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Links from GEO DataSets

Items: 16

1.

RNA sequencing of hPSC-derived cardiac progenitors and endocardium

(Submitter supplied) We utilized a dual reporting hPSC line that identified cells expressing NKX2.5 and endothelal cells to characterize discrete milestones during cardiac and vascular differentiaiton. Comparing populations that express either or both reporters to human umbilical vein endothelial cells, we document a unique molecular phenotype in hPSC-derived endocardium that points toward an important role for Wnt signaling during vascular specification of cardiac progenitors.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
9 Samples
Download data: TXT
2.

Reprogramming of Endothelium Into Hematopoietic Progenitors by Defined Factors and Vascular Induction

(Submitter supplied) Generation of abundant engraftable hematopoietic cells from autologous tissues promises new therapies for hematologic diseases. Differentiation of pluripotent stem cells into hematopoietic cells results in emergence of cells that have poor engraftment potential. To circumvent this hurdle, we have devised a vascular niche model to phenocopy the developmental microenvironment of hemogenic cells thereby enabling direct transcriptional reprogramming of human endothelial cells (ECs) into hematopoietic cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL11154
16 Samples
Download data: TXT
3.

RNA-sequencing of human pluripotent stem-cell derived endothelial cells under control and Wnt-activating conditions

(Submitter supplied) Endothelial cells (ECs) in the central nervous system (CNS) acquire specialized barrier properties in response to extrinsic signals, with Wnt/β-catenin signaling coordinating multiple aspects of endothelial barrier function. We used human pluripotent stem cell (hPSC)-derived endothelial progenitor cells to profile the EC response to Wnt activation using multiple strategies, including the small molecule GSK-3 inhibitor CHIR 99021, and the CNS-derived ligands Wnt7a and Wnt7b.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL24676
20 Samples
Download data: XLSX
4.

Identification of Cardiovascular Lineage Descendants at Single Cell Resolution

(Submitter supplied) The transcriptional profiles of cardiac cells derived from murine embryos and from mouse embryonic stem cells (mESCs) have primarily been studied within a cell population. However, the characterization of gene expression in these cells at a single cell level may demonstrate unique variations that are not able to be appreciated as a pool. In this study, we aimed to establish a single cell quantitative PCR platform and perform side-by-side comparison between cardiac progenitors cells (CPCs) and cardiomyocytes (CMs) derived from mESC and mouse embryos. more...
Organism:
Mus musculus
Type:
Expression profiling by RT-PCR
Platform:
GPL19645
212 Samples
Download data: CSV
Series
Accession:
GSE64938
ID:
200064938
5.

Gene expression analysis of hESC-derived-MESP1 expressing progenitors

(Submitter supplied) A hESC MESP1-MCHERRY reporter line was used to isolate and study the molecular character of MESP1 expressing pre-cardiac progenitors, derived from hESC. MESP1 is a key-transcription factor for pre-cardiac mesoderm and is marking the progenitor for almost all cells of the heart. This reporter line was used to study cardiac differentiation and the derivation of early cardiac progenitors in vitro.
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL10558
9 Samples
Download data: TXT
Series
Accession:
GSE56721
ID:
200056721
6.

Gene Level Expression Profiling of Cardiac Progenitor Cells and Cardiomyocytes

(Submitter supplied) Transcriptome analysis of cardiac progenitor cells and cardiomyocytes The identification of cell surface proteins on stem cells or stem cell derivatives is a key strategy for the functional characterization, isolation, and understanding of stem cell population dynamics. Here, using an integrated mass spectrometry and microarray based approach, we analyzed the surface proteome and transcriptome of cardiac progenitor cells (CPCs) generated from the stage-specific differentiation of mouse and human pluripotent stem cells.
Organism:
Mus musculus
Type:
Expression profiling by array
Platform:
GPL6096
9 Samples
Download data: CEL, CHP
Series
Accession:
GSE103560
ID:
200103560
7.

Reconstitution of the human pancreatic niche stimulates differentiation of hESCs into beta cells and reveals new signals for pancreatic endocrine cell maturation

(Submitter supplied) RNA-seq was performed for hESC-derived endocrine progenitors (EPs) treated with WNT5A for short-term (12h) and long-term (5 days) to investigate the downstream targets of WNT5A in EPs. Sequencing of untreated cells and those treated with WNT5A for 12h, which are at EP stage, and for 5 days, which are at beta cell stage, allowed for observation of developmental changes during in vitro differentiation.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
8 Samples
Download data: XLS, XLSX
8.

