GEO DataSets

(Submitter supplied) To delineate the interaction of Candida glabrata with host immune cells, we performed genome-wide transcriptional profiling analysis on THP-1 macrophage-internalized wild-type and chromatin remodeling defective mutant (Cgrsc3-a∆ and Cgrtt109∆) yeasts. Genes implicated in ergosterol biosynthesis, and high-affinity iron uptake and homeostasis were found to be down-regulated in C. glabrata wild-type and mutant cells upon macrophage internalization. more...

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL15745

16 Samples

Download data: TXT

(Submitter supplied) To understand the host response of C. glabrata, we have employed the whole genome microarray expression profiling to identify the differentially regulated genes. For this, differentiated THP-1 cells were infected with C. glabrata wt, Cgvps34Δ and Cgyps(1-11)Δ cells for 6 h. Uninfected THP-1 cells, treated with PBS were taken as control. Total RNA was extracted using TriZol Reagent followed by ethonol precipitation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL21061

8 Samples

Download data: TXT

(Submitter supplied) To examine the response of Candida glabrata cells to iron-depleted and iron-repleted environmental conditions, transcriptional profiling analysis was carried out on wild-type and Cghog1∆ cells grown either in presence of BPS or ferric chloride. Genes involved in iron transport and homeostasis, oxidative phosphorylation, amino acid metabolic process and chromatin silencing were found to be differentially regulated.

Organism:

Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL19118

12 Samples

Download data: TXT

(Submitter supplied) To obtain a better understanding of molecular mechanisms underlying the DNA damage response in Δckb1 and Δckb2 mutants, transcriptome analyses were performed between WT, Δckb1, and Δckb2 strains. Comparative transcriptome analysis of Δckb1 and Δckb2 mutants showed similar transcriptional profiles in the presence and absence of MMS. The data also indicated that compared with WT, about 200 genes were up or downregulated in Δckb1 and Δckb2 strains with or without treatment with 0.01% MMS. more...

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33385

18 Samples

Download data: TXT

(Submitter supplied) The goals of this study are to compare C. glabrata transcriptome profiling (RNA-seq) upon exposure to hydrogen peroxide in order to assess the correspondent response. The role of the transcription factor Tog1 is clarified through comparison of the transcrptome profiles of WT and Tog1 mutant cells. mRNA profiles of WT and ∆tog1 were generated by deep sequencing, in duplicate, using Illumina HiSeq. The sequence reads that passed quality filters were analyzed with TopHat followed by HTSeq. more...

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26394

8 Samples

Download data: TXT

(Submitter supplied) In order to identify genes governed by Ada2, a transcription coactivator in C. glabrata, we compared the genome-wide expression profile in wild-type strain CBS138 and ada2 mutant via RNA sequencing. According to our analyses, 43 genes were down- and 443 genes were up-regulated in ada2 mutant when compared with the wild-type. The 43 down-regulated genes involved in ergosterol biosynthesis pathway (ERG6, ERG13), adhesin or adhesin-like genes (ICS2, AWP2, EPA7), heat shock genes (HSP30, SSB2) and other cellular functions. more...

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL19215

2 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL10713

33 Samples

Download data: TXT

(Submitter supplied) In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles.

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL10713

6 Samples

Download data: TXT

(Submitter supplied) In this study, we aimed to determine genome-wide changes in gene expression driven by seven individual CgPDR1 hyperactive alleles as compared to wild-type allele to identify i) the CgPdr1p target genes differentially expressed in presence of CgPDR1 hyperactive alleles and ii) potential virulence factor(s) regulated by CgPDR1 hyperactive alleles. Microarray experiments revealed a high number of genes (ranging from 80 to 400 genes) differentially regulated by individual CgPDR1 hyperactive alleles.

Organism:

Nakaseomyces glabratus CBS 138; Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL10713

27 Samples

Download data: TXT

(Submitter supplied) A set of 5,908 69-70 mers oligonucleotides that correspond to all C. glabrata putative open reading frames and some non-coding sequences was designed and synthesized at Pasteur Institute, France. Each oilgonucleotide was dissolved into 50% DMSO and spotted on UltraGAPsTM glass slides (Corning Incorporated, Acton, MA) in duplicate to generate whole genome microarray for C. glabrata. The microarray was printed at Biotechnology Research Institute/National Research Council of Canada in Montreal. more...

