GEO DataSets

(Submitter supplied) The cell wall is essential for viability of fungi and is an effective drug target in pathogens such as Candida albicans. The contribution of posttranscriptional gene regulators to cell wall integrity in C. albicans is unknown. We show that the C. albicans Ccr4-Pop2 mRNA deadenylase, a regulator of mRNA stability and translation, is required for cell wall integrity. The ccr4/pop2 mutants display reduced wall β-glucans and sensitivity to the echinocandin caspofungin. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL9818

4 Samples

Download data: TXT

(Submitter supplied) The Poly(A)-Tail focused RNA-seq, or PAT-seq approach, is an affordable and efficient tool for the measure of 3’UTR dynamics. We show here that PAT-seq returns (i) digital gene expression, (ii) polyadenylation site usage within and between samples, including alternative adenylation, and (iii) the polyadenylation-state the transcriptome. PAT-seq differs from previous 3’ focused RNA-seq methods in that it strictly depends on native 3’ adenylation within total RNA samples and thus removes the need for ribosome depletion and, that the full native poly(A)-tail is included in the sequencing libraries. more...

Organism:

Saccharomyces cerevisiae

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL18085

13 Samples

Download data: CSV

(Submitter supplied) This set of experiment was done in order to analyze the effect of a dysfunctional mitochondria on C. albicans. The mutant was obtained by deleting the gene FZO1, which is known to be involved in mitochondrial biogenesis in S. cerevisiae. We show that the deletion of FZO1 leads to a change in mitochondrial morphology, which affects the mitochondrial membrane potential and causes the loss of mtDNA. Upon performing a transcriptome analysis, we observed that the mutant showed upregulation of genes like AOX2, MDR1 etc., while the genes involved in iron uptake were dysregulated. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL13440

6 Samples

Download data: TXT

(Submitter supplied) The emergence of drug-resistant fungal strains is a threat to human health. Identifying targets that increase the susceptibility of fungal pathogens to current therapies will significantly improve outcomes. Tra1 is an essential component of the SAGA and NuA4 transcriptional co-activator complexes and is linked to multiple cellular processes associated with the yeast response to antifungal drugs. This submission contains RNA sequencing data from Candida albicans strain expressing a Tra1 mutant with three arginine to glutamine mutations in the PI3K domain (tra1Q3; Berg et al., 2018) treated with or without the antifungal caspofungin. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15645

12 Samples

Download data: TXT

(Submitter supplied) Tra1 is an essential component of the SAGA and NuA4 transcriptional co-activator complexes and is linked to multiple cellular processes associated with the yeast response to antifungal drugs. This submission contains RNA sequencing data from Saccharomyces cerevisiae strains expressing a Tra1 mutant with three arginine to glutamine mutations in the PI3K domain (tra1Q3; Berg et al., 2018). Our goal was to identify how gene expression changes due to mutation to the PI3K domain of Tra1.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13821

6 Samples

Download data: TXT

(Submitter supplied) We report the expression levels of transcripts of 5,901 genes in log-phase growing cells of the wild type CAI4 as well as the gdt1/gdt1, the pmr1/pmr1, and the gdt1/gdt1 pmr1/pmr1 mutants.We find that as compared to the wild type, there are 249, 446 and 440 differentially expressed genes (DEGs) in the gdt1/gdt1, the pmr1/pmr1 and the gdt1/gdt1 pmr1/pmr1 mutants, respectively. Deletion of CaGDT1 does not affect the transcript level of CaPMR1, and vice versa. more...

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

4 Samples

Download data: XLS

(Submitter supplied) The cell wall-targeting echinocandin antifungals, although potent and well-tolerated, are inadequate in treating fungal infections due to their narrow spectrum of activity and their propensity to induce pathogen resistance. A promising strategy to overcome these drawbacks is to combine echinocandins with a molecule that improves their activity and also disrupts drug adaptation pathways. In this study, we show that puupehenone (PUUP), a marine sponge-derived sesquiterpene quinone potentiates the echinocandin drug, caspofungin (CAS) in CAS-resistant fungal pathogens. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19756

12 Samples

Download data: XLS

(Submitter supplied) Goal: Phenotypic evidence suggested that the transcription factor Czf1 influences the integrity of the cell wall. RNA-seq was performed to identify cell-wall related genes whose expression changes when Czf1 is artificially activated. Result: The set of genes whose expression is increased at least 4-fold is significantly enriched for genes whose GO (gene ontology) terms are "cell surface" and "cell wall", as well as several known and putative mannosyltransferases.

