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Series GSE3748 Query DataSets for GSE3748
Status Public on Feb 08, 2007
Title Circadian liver_wt and Clock mutants
Organism Mus musculus
Experiment type Expression profiling by array
Summary One hundred ninety wildtype male C57BL/6J mice age 7-10 weeks were purchased from Jackson Laboratory and entrained to a 12:12 light:dark cycle for 2 weeks. Mice were placed in light-tight boxes on a 12:12 LD cycle for 4 weeks, then released into constant darkness. Starting 30 hours after entry into DD (CT18), tissues from 5 (skeletal muscle) or 10 (liver or SCN) wildtype mice were collected every 4 hours for 48 hours, for a total of 12 timepoints. At timepoints 34 through 58 hours in DD, tissues from age-matched male C57BL/6J Clock/Clock homozygous mutant mice that had been treated with the same light entrainment protocol as the wildtype were collected. Tissues were collected from 5 Clock/Clock mutants at each timepoint except for 34 and 46 hours after the onset of DD, when tissues from 10 Clock/Clock mice were collected and run as independent replicates. Mice were sacrificed by cervical dislocation, and the optic nerves were severed in complete darkness; brain dissection was performed using illumination from an infrared viewer (FJW Industries, Palatine, IL). SCNs were dissected out, pooled at a density of 5 per tube in 100 ?l RNAlater (Ambion, Austin, TX), frozen on dry ice, and stored at –80?C until use.
Keywords: time course
 
Overall design duplicates consisted of pools from independent mice
 
Contributor(s) McDearmon EL, Panda S, Andrews JL, Straume M, Walker JR, Esser KA, Hogenesch JB, Takahashi JS
Citation(s) 17360649, 17462724
Submission date Dec 06, 2005
Last update date Mar 16, 2012
Contact name John R Walker
E-mail(s) jwalker@gnf.org
Phone 858-812-1636
Organization name Genomics Institute of the Novartis Research Foundation
Lab Genetics Core
Street address 10675 John Jay Hopkins
City San Diego
State/province CA
ZIP/Postal code 92121
Country USA
 
Platforms (1)
GPL1073 GNF1M
Samples (38)
GSM86252 CT18;liver;NONE;C57/BL6;37445
GSM86253 CT18;liver;NONE;C57/BL6;37446
GSM86254 CT22;liver;NONE;C57/BL6;37447
This SubSeries is part of SuperSeries:
GSE3751 Circadian rhythm in clock mutants
Relations
BioProject PRJNA94307

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE3748_RAW.tar 218.1 Mb (http)(custom) TAR (of CEL)

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