GEO DataSets

(Submitter supplied) A manila clam oligo microarray platform (GPL10900) was used to profile gene expression in digestive gland of R. philippinarum sampled in four seasons in 4 different areas of Venice Lagoon. For each tissue, total RNA was extracted from four (4) independent biological replicates of digestive gland, each consisting of tissue pools of five (5) animals.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

64 Samples

Download data: TXT

(Submitter supplied) Digestive Gland Samples: A manila clam oligo microarray platform (GPL10900) was used to profile gene expression in digestive gland of R. philippinarum. Total RNA was extracted from three (3) independent biological replicates of digestive gland for each sampling site, each consisting of tissue pools of five (5) animals. Statistical analysis with SAM (Significance Analysis of Microarray) identified1,127 probes differentially expressed. more...

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

12 Samples

Download data: TXT

(Submitter supplied) A manila clam oligo microarray platform (GPL10900) was used to profile gene expression in gills and digestive gland of R. philippinarum. For each tissue, total RNA was extracted from three (3) independent biological replicates of digestive gland and gills, each consisting of tissue pools of five (5) animals. Statistical analysis with SAM (Significance Analysis of Microarray) identified 8,257 probes differentially expressed between the two different tissues.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

6 Samples

Download data: TXT

(Submitter supplied) Four pools of digestive glands were treated as biological replicates in order to evaluate the repeatability of Ruditapes philippinarum oligo microarray platform.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

4 Samples

Download data: TXT

(Submitter supplied) An R.decussatus microarray platform was developed to to profile gene expression in R. decussatus heavy infected by Perkinsus olseni

Organism:

Ruditapes decussatus

Type:

Expression profiling by array

Platform:

GPL15303

8 Samples

Download data: TXT

(Submitter supplied) A Ruditapes philippinarum microarray platform was developed to assess variations on transcritpomic response to copper exposures in Manila clam colelctted in Venice lagoon areas subjected to different anthropogenic impact

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL20320

68 Samples

Download data: TXT

(Submitter supplied) The Manila clam (Ruditapes philippinarum) is a cultured bivalve species with high worldwide commercial importance. Nevertheless, diseases can cause high economical losses. For this reason, the study of immune genes in bivalve mollusks has increased in the last years. The present work describes the construction of the first R. philippinarum microarray containing immune-related hemocyte sequences and its application for the study of the gene transcription profiles of hemocytes from clams challenged with Vibrio alginolyticus through a time course.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL16450

36 Samples

Download data: TXT

(Submitter supplied) A sea bass oligo microarray platform was used to profile gene expression in whole heads of 38 days-old sea bass affected by prognathism, a skeletal malformation that strongly affects sea bass production. Two different conditions: i) prognathous individuals, and ii) normal individuals were analyzed. For each condition, total RNA was extracted from three (3) independent biological replicates, each consisting of pools of five (5) heads. more...

Organism:

Dicentrarchus labrax

Type:

Expression profiling by array

Platform:

GPL9663

6 Samples

Download data: TXT

(Submitter supplied) A sea bass oligo microarray platform was used to profile gene expression in mandibles of 58 days-old sea bass affected by prognathism, a skeletal malformation that strongly affects sea bass production. Two different conditions: i) protruding jaws, and ii) normal jaws were used for gene expression analysis. For each condition, total RNA was extracted from four (4) independent biological replicates, each consisting of pools of five (5) jaws. more...

Organism:

Dicentrarchus labrax

Type:

Expression profiling by array

Platform:

GPL9663

8 Samples

Download data: TXT

(Submitter supplied) The Manila clam (Ruditapes philippinarum) is the bivalve species with the highest world production from both fisheries and aquaculture, but its production is seriously threatened by perkinsosis, a disease caused by the protozoan parasite Perkinsus olseni. To understand the molecular mechanisms underlying R. philippinarum–P. olseni interaction, we analyzed the gene expression profiles of in vitro challenged clam hemocytes and P. more...

Organism:

Perkinsus olseni; Ruditapes philippinarum

Type:

Expression profiling by array

Platforms:

GPL23501 GPL27953

48 Samples

Download data: TXT

(Submitter supplied) The Manila clam (Ruditapes philippinarum) is the bivalve species with the highest world production from both fisheries and aquaculture, but its production is seriously threatened by perkinsosis, a disease caused by the protozoan parasite Perkinsus olseni. To understand the molecular mechanisms underlying R. philippinarum–P. olseni interaction, we analyzed the gene expression profiles of in vitro challenged clam hemocytes and P. more...