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19036

9 Samples

Download data: TXT, XLSX

(Submitter supplied) To determine potential roles of three transcript factors (Rbf1p, Hfl1p and Dpb4p) in cell metabolic activities and other cellular bioprocesses, we have performed genomic microarray in each gene knockout strain and transcription profiles were compared to its parental strain SN250

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL18166

4 Samples

Download data: TXT

(Submitter supplied) The S. cerevisiae Pop2 protein is an exonuclease in the Ccr4-Not complex that is a conserved regulator of gene expression. Pop2 regulates gene expression post-transcriptionally by shortening the poly(A) tail of mRNA. A previous study has shown that Pop2 is phosphorylated at threonine 97 (T97) by Yak1 protein kinase in response to glucose limitation. However, the physiological importance of Pop2 phosphorylation remains unknown. more...

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

8 Samples

Download data: CEL

(Submitter supplied) We examined the early gene expression profiles (30 min) of log-phase wild-type C. albicans growing in YPD at 30 °C in response to respiration inhbitors 1mM SNP, 0.5 mM SHAM, or 1 mM SNP+ 0.5 mM SHAM combination

Organism:

Candida albicans

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22403

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Candida albicans

Type:

Expression profiling by array; Genome binding/occupancy profiling by array

Platforms:

GPL13696 GPL13813

32 Samples

Download data: GPR

(Submitter supplied) The mammalian gastrointestinal tract and the bloodstream are highly disparate biological niches, and yet certain commensal-pathogenic microorganisms are able to thrive in both environments. Here, we report the evolution of a unique transcription circuit in the yeast, Candida albicans, which determines its fitness in both host niches. Our comprehensive analysis of the DNA-binding proteins that regulate iron uptake by this organism suggests the evolutionary intercalation of a transcriptional activator called Sef1 between two broadly conserved transcriptional repressors, Sfu1 and Hap43. more...

Organism:

Candida albicans

Type:

Genome binding/occupancy profiling by array

Platform:

GPL13696

10 Samples

Download data: GPR

(Submitter supplied) The mammalian gastrointestinal tract and the bloodstream are highly disparate biological niches, and yet certain commensal-pathogenic microorganisms are able to thrive in both environments. Here, we report the evolution of a unique transcription circuit in the yeast, Candida albicans, which determines its fitness in both host niches. Our comprehensive analysis of the DNA-binding proteins that regulate iron uptake by this organism suggests the evolutionary intercalation of a transcriptional activator called Sef1 between two broadly conserved transcriptional repressors, Sfu1 and Hap43. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL13813

22 Samples

Download data: GPR

(Submitter supplied) Two-component signal transduction pathways are one of the primary means by which microorganisms respond to environmental signals. These signaling cascades originated in prokaryotes and were inherited by eukaryotes via endosymbiotic lateral gene transfer from ancestral cyanobacteria. We report here that the nuclear genome of pathogenic fungus Candida albicans contains elements of a two-component signaling pathway that seem to be targeted to the mitochondria. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL6808

8 Samples

Download data: CEL

(Submitter supplied) To estiamte the role lipid flippase subunit Cdc50 plays in Candida glabrata, a Δcdc50 null mutant was constructed based on standard strain ATCC2001 (wild type, WT) through homologous recombination. Then, gene expression profiling analysis was performed using data obtained from RNA-seq of WT and Δcdc50 mutant.

Organism:

Nakaseomyces glabratus CBS 138

Type:

Expression profiling by high throughput sequencing

Platform:

GPL32820

6 Samples

Download data: TXT

(Submitter supplied) The fungal pathogen Candida albicans produces dark-pigmented melanin when grown in a basal medium containing 1 mM l-DOPA as melanin substrate. In the widely used C. albicans strain SC5314, melanin appeared after 3-4 days of incubation in l-DOPA medium. The experiment was designed to reveal cadidate genes associated with melanin biosynthesis by expression profiling at different times of growth with and without L-DOPA added to the medium. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL7476

16 Samples

Download data: TXT

(Submitter supplied) The cyclic AMP-protein kinase A pathway has a central role in the biology of Candida albicans, a prominent fungal pathogen of humans. The two catalytic subunits for cyclic AMP-dependent protein kinase, Tpk1 (orf19.4892) and Tpk2 (orf19.2277), have divergent roles, and most studies indicate a more pronounced role for Tpk2. Here we dissect two Tpk1-responsive properties: adherence and cell wall integrity. more...

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL9818

16 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL10903

21 Samples

Download data: GPR

(Submitter supplied) Whole genome microarray were used to generate the transcriptional profile of C.albicans UPC2 delete in hypoxia.

Organism:

Candida albicans

Type:

Expression profiling by array

Platform:

GPL10903

9 Samples

Download data: GPR