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Series GSE4976 Query DataSets for GSE4976
Status Public on Jun 06, 2006
Title Sex and line specific gene expression and alternative splicing in Drosophila revealed
Organism Drosophila melanogaster
Experiment type Expression profiling by array
Summary Many multi-exon genes are subject to alternative splicing, which is thought to increase phenotypic complexity by allowing a single locus to produce multiple functionally distinct proteins. However, genetic and developmental variation in alternative splicing has never been examined systematically. We therefore undertook a genome-wide analysis of sex- and genotypic-specific splicing in Drosophila in conjunction with sex- and line-specific transcription.
Keywords: microarray, sexual dimorphism, alternative splicing, genetical genomics, genetic variation
 
Overall design Experiments were conducted on flies from two standard strains of D. melanogaster: Oregon R [79] and 2b3 [80], and six randomly chosen recombinant inbred (RI) lines derived from these parental lines [81]. Each of the 8 lines was grown in four separate replicates of small mass-matings containing on average 20 adults, with a sex ratio of 1. We analyzed four independent biological replicates for each line and sex combination with a Cy3/Cy5 dye swap on the sexes.
Web link http://biocore.unl.edu/microarray/
 
Contributor(s) McIntyre LM, Bono LM, Genissel A, Westerman R, Junk D, Telonis-Scott M, Harshman L, Wayne ML, Kopp A, Nuzhdin SV
Citation(s) 16934145
Submission date Jun 05, 2006
Last update date Mar 16, 2012
Contact name Lauren M. McIntyre
E-mail(s) mcintyre@ufl.edu
Phone (352) 273-8024
Organization name University of Florida
Department Dept. of Molecular Genetics and Microbiology
Street address PO Box 100266; R2-293
City Gainesville
State/province FL
ZIP/Postal code 32610-0266
Country USA
 
Platforms (1)
GPL3809 Whole Genome, Alternative Splicing Drosophila Chip v3
Samples (50)
GSM111773 2B3 female D. melanogaster, biological rep1
GSM111774 2B3 female D. melanogaster, biological rep2
GSM111775 2B3 female D. melanogaster, biological rep3
Relations
BioProject PRJNA95481

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE4976_RAW.tar 96.1 Mb (http)(custom) TAR (of TXT)

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