Organism:

Perkinsus olseni

Type:

Expression profiling by array

Platform:

GPL27953

16 Samples

Download data: TXT

(Submitter supplied) The Manila clam (Ruditapes philippinarum) is the bivalve species with the highest world production from both fisheries and aquaculture, but its production is seriously threatened by perkinsosis, a disease caused by the protozoan parasite Perkinsus olseni. To understand the molecular mechanisms underlying R. philippinarum–P. olseni interaction, we analyzed the gene expression profiles of in vitro challenged clam hemocytes and P. more...

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL23501

32 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of different clam tissues (hemolymph, extrapallial fluid and mantle) in response to brown ring disease; Brown ring disease (BRD) is a bacterial infection affecting the economically-important clam Ruditapes philippinarum. The disease is caused by a bacterium, Vibrio tapetis, that colonizes the edge of the mantle, altering the biomineralization process and normal shell growth. more...

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL18750

36 Samples

Download data: GPR

(Submitter supplied) The production of Manila clam (Ruditapes philippinarum) is seriously threatened by the protistan parasite Perkinsus olseni. We characterized and compared gene expression of Manila clam haemocytes in response to P. olseni in a time-course (10 h, 24 h, 8 d) controlled laboratory challenge (LC), representing the first steps of infection, and in a more complex infection in the wild (WI) using a validated oligo-microarray containing 11,232 mostly annotated transcripts. more...

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL23501

16 Samples

Download data: TXT

(Submitter supplied) A microarray platform for Solea solea was developed to study gene expression profiles of common sole developmental stages.

Organism:

Solea solea

Type:

Expression profiling by array

Platform:

GPL16124

31 Samples

Download data: TXT

(Submitter supplied) Parasites of the genus Perkinsus spp. cause high mortalities and economic losses to the most noticeable bivalves produced in the worldwide aquaculture. In this study, we analyze how P. olseni influences the gene expression profiles of hemocytes from Manila clam (Venerupis philippinarum) using experimental infections along a temporal series and a Manila clam immune-enriched DNA microarray.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL16450

24 Samples

Download data: TXT

(Submitter supplied) We examined gene expression profiling of native mussels that were sampled in early summer 2003 from sites of the Venice lagoon area known to be differently affected by chemical pollution: Sites 1 and 2 close to the industrial district of Marghera and Site 3 close to the Lido lagoon outlet. Site 4, a current mussel farm located offshore, has been chosen as source of reference targets for microarray hybridizations. more...

Organism:

Mytilus galloprovincialis

Type:

Expression profiling by array

Platform:

GPL1799

6 Samples

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(Submitter supplied) We examined gene expression profiling of mussels treated with two chemical mixtures composed by heavy metals or organic compounds, both including genotoxic agents (cadmium, benzo[a]pyrene, 1-nitro/amino-pyrene) and other contaminants (cupper, mercury, fluoranthene, TCDD). For digestive gland one RNA sample from control mussels and one from each treatment group (7 mussels) were competitively hybridized on the MytArray 1.0. more...

Organism:

Mytilus galloprovincialis

Type:

Expression profiling by array

Platform:

GPL1799

4 Samples

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(Submitter supplied) We examined tissue specificity of gene expression in control mussels. Total RNA was isolated from gills (G), digestive gland (DG), tissues involved in contraction/motility (FML: foot/adductor muscles/ligaments) and reproduction (GM: gonads and mantle) producing four tissue-specific samples. Total RNA was reverse-transcribed and labeled using a Cy3-dCTP and Cy5-dCTP direct incorporation. Each sample was hybridized in competition with the total pool (equal RNA amounts from G, DG, GM and FML) and the relative abundance of each gene was measured by directly comparing fluorescent signals for each probe. more...

Organism:

Mytilus galloprovincialis

Type:

Expression profiling by array

Platform:

GPL1799

8 Samples

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(Submitter supplied) MytArray 1.0 is a cDNA platform, that consists of 1,712 different clones obtained from systematic sequencing of multiple Mytilus galloprovincialis tissues cDNA library and 46 unrelated controls (ESTs from Coffea and bacterial species) printed in duplicate and twice per slide. This library was produced using a strategy that allows the selection of a short 3’-end region of mRNAs and the tagging of each transcript with an unique probe. more...

Organism:

Mytilus galloprovincialis

6 Series

68 Samples

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