Gene expression data from undifferentiated mouse embryonal carcinoma cell strains (MOE430A and MOE430B)

(Submitter supplied) Gene expression profiles of undifferentiated mouse embryonal carcinoma cell strains (P19, P19CL6, and 4 P19CL6 sublines) were obtained, using Affymetrix GeneChip Mouse Genome 430A and 430B. Heart diseases such as cardiac infarction damage cardiomyocytes and consequently lead to significant loss of the contractile capacity of the heart. To repair functions of the injured heart, a great deal of research has attempted to develop regenerative medicine using pluripotent stem cell-based cardiomyocytes as cell therapy products. more...
Organism:
Mus musculus
Type:
Expression profiling by array
Platforms:
GPL339 GPL340
60 Samples
Download data
Series
Accession:
GSE26875
ID:
200026875
9.

Functional Connection with Cardiac Tissues Confers Maturation Phenotypes in hPSC-Derived PHOX2B::GFP Expressing Sympathetic Neurons

(Submitter supplied) Neurons derived from human pluripotent stem cells (hPSCs) are a remarkable tool for modeling human neural development and diseases. However, it remains largely unknown whether the hPSC-derived neurons can be functionally coupled with their target tissues in vitro, which is essential for understanding inter-cellular physiology and further translational studies. Here, we demonstrate that hPSC-derived sympathetic neurons can be obtained from hPSCs and that the resulting neurons form physical and functional connections with cardiac muscle cells. more...
Organism:
Homo sapiens
Type:
Expression profiling by array
Platform:
GPL15207
11 Samples
Download data: CEL
Series
Accession:
GSE80689
ID:
200080689
10.

Single-cell transcriptomic profiles of hPSC-derived cardiac cells of various lineages and stages

(Submitter supplied) Using the hPSC system, we modelled multi-lineage (FHF, aSHF and pSHF) human embryonic cardiogenesis from mesoderm specification to cardiomyocyte differentiation and described these processes using single-cell RNA sequencing.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
10 Samples
Download data: MTX, TSV
Series
Accession:
GSE173486
ID:
200173486
11.

Generation of esophageal basal progeitor cells from human pluripotent stem cells

(Submitter supplied) We established an efficient protocol to generate esophageal epithelial progenitors (EPCs) from human pluripotent stem cells (hPSCs). Inhibition of TGFß and BMP signaling is required for the differentiation of hPSCs into EPCs which can be further purified with EPCAM and Integrin ß4. These purified EPCs express human fetal esophageal genes and recapitulate the normal development of the stratified squamous epithelium. more...
Organism:
Homo sapiens; Mus musculus
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL13112 GPL16791
11 Samples
Download data: TXT
Series
Accession:
GSE116272
ID:
200116272
12.

Retinoic acid signaling modulation guides in vitro specification of human heart field-specific progenitor pools

(Submitter supplied) He we report a systematic analysis of RA impact on human cardiovascular progenitor specification in orchestry with other signaling cues during early mesoderm formation. To approach this, we used a single cell transciptome profiling to dissect heterogeneity within human heart field-like cell populations and their derivatives. Our analysis enable us to identify known as well as a novel population of cells that can in vitro differentaite towards myocytic as well as epicardial lineages.
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing
Platform:
GPL18573
9 Samples
Download data: TAR
Series
Accession:
GSE197660
ID:
200197660
13.

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Homo sapiens
Type:
Expression profiling by RT-PCR; Other
Platforms:
GPL24607 GPL24606 GPL24605
36 Samples
Download data
Series
Accession:
GSE110563
ID:
200110563
14.

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [conventional RT-PCR]

(Submitter supplied) Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAprotect. Gene expression analysis was performed against clone- and passage-matched ground control samples.
Organism:
Homo sapiens
Type:
Expression profiling by RT-PCR; Other
Platform:
GPL24605
12 Samples
Download data: XLSX
Series
Accession:
GSE110562
ID:
200110562
15.

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [miRNA]

(Submitter supplied) Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAProtect, total RNA purification, and microRNA expression analysis against clone-, patient-, and passage-matched ground control samples.
Organism:
Homo sapiens
Type:
Other
Platform:
GPL24607
12 Samples
Download data: TXT
Series
Accession:
GSE110561
ID:
200110561
16.

Real-time quantitative PCR analysis of human cardiovascular progenitor cells flown aboard the International Space Station [Custom mRNA array]

(Submitter supplied) Adult and neonatal human cardiovacular progenitor cell clonal populations were flown aboard the ISS for 12 days prior to fixation in RNAprotect. Gene expression analysis was performed against clone-, patient-, and passage-matched ground control samples.
Organism:
Homo sapiens
Type:
Expression profiling by RT-PCR
Platform:
GPL24606
12 Samples
Download data: TXT
Series
Accession:
GSE110560
ID:
200110560
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