GEO DataSets

(Submitter supplied) Investigation of whole genome expression level changes in Bacillus anthracis Sterne deltaClpX mutant compared to the wild-type strain after growth in nutrient rich media. The deltaClpX mutant used in this study is described in McGillivray et al. 2009. ClpX Protease Contributes to Antimicrobial Peptide Resistance and Virulence Phenotypes of Bacillus anthracis. Journal of Innate Immunity 1(5): 494-506.

Organism:

Bacillus anthracis str. Sterne

Type:

Expression profiling by array

Platform:

GPL23020

6 Samples

Download data: TXT, XLSX

(Submitter supplied) We assesed the transcriptional response of wild-type and isogenic lrgAB mutant to growth in the presence of three different stress conditions (aerobiosis, high-temperature, and the cell-wall active antibiotic vancomycin) by using RNA-seq

Organism:

Streptococcus mutans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL21130

24 Samples

Download data: TXT

(Submitter supplied) Bacillus anthracis is a spore-forming bacterium that causes devastating infections and has been used as a bioterror agent. This pathogen can survive hostile environments through the signaling activity of two-component systems, which couple environmental sensing with transcriptional activation to initiate a coordinated response to stress. In this work, we describe the identification of a two-component system, EdsRS, which mediates the B. more...

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27932

12 Samples

Download data: XLSX

(Submitter supplied) To investigate the response of S. pneumoniae to three distinct antimicrobial peptides (AMPs), i.e. bacitracin, nisin and LL-37, transcriptome analysis of challenged bacteria was performed. Only a limited number of genes was found to be up- or down-regulated in all cases. Several of these common highly induced genes were chosen for further analysis, i.e. SP0385-0387, SP0912-0913, SP0785-0787, SP1714-1715 and the blp cluster. more...

Organism:

Streptococcus pneumoniae; Streptococcus pneumoniae D39

Type:

Expression profiling by array

Platform:

GPL9459

6 Samples

Download data: TXT

(Submitter supplied) It is very important to understand the mechanisms how bacteria become tolerant towards antibiotics during clinical therapy. In a previous study we showed that increased daptomycin (DAP) tolerance of Staphylococcus aureus was due to a point mutation in pitA (inorganic phosphate transporter) that led to intracellular accumulation of both inorganic phosphate (Pi) and polyphosphate (polyP). DAP tolerance in that pitA6 mutant differs from classical resistance mechanisms as there was no increase in minimal inhibitory concentration (MIC). more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL10537

8 Samples

Download data: TXT

(Submitter supplied) Abstract of associated manuscript: Daptomycin is the first of a new class of cyclic lipopeptide antibiotics used against multidrug-resistant Gram-positive pathogens. The proposed mechanism of action involves disruption of the functional integrity of the bacterial membrane in a Ca2+-dependent manner. We have used transcriptional profiling to demonstrate that treatment of Bacillus subtilis with daptomycin strongly induces the lia operon including the autoregulatory LiaRS two-component system (homologous to Staphylococcus aureus VraSR). more...

Organism:

Bacillus subtilis

Type:

Expression profiling by array

Platform:

GPL7420

3 Samples

Download data: GPR

(Submitter supplied) Daptomycin is an extensively used anti-staphylococcal agent due to the rise in methicillin-resistant Staphylococcus aureus. However, both laboratory-derived and clinical decreased susceptibility isolates have been described, but the mechanism(s) of resistance is poorly understood. To further understand daptomycin resistance, comparative genome sequencing, transcriptomics, ultrastructure ,and cell envelope studies were carried out on two relatively higher level (4 and 8 ug/ml-1) laboratory-derived daptomycin-resistant strains (strains CB1541 and CB1540 respectively) compared to their methicillin-resistant parent strain (CB1118;MW2). more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5881

6 Samples

Download data: TXT

(Submitter supplied) S. aureus possesses 16 two-component systems (TCS), two of which (GraRS & NsaRS) belong to the intramembrane-sensing histidine kinase (IM-HK) family, conserved within the firmicutes. NsaRS has recently been documented as being important for nisin-resistance in S. aureus. In this study we present a detailed characterization of NsaRS and reveal that, as with other IM-HK TCS, it responds to disruptions in cell-wall stability. more...

Organism:

Staphylococcus aureus

Type:

Expression profiling by array

Platform:

GPL5879

4 Samples

Download data: GPR

(Submitter supplied) Spx, a member of the ArsC (arsenate reductase) protein family, is highly conserved in Gram-positive bacteria, where it interacts with RNA polymerase to activate transcription in response to toxic oxidants.  In Bacillus anthracis, resistance to oxidative stress requires the activity of two paralogous forms of Spx, SpxA1 and SpxA2.   Suppressor mutations were identified in spxA1 mutant cells that conferred resistance to hydrogen peroxide. more...

Organism:

Bacillus anthracis

Type:

Expression profiling by array

Platform:

GPL10189

6 Samples

Download data: MEV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL31186

20 Samples

Download data

(Submitter supplied) Formaldehyde cross-linking RNA immunoprecipitation coupled with illumina sequencing (fRIP-seq) was performed to pinpoint the direct RNA targets of KrrA using a FLAG-tagged KrrA construct that is driven by a constitutive promoter Plgt (pOS1.PlgtkrrA-FLAG).

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL31186

8 Samples

Download data: XLSX

(Submitter supplied) To uncover the effects of KrrA regulation on gene transcription and define the bacterial response imposed by this regulation, a transcriptomic study was carried out in which Bacillus anthracis krrA was compared to WT in two growth conditions: LB in the presence or absence of ‘205.

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platform:

GPL31186

12 Samples

Download data: XLSX

(Submitter supplied) We then performed gene expression profiling using data obtained from RNA-seq of 2 different cells at three time points.

Organism:

Bacillus thuringiensis serovar kurstaki str. HD73

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32767

18 Samples

Download data: TAB

(Submitter supplied) To determine Sigma 54 (SigL) reglons in Bacillus thuringiensis HD73 strain, A sigLmutant, HD(ΔsigL::kan), was constructed with insertion of kanamycin resistance gene cassete. We have employed whole genome microarray expression profiling as a discovery platform to identify the difference of gene expression between mutant and wild-type strains.

Organism:

Bacillus thuringiensis

Type:

Expression profiling by array

Platform:

GPL17384

6 Samples

Download data: TXT

(Submitter supplied) Although gene expression has been studied in bacteria for decades, many aspects of the bacterial transcriptome remain poorly understood. Transcript structure, operon linkages, and absolute abundance information all provide valuable insights into gene function and regulation, but none has ever been determined on a genome-wide scale for any bacterium. Here we report the first comprehensive and unbiased definition of a bacterial transcriptome under various growth conditions. more...

Organism:

Bacillus anthracis

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL9410 GPL9411

8 Samples

Download data: QUAL, TXT

(Submitter supplied) The High Temperature Requirement (HtrA) chaperone/protease is involved in the stress-response of the anthrax-causing pathogen Bacillus anthracis. Resilience to oxidative stress is essential for manifestation of B. anthracis pathogenicity. We submitt transcriptome data sets detailing global gene expression in B. anthracis wild-type and htrA-disrupted strains following H2O2-induced oxidative stress.

Organism:

Bacillus anthracis str. Vollum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL28578

14 Samples

Download data: TXT