GEO DataSets

(Submitter supplied) Inhibition of hemicentin-1, which encodes a highly conserved ECM glycoprotein specifically expressed in muscle, leads to aberrant localization of internal parenchymal cells, including planarian stem cells (neoblasts) and differentiated cells, between the outer epidermis and sub-epidermal muscle.

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15553

6 Samples

Download data: TXT

(Submitter supplied) The transcriptome of a cell dictates its unique cell-type biology. We used single-cell RNA sequencing to determine the transcriptomes for essentially every cell type of a complete animal: the regenerative planarian Schmidtea mediterranea. Planarians contain a diverse array of cell types, possess lineage progenitors for differentiated cells (including pluripotent stem cells), and constitutively express positional information, making them ideal for this undertaking. more...

Organism:

Homo sapiens; Mus musculus; Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL19969 GPL21689

32 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of Schmidtea mediterranea planarians that have been amputated transversely and left to regenerate for different time points (UNIRR); the same experiment was performed on irradiated - neoblast depleted animals (IRR). The purpose of this experiment was to identify transcriptional changes that are induced within the first 12h following amputation. In order to distinguish between gene expression changes in the differentiated tissue and the neoblasts, a second set of time course data was created from irradiated animals

Organism:

Schmidtea mediterranea

Type:

Expression profiling by array

Platform:

GPL14150

10 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of Schmidtea mediterranea planarians that have been subjected to 2 weeks of runt-1 or control RNAi, amputated and RNA was directly collected at 5min, 9h, and 24h, or neoblasts (X1) were isolated from 9h wounded animals and subsequently RNA was extracted

Organism:

Schmidtea mediterranea

Type:

Expression profiling by array

Platform:

GPL14150

4 Samples

Download data: TXT

(Submitter supplied) How positional information instructs adult tissue maintenance is poorly understood. Planarians undergo whole-body regeneration and tissue turnover, providing a model for adult positional information studies. Genes encoding secreted and transmembrane components of multiple developmental pathways are predominantly expressed in planarian muscle cells. Several of these genes regulate regional identity, consistent with muscle harboring positional information. more...

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15553

177 Samples

Download data: TXT

(Submitter supplied) We used Nimblegen arrays with design 2007-11-06_Smed_ESTs_4_exp to measure changes in gene expression during a timecourse of regeneration of one side of the head in Schmidtea mediterranea flatworms. Following amputation of one side of the head, samples were collected from both the regeneration blastema and non-regenerating side of the head on days 2, 3, and 4 post-amputation. Gene expression in these samples was compared to that in non-regenerating control samples collected at the time of amputation.

Organism:

Schmidtea mediterranea

Type:

Expression profiling by array

Platform:

GPL10652

44 Samples

Download data: PAIR

(Submitter supplied) During adult homeostasis and regeneration, the freshwater planarian must accomplish a constant balance between cell proliferation and cell death, while also maintaining proper tissue and organ size and patterning. Yet how these ordered processes are precisely modulated, remains relatively unknown. Here we show that planarians use the downstream effector of the Hippo signalling cascade, yorkie (yki; YAP in vertebrates) to control a diverse set of pleiotropic processes in organ homeostasis, stem cell regulation, regeneration, and axial patterning. more...

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20150

6 Samples

Download data: XLS

(Submitter supplied) Freshwater planarians can be used as a model to investigate neuronal function and maintenance in adults in vivo. We knocked down expression for 2 transcription factors, then sequenced resulting animals to examine transcriptional changes that may have occurred.

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15553

9 Samples

Download data: XLS

(Submitter supplied) Difficulty of experimental accessing makes in vivo stem cell studies not easy in mammals and model systems with regenerative capability represent an alternative for the characterization of stem cell-specific genes. Planarians retain a population of pluripotent stem cells, the neoblasts, throughout their adult life. Neoblasts can be selectively destroyed by X-ray allowing us to compare stem cell-less organisms with wild-type worms. more...

Organism:

Dugesia japonica

Type:

Expression profiling by array

Platform:

GPL4000

28 Samples

Download data: GPR, TXT

(Submitter supplied) The goal of this study was to identify genes that were expressed in the protonephridial system. Because Six1/2(RNAi) and POU2/3(RNAi) animals lose the tubules, flame cells and accessory cells, these animals were expected to show decreased levels of the genes expressed in the nephridial system relative to control RNAi animals.

Organism:

Schmidtea mediterranea

Type:

Expression profiling by array

Platform:

GPL14150

12 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15553

212 Samples

Download data

(Submitter supplied) RNA sequencing of single cells from the midline posterior tip of the tail and posterior blastema in planarian Schmidtea mediterranea identified new posterior-pole enriched genes

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15553

152 Samples

Download data: TXT

(Submitter supplied) RNA sequencing identified various nr4A-regulated genes in the head and tail, including a large number of muscle-enriched genes and many positional control genes, most of which were down-regulated by nr4A inhibition.

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15553

48 Samples

Download data: TXT

(Submitter supplied) RNA sequencing identified new anterior-pole enriched genes encoding transcription factors, a secreted protein, and a transmembrane protein, including NR4A, a nuclear receptor gene that is important in patterning the planarian head and tail.

Organism:

Schmidtea mediterranea

Type:

Expression profiling by high throughput sequencing

Platform:

GPL15553

12 Samples

Download data: TXT