GEO DataSets

(Submitter supplied) Honokiol (HNK), one of the main medicinal components in Magnolia officinalis, possesses antimicrobial activity against a variety of pathogenic bacteria and fungi.S. cerevisiae is a model eukaryote used for investigating the cellular and molecular mechanisms of anti-fungal drugs. To explore the molecular mechanism of its anti-fungal activity, we determined the effects of HNK on the mRNA expression profile of Saccharomyces cerevisiae using a DNA microarray approach.

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL2529

4 Samples

Download data: CEL

(Submitter supplied) In an approach to generate Saccharomyces cerevisiae strains with increased intracellular copper amounts for technical applications we over-expressed the copper transporter CTR1 and a variant of CTR1 with a truncation in the C-terminus after the 300 amino acids (CTR1 delta-300). We determined the copper sensitivity of the generated strains and used inductively coupled plasma spectrometry (ICP-OES and ICP-MS) analysis to investigate the effects of over-expression of both constructs under excess copper on the cellular content of different elements in S. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL16244

12 Samples

Download data: TXT

(Submitter supplied) Diclofenac is a widely used analgesic drug that can cause serious adverse drug reactions. We used Saccharomyces cerevisiae as model eukaryote to elucidate the molecular mechanisms of diclofenac toxicity and resistance. Although most yeast cells died during initial diclofenac treatment, some survived and started growing again. Microarray analysis of the adapted cells identified three major processes involved in diclofenac detoxification and tolerance. more...

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL2529

10 Samples

Download data: CEL

(Submitter supplied) The number and type of synthetic chemicals that are being produced worldwide continues to increase significantly. While these industrial chemicals provide numerous benefits, there is no doubt that some have potential to damage the environment and health. Toxicity must be evaluated and use must be carefully controlled and monitored in order to minimize potential damage. DNA microarray technology has become an important new technique in toxicology. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platforms:

GPL5996 GPL5994

3 Samples

Download data: GPR

(Submitter supplied) We employed CapitalBio Corporation to investigate the global transcriptional profiling of Saccharomyces cerevisiae treated with thymol. Keywords: gene expression array-based, count

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL6010

3 Samples

Download data

(Submitter supplied) ATP-binding cassette transporters Pdr5 and Yor1 from Saccharomyces cerevisiae control the asymmetric distribution of phospholipids across the plasma membrane as well as serving as ATP-dependent drug efflux pumps. Mutant strains lacking these transporter proteins were found to exhibit very different resistant phenotypes to two inhibitors of sphingolipid biosynthesis that act either late (aureobasidin A:AbA) or early (myriocin: Myr) in the pathway leading to production of these important plasma membrane lipids. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL6035

8 Samples

Download data: TXT

(Submitter supplied) We have used the budding yeast Saccharomyces cerevisiae to identify genes that may confer sensitivity in vivo to the antimalarial and cytotoxic agent cryptolepine. To this end, five S. cerevisiae strains, which differ in the condition of genes related to cell membrane integrity and to DNA damage repair, were exposed to several concentrations of cryptolepine. Results showed a relatively mild toxicity of cryptolepine for wild type strains, which increased by either increasing cell permeability (∆erg6 or ISE2 strains) or disrupting DNA damage repair (∆rad52 strains). more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL4069

8 Samples

Download data: TXT

(Submitter supplied) The strain bdf1Δ+HAL2 improved salt resistance of bdf1∆. To gain further insight into the mechanism of bdf1∆ salt sensitivity, DNA microarray analysis was performed to determine the reason for the salt sensitivity of bdf1∆ cells and the process of how HAL2 overexpression and HDA1 deletion improves salt resistance. Transcriptomic analysis under salt treatment (0.5 mol.L-1 NaCl for 45 min) was performed using four different strains: bdf1∆, W303, bdf1Δ+HAL2 and bdf1∆hda1∆. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL17201

5 Samples

Download data: TXT

(Submitter supplied) Reactive oxygen species, generated in vivo or exogenously encountered, constantly challenge living organisms. Oxidation of polyunsaturated fatty acids (PUFA), which are susceptible to oxidant attack, can lead to initiation of lipid peroxidation and in turn rapid production of toxic lipid hydroperoxides. Eukaryotic microorganisms such as Saccharomyces cerevisiae can survive harsh industrial conditions that contain high levels of the PUFA linoleic acid and its oxidised derivative, linoleic acid hydroperoxide (LoaOOH). more...

