GEO DataSets

(Submitter supplied) The transcriptome, enhancer landscape and GR binding profile in primary mouse hepatocytes treated with glucagon and corticosterone

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

33 Samples

Download data: BEDGRAPH

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL19057 GPL13112

50 Samples

Download data: BEDGRAPH, CSV, FPKM_TRACKING

(Submitter supplied) During fasting the liver supplies the organism’s energy demands by producing glucose and ketones. Fuel production during fasting is temporally organized whereby glucose serves as the major fuel produced in short-term fasting while ketones are produced in longer fasts as gluconeogenic precursors deplete1. However, the regulatory process dictating this temporally-organized metabolic output is unexplored. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: FPKM_TRACKING

(Submitter supplied) During fasting the liver supplies the organism’s energy demands by producing glucose and ketones. Fuel production during fasting is temporally organized whereby glucose serves as the major fuel produced in short-term fasting while ketones are produced in longer fasts as gluconeogenic precursors deplete1. However, the regulatory process dictating this temporally-organized metabolic output is unexplored. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL13112

6 Samples

Download data: BEDGRAPH, CSV

(Submitter supplied) During fasting transcriptional programs lead to glucose and ketone production. The major TFs, their crosstalk and the enhancers driving it are unknown. We show that fasting massively reorganizes liver chromatin to expose 'fasting enhancers'. By tracking TF footprints, we implicated the major TFs regulating fuel production by two modules. The ketogenic module is executed by a temporally-organized TF cascade. more...

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL19057 GPL13112

38 Samples

Download data: BEDGRAPH, CSV

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

32 Samples

Download data: CEL

(Submitter supplied) Identified genes deregulated in mouse primary hepatocytes after modulation of expression/activity of FOXA2 and FXR in glucagon or insulin state

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

24 Samples

Download data: CEL

(Submitter supplied) Identified genes deregulated in mouse primary hepatocytes after glucagon and /or GW4064 treatment

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL16570

8 Samples

Download data: CEL

(Submitter supplied) During fasting, increases in circulating pancreatic glucagon maintain glucose balance by up-regulating hepatic gluconeogenesis. Triggering of the cAMP pathway stimulates the gluconeogenic program through the phosphorylation of CREB and via the de-phosphorylation of the CREB coactivator CRTC2. Hormonal and nutrient signals are also thought to modulate gluconeogenic genes by promoting epigenetic changes that facilitate assembly of the transcriptional machinery, although the nature of these modifications is unclear. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS5673

Platform:

GPL6246

10 Samples

Download data: CEL, CHP

Analysis of C57BL6/J primary hepatocytes depleted of either lysine acetyltransferase 2B (KAT2B) or WD repeat-containing protein 5 (WDR5) via shRNA knockdown, then stimulated with glucagon for 90 minutes. Results provide insight into the roles of KAT2B and WDR5 in hepatic gluconeogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 3 genotype/variation sets

Platform:

GPL6246

Series:

GSE47179

10 Samples

Download data: CEL, CHP

(Submitter supplied) Glucocorticoids play a key role in metabolic adaptation during stress, such as fasting and starvation. Excess and/or chronic glucocorticoid exposure, however, cause metabolic disorders that include insulin resistance dyslipidemia and hepatic steatosis. We previously showed that chronic glucocorticoid treatment elevates hepatic production of ceramides and sphingosine-1-phosphate (S1P). Ceramides are converted to sphingosine that is further converted to S1P. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL23479

6 Samples

Download data: TXT

(Submitter supplied) We mapped the genome-wide binding profiles of GR by using ChIP-Seq in livers from mice fed control or HFD diet after acute exogenous ligand (DEX) administration.

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

12 Samples

Download data: NARROWPEAK

(Submitter supplied) We mapped the genome-wide profiles of Histone H3K27 acetylation (two time points, ZT0 and ZT12) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL17021

8 Samples

Download data: BROADPEAK, GAPPEDPEAK

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL17021 GPL21103 GPL19057

176 Samples

Download data: BROADPEAK, GAPPEDPEAK, NARROWPEAK

(Submitter supplied) We mapped the genome-wide binding profiles of GR throughout the day/night cycle (ZT0-ZT4-ZT8-ZT12-ZT16-ZT20) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL19057 GPL21103 GPL17021

34 Samples

Download data: NARROWPEAK

(Submitter supplied) We mapped the genome-wide binding profiles of GR throughout the day/night cycle (ZT0-ZT4-ZT8-ZT12-ZT16-ZT20) by using ChIP-Seq in livers from control mice and mice fed High Fat Diet (HFD) for 12 weeks

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

122 Samples

Download data: TXT

(Submitter supplied) DNase-seq and ChIP-seq determine that C/EBP maintains chromatin accessibility in liver and facilitates glucocorticoid receptor recruitment to steroid response elements

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL9185

32 Samples

Download data: TXT, XLSX

(Submitter supplied) The binding of the glucocorticoid receptor (GR to GR regulatory elements (GREs) over constant treatment with 600nM corticosterone (Cort) changes over time in a locus-specific manner. We show this by comparing GR binding at 0h, 1h, and 12h after Cort treatment. This finding is further corroborated by the reduced dwell times of GR over time measured by single molecule tracking (SMT) as well as by the reduced RNA bust duration and frequency observed at the GR-mediated mouse mammary tumor promoter (MMTV) transcription sites (TSs).

Organism:

Mus musculus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL19057

4 Samples

Download data: BEDGRAPH, TXT

(Submitter supplied) Affymetrix whole genome microarray in female livers from virgins and 14.5dpc pregnant mice

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL20775

8 Samples

Download data: CEL, CHP