GEO DataSets

(Submitter supplied) Furan is a mouse and rat hepatocarcinogen. We sought to determine the dose-dependent changes in gene expression upon exposure of B6C3F1 mice to furan in order to better understand furan’s mode of action. Adult female B6C3F1 mice were exposed to 1, 2, 4 or 8 mg/kg bw furan or vehicle control (corn oil) for three weeks and sacrificed four hours after the final exposure.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

23 Samples

Download data: TXT

(Submitter supplied) Furan is a mouse and rat hepatocarcinogen. We sought to determine the dose-dependent changes in gene expression upon exposure of B6C3F1 mice to furan in order to better understand furan’s mode of action.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

14 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Rattus norvegicus

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL15084 GPL14860

65 Samples

Download data: TXT

(Submitter supplied) Furan is a widely used industrial chemical and a common contaminant in heated foods. Chronic furan exposure has been shown to cause cholangiocarcinoma and hepatocellular tumors at doses of 2 mg/kg bw/day with gender differences in frequency and severity. The hepatic transcriptional alterations induced by low doses of furan (doses below those inducing liver tumors) and the potential mechanisms underlying gender differences are largely unexplored. more...

Organism:

Rattus norvegicus

Type:

Non-coding RNA profiling by array

Platform:

GPL14860

16 Samples

Download data: TXT

(Submitter supplied) Furan is a widely used industrial chemical and a common contaminant in heated foods. Chronic furan exposure has been shown to cause cholangiocarcinoma and hepatocellular tumors at doses of 2 mg/kg bw/day with gender differences in frequency and severity. The hepatic transcriptional alterations induced by low doses of furan (doses below those inducing liver tumors) and the potential mechanisms underlying gender differences are largely unexplored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL15084

49 Samples

Download data: TXT

(Submitter supplied) Acute effects caused by the non-genotoxic carcinogen and peroxisome proliferator (PP) diethylhexylphthalate (DEHP) in the mouse liver

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

24 Samples

Download data: CEL

(Submitter supplied) In this study, we measured liver miRNA in male B6C3F1 mice exposed to a known chemical activator of the peroxisome proliferator-activated receptor alpha (PPARα) pathway, di(2-ethylhexyl) phthalate (DEHP), for 7 and 28 days at concentrations of 0, 750, 1500, 3000, or 6000 ppm in feed. At the highest dose tested, DEHP altered 61 miRNAs after 7 days and 171 miRNAs after 28 days of exposure, with 48 overlapping miRNAs between timepoints. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL19057

48 Samples

Download data: TXT

(Submitter supplied) Acetamide (CAS 60-35-5) is detected in common foods including milk, eggs, beef, and roasted coffee beans. Chronic rodent bioassays using high doses (≥1000 mg/kg/day) suggest acetamide is a group 2B possible human carcinogen due to the induction of liver tumors. Weight-of-evidence indicates acetamide is not genotoxic, and therefore a threshold response is expected. We used a toxicogenomics approach in Wistar rats gavaged daily for 7 and 28 days to determine the benchmark dose (BMD), and investigate its mode of action from differential gene expression. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20084

145 Samples

Download data: TXT

(Submitter supplied) The current test strategy for carcinogenicity is generally based on in vitro and in vivo genotoxicity assays. Non-genotoxic carcinogens (NGTXC) are negative for genotoxicity and go undetected. Therefore, alternative tests to detect these chemicals are urgently needed. NGTXC act through different modes of action, which complicates the development of such assays. We have demon­strated recently in primary mouse hepatocytes that some, but certainly not all, NGTXC can be categorized according to their mode of action based on feature detection at a gene expression level (Schaap et al. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL17198

88 Samples

Download data: CEL

(Submitter supplied) The current test strategy for carcinogenicity is generally based on in vitro and in vivo genotoxicity assays. Non-genotoxic carcinogens (NGTXC) are negative for genotoxicity and go undetected. Therefore, alternative tests to detect these chemicals are urgently needed. NGTXC act through different modes of action, which complicates the development of such assays. We have demonstrated recently in primary mouse hepatocytes that some, but certainly not all, NGTXC can be categorized according to their mode of action based on feature detection at a gene expression level (Schaap et al. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL15125

111 Samples

Download data: CEL, TXT

(Submitter supplied) Many innovative techniques and scientific improvements are available to tackle societal concerns, like public health safety and confining the risk of cancerous exposure to chemicals, but have not been thoroughly tested and implicated yet. We investigated if microRNA and mRNA transcription profiles can be implemented in a short-term carcinogen classifier assay. Our study is additionally focusing on the drawbacks of present-day carcinogen screening strategies and also aims to contribute to a more ethical approach towards animal use and welfare within risk assessment. more...

