GEO DataSets

(Submitter supplied) Validation of Lactobacillus plantarum WCFS1 transcriptome profile using RNA sequencing (direct cDNA sequencing and 3'-UTR sequencing) in comparison with DNA microarray as a reference platform.

Organism:

Lactiplantibacillus plantarum

Type:

Expression profiling by array

Platform:

GPL13984

4 Samples

Download data: TXT

(Submitter supplied) Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with 1.5 mM gallic acid (GA). Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals a massive induction of genes involved in transport and decarboxilation of gallic acid.

Organism:

Lactiplantibacillus plantarum WCFS1; Lactiplantibacillus plantarum

Type:

Expression profiling by array

Platform:

GPL15934

3 Samples

Download data: TXT

(Submitter supplied) Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with gallic acid (GA). Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals a massive induction of genes involved in transport and decarboxilation of gallic acid.

Organism:

Lactiplantibacillus plantarum; Lactiplantibacillus plantarum WCFS1

Type:

Expression profiling by array

Platform:

GPL15934

3 Samples

Download data: TXT

(Submitter supplied) A mutant of L. plantarumWCFS1 (deletion of lp_2991) was compared with the wildtype grown in standard MRS broth. Cells were sampled at OD1 for mRNA extraction.

Organism:

Lactiplantibacillus plantarum WCFS1

Type:

Expression profiling by array

Platform:

GPL9359

2 Samples

Download data: TXT

(Submitter supplied) Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to compare NGS-derived retinal transcriptome profiling (RNA-seq) to microarray and quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods and to evaluate protocols for optimal high-throughput data analysis. Methods: Retinal mRNA profiles of 21-day-old wild-type (WT) and neural retina leucine zipper knockout (Nrl−/−) mice were generated by deep sequencing, in triplicate, using Illumina GAIIx. more...

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11002

6 Samples

Download data: BAM, TXT, XLS

(Submitter supplied) Lactobacillus plantarum WCFS1 was differentially fermented in 29 different fermentations according to a factorial, combinatorial scheme that included variations in growth temperature (28 or 37C), NaCl concentration (0 or 0.3M), pH (5.2, 5.8, 6.4), as well as oxygen (N2 or air) and amino acid availability (1.1 or 2x concentration).

Organism:

Lactiplantibacillus plantarum

Type:

Expression profiling by array

Platform:

GPL13984

56 Samples

Download data: TXT

(Submitter supplied) Digestive Gland Samples: A manila clam oligo microarray platform (GPL10900) was used to profile gene expression in digestive gland of R. philippinarum. Total RNA was extracted from three (3) independent biological replicates of digestive gland for each sampling site, each consisting of tissue pools of five (5) animals. Statistical analysis with SAM (Significance Analysis of Microarray) identified1,127 probes differentially expressed. more...

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

12 Samples

Download data: TXT

(Submitter supplied) A manila clam oligo microarray platform (GPL10900) was used to profile gene expression in digestive gland of R. philippinarum sampled in four seasons in 4 different areas of Venice Lagoon. For each tissue, total RNA was extracted from four (4) independent biological replicates of digestive gland, each consisting of tissue pools of five (5) animals.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

64 Samples

Download data: TXT

(Submitter supplied) A manila clam oligo microarray platform (GPL10900) was used to profile gene expression in gills and digestive gland of R. philippinarum. For each tissue, total RNA was extracted from three (3) independent biological replicates of digestive gland and gills, each consisting of tissue pools of five (5) animals. Statistical analysis with SAM (Significance Analysis of Microarray) identified 8,257 probes differentially expressed between the two different tissues.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

6 Samples

Download data: TXT

(Submitter supplied) Four pools of digestive glands were treated as biological replicates in order to evaluate the repeatability of Ruditapes philippinarum oligo microarray platform.

Organism:

Ruditapes philippinarum

Type:

Expression profiling by array

Platform:

GPL10900

4 Samples

Download data: TXT

(Submitter supplied) Triplets of Lactobacullus plantarum strains were isolated from nine contrasting habitats. Without any passage through other culture media, isolation and cultivation were on model media that strictly reproduced the chemical and physical conditions and stressors of the habitats of origin. Here, we demonstrated how L. plantarum regulates and shapes its transcriptome in response to contrasting habitats. more...

Organism:

Lactiplantibacillus plantarum

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25156

36 Samples

Download data: TXT

(Submitter supplied) Sortases are transpeptidase enzymes that couple surface proteins to the peptidoglycan of Gram-positive bacteria. Several sortase-dependent proteins (SDPs) have been identified that are crucial for bacterial pathogenesis and, although less frequently, for the physiology of non-pathogenic bacteria. We found that an isogenic sortase A (srtA) deletion derivative (NZ7104) of Lactobacillus plantarum WCFS1 did not express any residual SrtA activity, i.e. more...