Organism:

Saccharomyces cerevisiae; Schizosaccharomyces pombe

Type:

Expression profiling by array

Platform:

GPL2529

6 Samples

Download data: CEL

(Submitter supplied) Genome-wide assays to test the interaction between two independently evolved mechanisms of fluconazole resistance Keywords: Comparative genomic hybridization

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL3458

26 Samples

Download data

(Submitter supplied) Abstract: Chemogenomic fitness assays were combined with a transcriptome analysis to understand both the mode of action and the mechanisms of resistance to Chitosan oligosaccharides (COS). COS are deacetylated chitin compounds, with antimicrobial properties, that are presumed to act by disrupting the cell membrane. The fitness assays identified 39 yeast deletion strains sensitive to COS and 21 suppressors of COS sensitivity. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL14716

36 Samples

Download data: XYS

(Submitter supplied) Invasive fungal infections (IFIs) are difficult to treat. Few effective antifungal drugs are available and many have problems with toxicity, efficacy and drug-resistance. To overcome these challenges, existing therapies may be enhanced using more than one agent acting in synergy. Previously, we have found amphotericin B (AMB) and the iron chelator, lactoferrin (LF), were synergistic against Cryptococcus neoformans and Saccharomyces cerevisiae. more...

Organism:

Saccharomyces cerevisiae S288C

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL21743 GPL20073

18 Samples

Download data: CSV

(Submitter supplied) We examined the gene expression changes resulting from the evolution of resistance in experimental populations of the yeast Saccharomyces cerevisiae subjected to two antifungal drugs, fluconazole (FLC) and amphotericin B (AmB). Fluconazole resistance may involve increased efflux or changes in sterol metabolism, while AmB resistance generally involves changes in sterol metabolism; for all of these types of resistance, the gene expression changes are extensive. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL3458

46 Samples

Download data: TXT

(Submitter supplied) Increasing evidence suggests that in disease-suppressive soils, microbial volatile compounds (mVCs) released from bacteria may inhibit the growth of plant-pathogenic fungi. However, the antifungal activities and molecular responses of fungi to different mVCs remain largely undescribed. In this study, we first evaluated the responses of pathogenic fungi to treatment with mVCs from Paenarthrobacter ureafaciens. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27812

6 Samples

Download data: XLSX

(Submitter supplied) Iron is an essential cofactor for enzymes involved in numerous cellular processes. We analyzed the metabolomes and transcriptomes of yeast grown in iron-rich and iron-poor media to determine which biosynthetic processes are altered when iron availability falls.

Organism:

Schizosaccharomyces pombe; Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL2529

8 Samples

Download data: CEL

(Submitter supplied) The main objective was to identify genes regulated after the BY4742 yeast cells were exposed to 0.125% for 5 or 10 min. The experiment was further valited by microbiological assays.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL9825

8 Samples

Download data: TXT

(Submitter supplied) In this study, we used the eukaryotic model Saccharomyces cerevisiae to better understand quinine’s mode of action and the mechanisms underlying the cell response to the drug. We performed a transcriptional profiling of the yeast response to a quinine concentration that exerted a very slight effect over cellular growth.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Dataset:

GDS3591

Platform:

GPL90

4 Samples

Download data: CEL, TXT

Analysis of Saccharomyces cerevisiae cells subjected to anti-malaria drug quinine (QN) at concentration that exerted a very slight effect on cellular growth. Results provide insight into the molecular mechanisms underlying QN action and the host response to the drug.

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL90

Series:

GSE18037

4 Samples

Download data: CEL

(Submitter supplied) Iron-resistant Saccharomyces cerevisiae mutant was obtained by evolutionary engineering selection strategy. The mutant obtained “M8FE” is much more resistant to iron stress than the reference strain which was used to select this mutant. Mutant can resist up to 35mM Iron* stress whereas the reference strain cannot. Whole-genome microarray analysis might be promising to identify the iron resistance mechanisms and stress response upon high levels of iron in the yeast cells. more...

Organism:

Saccharomyces cerevisiae

Type:

Expression profiling by array

Platform:

GPL16244

12 Samples

Download data: TXT