Organism:

Homo sapiens; Mus musculus; Rattus norvegicus

Type:

Non-coding RNA profiling by array

Platform:

GPL16560

68 Samples

Download data: TXT

(Submitter supplied) In this study, we evaluated the utility of microRNA (miRNA) profiling in female B6C3F1 mouse liver to indicate mechanisms of liver perturbation due to short-term exposure of the known rodent liver hepatotoxicant and carcinogen, furan. Analyses indicated a robust dose response for 34 miRNAs to furan and involvement of p53-linked pathways in furan-mediated hepatotoxicity. Overall, these results indicate mechanistic involvement of miRNA in furan carcinogenicity and provide evidence of their potential utility as accessible biomarkers of exposure and disease.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by high throughput sequencing

Platform:

GPL16173

24 Samples

Download data: TXT

(Submitter supplied) Dibenzo[def,p]chrysene (DBC) is the most carcinogenic polycyclic aromatic hydrocarbon (PAH) examined to date. We investigated the immunotoxicity of DBC, manifested as spleen atrophy following acute exposure of adult Muta™Mouse males by oral gavage. Mice were exposed to 0, 2.0, 6.2, or 20.0 mg DBC /kg-bw per day, for three days. Genotoxic endpoints (DBC-DNA adducts and lacZ mutant frequency in spleen and bone marrow, and red blood cell micronucleus frequency) and global gene expression changes were measured. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

37 Samples

Download data: TXT

(Submitter supplied) In order to screen candidate genes that discriminate genotoxic hepatocarcinogens from nongenotoxic ones, we compared gene expression in the liver of mice treated with 6 genotoxic hepatocarcinogens or 5 nongenotoxic hepatocarcinogens.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL339

52 Samples

Download data: CEL, CHP

(Submitter supplied) Furan is a heterocyclic organic compound produced in the chemical manufacturing industry and also found in a broad range of food products, including infant formulas and baby foods. Previous reports have indicated that the adverse biological effects of furan, including its liver tumorigenicity, may be associated with epigenetic abnormalities. In the present study we investigated the persistence of epigenetic alterations in rat liver. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Platform:

GPL14797

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

97 Samples

Download data: TXT

(Submitter supplied) We investigated pulmonary mRNA profiles of female mice exposed to 0.018, 0.054 and 0.162 mg Printex 90 carbon black nanoparticles by single intra-tracheal instillation. We examined responses to these doses 1, 3 and 28 days after exposure, alongside respective controls. This study demonstrates widespread changes in inflammatory and acute phase response genes that persisted to 28 days at the highest exposure dose. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

67 Samples

Download data: TXT

(Submitter supplied) We investigated hepatic mRNA profiles of female mice exposed to 0.162 mg Printex 90 carbon black nanoparticles by single intra-tracheal instillation. We examined responses to this dose 1, 3 and 28 days after exposure, alongside respective controls. We show that genes part of the 3-hydroxy-3-methyl-glutaryl-CoA reductase pathway to be up-regulated by exposure to Printex 90.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL4134

30 Samples

Download data: TXT

(Submitter supplied) Molecular hydrogen is a hopeful agent for oxidative stress-related and/or inflammatory disorders. However, the molecular mechanism for these therapeutic effects of hydrogen still remains to be fully elucidated. We examined whether molecular hydrogen alters gene expression levels in normal mouse livers by DNA microarray analysis. We identified 140 mouse genes that were upregulated (31 genes) or downregulated (109 genes) after three weeks of the inhalation of 2% hydrogen-containing air with oral intake of hydrogen-rich water. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5642

1 Sample

Download data: GPR

(Submitter supplied) Transcriptomic data obtained by RNA-seq from male Fischer 344 rats treated with furan and 3-methylfuran were compared and contrasted. Microarray data from the same rats treated with furan was also used to compare microarray to RNA seq analysis.

Organism:

Rattus norvegicus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20084

172 Samples

Download data: TSV