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL3922

12 Samples

Download data: GPR, TXT

(Submitter supplied) Our purpose is to clarify the global transcriptional profile in Candida glabrata phagocytized in human neutrophil.

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL8174

24 Samples

Download data: GPR

(Submitter supplied) Two C. glabrata related isolates recovered from the same patient undergoing azole therapy were characterized. The first isolate, BPY40, is azole-susceptibleand the second, BPY41, is azole-resistant. To determine whether the petite mutation conferred a selective advantage during host infection, the virulence of BPY40 and BPY41 was assessed in mice. Surprisingly, the petite mutant, even if showing in vitro growth deficiency as compared to BPY40, was more virulent than BPY40 both in intravenous and vaginal murine infection models. more...

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL10713

6 Samples

Download data: TXT

(Submitter supplied) Pleiotropic type I interferons (IFNs-I) fulfil multiple protective functions during infectious diseases, but can also exert detrimental effects for the host leading to immunopathology. Here, we report that IFNs-I promote the dysregulation of Fe homeostasis in macrophage populations and in the spleen during systemic infections with the intracellular pathogen Candida glabrata, leading to fungal survival and persistence. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL10787

32 Samples

Download data: TXT

(Submitter supplied) This study investigated the effect of milbemycons as efflux inhibitors and antifungal agents. Milbemycin oxims can inhibit growth of Candida glabrata and C. albicans. The effect of milbemycins on transcriptomes was inbvestigated.

Organism:

Nakaseomyces glabratus CBS 138; Candida albicans; Nakaseomyces glabratus

Type:

Expression profiling by array

Platforms:

GPL10713 GPL15960

14 Samples

Download data: TXT

(Submitter supplied) Transcriptome analysis of C. glabrata mutants evolved for improved tolerance to H2O2.

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL23894

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional time series of Candida glabrata under iron starvation (SD medium without Fe). Wild type and deletion mutants of the iron-related transcription factors Aft1 and Sef1, as well as of the iron uptake transporter Ftr1 as a positive control.

Organism:

Nakaseomyces glabratus; Nakaseomyces glabratus CBS 138

Type:

Expression profiling by array

Platform:

GPL10713

60 Samples

Download data: TXT

(Submitter supplied) The transcription profile of Candida glabrata grown under two different Niacin limitation conditions were determined. Condition 1 is comparing log phase C. glabrata cells (O.D. 0.5-0.6) grown in synthetic medium containing 0.016 uM versus 3.25 uM nicotinic acid (NA), a common form of Niacin. The NA concentration of 3.25 uM is the standard concentration in synthetic complete (SC) medium. Condition 2 is comparing log phase C. more...

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by array

Platform:

GPL3922

10 Samples

Download data: GPR

(Submitter supplied) To determine the effect of lack of two Histone H4 genes, CgHHF1 and CgHHF2, on global transcriptional profile, and on transcriptional response towards MMS exposure, RNA-Seq analysis was conducted on log-phase grown Candida glabrata wild-type and Cghhf1∆2∆ cells in the presence and absence of MMS. Comparative transcriptome analysis of wild-type and Cghhf1∆2∆ cells displayed deregulation of 303 genes due to the loss of CgHHF1 and CgHHF2 genes. more...

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL25492

8 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Nakaseomyces glabratus

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platforms:

GPL33482 GPL33483

24 Samples

Download data: TXT

(Submitter supplied) MNase-sequencing analysis led to identification of 56,637 to 71,823 nucleosomes under RPMI-growth or macrophage-internalized conditions. Mapping of dynamic nucleosomes revealed that 12 to 24% of total nucleosomes were altered in their position, occupancy or fuzziness. Notably, while the nucleosome position shift was the most common dynamic event, the nucleosome fuzziness was the least observed change. more...

Organism:

Nakaseomyces glabratus

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL33483

8 Samples

Download data: TXT