gene SEN1 (Senataxin). It is an important component of the Nrd1p-Nab3p-Sen1p (NRD1) complex that regulates the transcriptional termination of most non-coding and some coding transcripts at RNA polymerase pause sites. Sen1 specifically interacts with Rnt1p (RNase III), an endoribonuclease, and with Rpb1p (Rpo21p), a subunit of RNA polymerase II, through its N-terminal domain (NTD), which is a critical element of the RNA-processing machinery. more...
Organism:
Saccharomyces cerevisiae; Schizosaccharomyces pombe
Type:
Expression profiling by array
Platform:
GPL2529
4 Samples
Download data: CEL
Series
Accession:
GSE75447
ID:
200075447
11.

Genetic Analysis of Variation in Transcription Factor Binding in Yeast

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array; Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL9134 GPL9825
222 Samples
Download data: TXT
Series
Accession:
GSE19636
ID:
200019636
12.

Genome-wide Ste12-binding site mapping in MATa segregants of YJM789 x S96 cross

(Submitter supplied) In this study, we mapped for the first time differences in transcription binding among individuals and elucidated the genetic basis of such variation. Whole-genome Ste12 binding profiles were determined using ChIP-Seq in pheromone-treated cells of 43 segregants of a cross between two highly diverged yeast strains, YJM789 and S288c as well as the parental lines. We identified extensive Ste12 binding variation among individuals and mapped underlying cis- and trans- acting loci responsible for such variation. more...
Organism:
Saccharomyces cerevisiae
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL9134
174 Samples
Download data: TXT
Series
Accession:
GSE19635
ID:
200019635
13.

Gene Expression of MATa yeast segregants (YJM789 X S96) after alpha factor treatment

(Submitter supplied) In this study, we mapped for the first time differences in transcription binding among individuals and elucidated the genetic basis of such variation. Whole-genome Ste12 binding profiles were determined using ChIP-Seq in pheromone-treated cells of 43 segregants of a cross between two highly diverged yeast strains, YJM789 and S288c, as well as the parental lines. We identified extensive Ste12 binding variation among individuals and mapped underlying cis- and trans- acting loci responsible for such variation. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platform:
GPL9825
48 Samples
Download data: TXT
Series
Accession:
GSE19634
ID:
200019634
14.

mRNA expression data in Δrsf1mutant during growth on, and transition to growth on glycerol as sole carbon source

(Submitter supplied) Rsf1p is a putative transcription factor required for efficient growth using glycerol as sole carbon source but not for growth on the alternative respiratory carbon source ethanol. We use microarrays to determine the differences in the transcriptional program between the Δrsf1 mutant and the wild type during respiratory growth on glycerol as well as the transition to growth on glycerol as sole carbon source. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platform:
GPL90
22 Samples
Download data: CEL, CHP
Series
Accession:
GSE10031
ID:
200010031
15.

Functional Genomics Analysis of the Yeast Iron Responsive Transcription Factor Aft1 Reveals Iron-Independent Functions

(Submitter supplied) The Saccharomyces cerevisiae transcription factor Aft1 is activated in iron-deficient cells to induce the expression of iron regulon genes, which coordinate the increase of iron uptake and remodel cellular metabolism to survive low iron conditions. In addition, Aft1 has been implicated in numerous cellular processes including cell cycle progression and chromosome stability; however it is unclear if all cellular effects of Aft1 are mediated through iron homeostasis. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by array
Platform:
GPL10104
12 Samples
Download data: GPR
Series
Accession:
GSE20531
ID:
200020531
16.

Transcriptional profiling of staurosporine-induced cell death in wild type versus Δczt-1 in Neurospora crassa

(Submitter supplied) Purpose: Compare the transcriptional profile of staurosporine-treated cells in Neurospora crassa wild type and Δczt-1 (ΔNCU09974) cells Methods: Conidial suspensions were obtained and 1 x 106 cells/ml incubated in minimal medium for 6 hours (26ºC, 140 rpm, constant light) followed by the addition of staurosporine (or DMSO) and growth for 1 more hour. Cells were harvested using 0.45 μm filters and immediately frozen in liquid nitrogen. more...
Organism:
Neurospora crassa
Type:
Expression profiling by high throughput sequencing
Platform:
GPL16164
4 Samples
Download data: TXT
Series
Accession:
GSE52153
ID:
200052153
17.

The yeast Snt2 protein helps coordinate the transcriptional response to hydrogen-peroxide mediated oxidative stress

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13821
66 Samples
Download data: WIG
Series
Accession:
GSE43002
ID:
200043002
18.

The yeast Snt2 protein helps coordinate the transcriptional response to hydrogen-peroxide mediated oxidative stress (rapamycin or DMSO)

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...
Organism:
Saccharomyces cerevisiae
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13821
30 Samples
Download data: WIG
Series
Accession:
GSE43001
ID:
200043001
19.

The yeast Snt2 protein helps coordinate the transcriptional response to hydrogen-peroxide mediated oxidative stress (RNA-seq)

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...
Organism:
Saccharomyces cerevisiae
Type:
Expression profiling by high throughput sequencing
Platform:
GPL13821
18 Samples
Download data: WIG
Series
Accession:
GSE42983
ID:
200042983
20.

The yeast Snt2 protein helps coordinate the transcriptional response to hydrogen-peroxide mediated oxidative stress (H2O2)

(Submitter supplied) Snt2 is a yeast chromatin-interacting protein whose function has not been well characterized, that was recently shown to associate with Ecm5 and the Rpd3 deacetylase. Using chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq), we show that in response to H2O2, Snt2 and Ecm5 colocalize to promoters of genes involved in various aspects of the environmental stress response. By integrating these ChIP-seq results with expression analysis, we identify a key set of target genes that require Snt2 for proper expression after H2O2 stress. more...
Organism:
Saccharomyces cerevisiae
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13821
18 Samples
Download data: WIG
Series
Accession:
GSE42971
ID:
200042971
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