Organism:

Lactiplantibacillus plantarum

Type:

Expression profiling by array

Platform:

GPL13984

8 Samples

Download data: TXT

(Submitter supplied) Lactobacillus plantarum is a common inhabitant of mammalian gastrointestinal tracts and specific strains belonging to this species are marketed as probiotics intended to confer beneficial health effects. To assist in determining the physiological status and host-microbe interactions of L. plantarum in the digestive tract we assessed changes in the transcriptome of L. plantarum WCFS1 during colonization of the cecum of germ-free mice. more...

Organism:

Lactiplantibacillus plantarum WCFS1

Type:

Expression profiling by array

Platform:

GPL4318

18 Samples

Download data: IMAGENE

(Submitter supplied) Background: Whole transcriptome sequencing (RNA-seq) represents a powerful approach for whole transcriptome gene expression analysis. However, RNA-seq carries a few limitations, e.g., the requirement of a significant amount of input RNA and complications led by non-specific mapping of short reads. The Ion AmpliSeqTM Transcriptome Human Gene Expression Kit (AmpliSeq) was recently introduced by Life Technologies as a whole-transcriptome, targeted gene quantification kit to overcome these limitations of RNA-seq.To assess the performance of this new methodology, we performed a comprehensive comparison of AmpliSeq with RNA-seq using two well-established next-generation sequencing platforms (Illumina HiSeq and Ion Torrent Proton). more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17303

6 Samples

Download data: TXT

(Submitter supplied) Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with p-coumaric acid (p-CA). Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals a massive induction of genes involved in stress resistance and detoxification-related functions and a global shutdown of growth-associated processes.

Organism:

Lactiplantibacillus plantarum WCFS1; Lactiplantibacillus plantarum

Type:

Expression profiling by array

Platform:

GPL15934

3 Samples

Download data: TXT

(Submitter supplied) The aim of the study was to determine biological relevance of differentially expressed genes in Lactobacillus plantarum C2 during fermentation of plant substrates. Whole-transcriptome analysis based on customized microarray profiles has been used to determine altered transcription patterns in L. plantarum C2.

Organism:

Lactiplantibacillus plantarum

Type:

Expression profiling by array

Platform:

GPL20099

8 Samples

Download data: TXT

(Submitter supplied) We report a comparison of gene expression profiling microarray datasets generated from proliferating and serum-starved quiescent human normal diploid fibroblasts. 4 biological replicates of proliferative and quiescent fibroblasts were subjected to 4 individual dye-swap experiments. Data from these arrays were used in a publication to compare reproducibility between microarrays and RNA-seq; RNA-seq (2 replicates of each proliferative and quiescent fibroblasts) are available through the GEO (accession #: GSE65145). more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL19882

4 Samples

Download data: TXT

(Submitter supplied) We report the effects of Rapamycin treatment on the transcriptome of normal human dermal fibroblasts isolated from foreskin (designated 2DD). We sequenced mRNA from 2 replicates of proliferative (PRO) quiescent (QUI, serum starved) or treated with 500nM Rapamycin for 5 days (RAP). Comparative analyses with PRO transcripts a baseline indicate that genes that changed expression from Rapamycin treated fibroblasts are significantly different from those of quiescence cells. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL10999

6 Samples

Download data: TXT

(Submitter supplied) The level of the Central glycolytic gene regulator (CggR) was engineered in Lactobacillus plantarum NC8 and WCFS1 by over-expression and in-frame mutation of the cggR gene in order to evaluate its regulatory role on the glycolytic gap operon and the glyco

Organism:

Lactiplantibacillus plantarum; Lactiplantibacillus plantarum WCFS1

Type:

Expression profiling by array

Platform:

GPL4318

28 Samples

Download data: TXT

(Submitter supplied) Whole genome transcriptional profiling was used to characterize the response of Lactobacillus plantarum WCFS1 human isolate during challenge with resveratrol. Twelve independent experiments were performed and mixed at random in groups of four for total of three RNA samples. The transcriptional profile reveals a large reshape of nitrogen metabolism whic affects, among others, the pyridine and purine metabolisms as well as the metabolism of aminoacids related to thiol-specific oxidative stress response in this microorganism. more...

Organism:

Lactiplantibacillus plantarum

Type:

Expression profiling by array

Platform:

GPL22663

3 Samples

Download data: TXT